LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis

Abstract

Background Long noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. However, the role and mechanism of lncRNA LINC01224 in CRC development are largely unknown. Methods LINC01224, Yin Yang 1 (YY1), microRNA (miR)-485-5p, and myosins of class VI (MYO6) levels were examined using quantitative reverse transcription polymerase chain reaction and western blotting. Functional analyses were processed through CCK-8, colony formation, flow cytometry, transwell, and xenograft analyses. Dual-luciferase reporter, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation, and pull-down assays were conducted to analyze the binding interaction. Results LINC01224 abundance was elevated in CRC tissue samples and cell lines. Elevated LINC01224 might indicate the lower 5-year overall survival in 52 CRC patients. LINC01224 was upregulated via the transcription factor YY1. LINC01224 knockdown restrained CRC cell proliferation, migration, and invasion and increased apoptosis. MiR-485-5p was sponged by LINC01224, and miR-485-5p downregulation relieved the influence of LINC01224 interference on CRC progression. MYO6 was targeted via miR-485-5p and regulated via LINC01224/miR-485-5p axis. MiR-485-5p overexpression suppressed CRC cell proliferation, migration, and invasion and facilitated apoptosis. MYO6 upregulation mitigated the role of miR-485-5p. LINC01224 knockdown decreased xenograft tumor growth. Conclusion YY1-induced LINC01224 regulates CRC development via modulating miR-485-5p/MYO6 axis.

miR-495-3p depresses cell proliferation and migration by downregulating HMGB1 in colorectal cancer

“…There are often complicated relationships when they work. For example, linc01224 targets the miR-485-5p/MYO6 axis to promote CRC progression [ 34 ], circ_0082182 functions as an oncogene in CRC by sponging miR-411 or miR-1205 to activate the Wnt/β-catenin pathway [ 35 ], and the circ0009910/miR-145-5p/PEAK1 axis contributes to the pathogenesis of CRC [ 36 ]. Our miR-495-3P may also be regulated by lncRNA or circRNA, which may become the content of our future research.…”

Predicting prognosis and immune responses in hepatocellular carcinoma based on N7-methylguanosine-related long noncoding RNAs

“…Some of the lncRNAs have already been confirmed to be significantly related to patient prognosis. Gu et al (2021 ) found that suppression of LINC01224 inhibited CRC cell proliferation, migration, and invasion while increasing apoptosis via the LINC01224/miR-485-5p axis. Qi et al (2019 ) discovered that an elevated level of lncRNA GABPB1-AS1 could inhibit GABPB1 transcription, resulting in the dysregulation of the gene encoding the peroxiredoxin-5 ( PRDX5 ) peroxidase and the suppression of cellular antioxidant capacity, implying that increased GABPB1-AS1 levels may be linked to improved prognosis in HCC patients.…”

The role of transcription factor Yin Yang‐1 in colorectal cancer

“…Without the suppression from miR‐361‐3p, overexpressed YY1 accelerates proliferation and glycolysis of CRC cells and the angiogenesis of human umbilical vein endothelial cells (HUVECs), meanwhile positively stimulating MIR31HG level in turn 38 . The similar positive feedback loops were detected as YY1/LINC00667/miR‐449b‐5p axis 39 and YY1/LINC01224/ miR‐485‐5p/myosins of class VI (MYO6) axis 62 . Cooperating with NF‐κB, YY1 directly binds to the promoter of LINC01578 to enhance its activity.…”