LINC01234 Accelerates the Progression of Breast Cancer via the miR-525-5p/Cold Shock Domain-Containing E1 Axis

Abstract

Backgrounds. Long noncoding RNAs (lncRNAs) are strongly associated with the development of breast cancer (BC). As yet, the function of LINC01234 in BC remains unknown. Methods. Using biological information, the potential lncRNA, miRNA, and target gene were predicted. LINC01234 and miR-525-5p expression in BC tissues was detected using quantitative real-time reverse transcription polymerase chain reaction. Fluorescence in situ hybridization was used to determine the distribution of LINC01234. Cell proliferation was analyzed using CCK-8 assay, colony formation, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and apoptosis evaluated using flow cytometry. Western blotting was used to evaluate protein expression. Dual-luciferase® reporter, RNA pull-down, and RNA immunoprecipitation assays were performed to analyze the binding relationships among LINC01234, miR-525-5p, and cold shock domain-containing E1 (CSDE1). Results. We screened out LINC01234, found to be significantly increased in BC tissues, associated with a poor prognosis, and positively correlated with tumor size of BC. Knockdown of LINC01234 suppressed BC cell growth and facilitated apoptosis. Dual-luciferase reporter®, RNA pull-down, and RNA immunoprecipitation assays confirmed that LINC01234 and CSDE1 directly interacted with miR-525-5p. Upregulation of miR-525-5p and suppression of CSDE1 inhibited BC cell growth and induced cell apoptosis. Conclusion. Upregulation of LINC01234 contributes to the development of BC through the miR-525-5p/CSDE1 axis. LINC01234 may be one of the potential diagnostic and treatment targets for BC.

Ensemble‐based classification using microRNA expression identifies a breast cancer patient subgroup with an ultralow long‐term risk of metastases

“…Six differentially expressed microRNAs (hsa‐miR‐212‐3p, MIMAT0000269; hsa‐miR‐499‐5p, MIMAT0002870; hsa‐miR‐525‐5p, MIMAT0002838; hsa‐miR‐519e‐5p, MIMAT0002828; hsa‐miR‐640, MIMAT0003310; and hsa‐miR‐942‐5p MIMAT0004985) were repetitively used by all methods during the classification of the OUH patients. For five of these six microRNAs a predictive or functional role in BC tumorigenesis had already been described, 35 , 36 , 37 , 38 , 39 , 40 , 41 but they had previously not been directly linked to risk of recurrence. However, only hsa‐miR‐212‐3p, hsa‐miR‐499‐5p, and hsa‐miR‐525‐5p were analyzed in the validation studies.…”

CSDE1: a versatile regulator of gene expression in cancer

“…In breast cancer cells, CSDE1 mRNA is targeted by miR-525-5p. LINC01234 acts as a sponge for miR-525-5p, leading to increased proliferation and reduced apoptosis ( 57 ) (Figure 5C ). CSDE1 mRNA was identified as the target of miR-132/212 in thyroid cancer cells ( 54 ) (Figure 5D ).…”