LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels

Abstract

Thyroid carcinoma (THCA) is a common type of endocrine system cancer and its current clinical treatment method is surgical resection. Long non-coding RNAs (lncRNAs) have been revealed to serve important roles in a variety of complex human diseases. Therefore, determining the association between lncRNAs and diseases may provide novel insight into disease-related lncRNAs, with the aim of improving disease treatments and diagnoses. Long intergenic non-protein coding RNA 1816 (LINC01816) was identified to be associated with the survival of patients with colorectal cancer using the IDHI-MIRW method. The present study aimed to investigate the role and molecular mechanism of LINC01816 in THCA. Analysis of datasets from The Cancer Genome Atlas database revealed that the upregulation of LINC01816 expression levels was associated with a variety of cancer types. Reverse transcription-quantitative PCR analysis demonstrated that compared with the normal thyroid tissues, the expression levels of LINC01816 were upregulated in THCA tissues. The results of wound healing and Transwell assays, and western blotting demonstrated that the overexpression of LINC01816 could strengthen the invasive and migratory abilities of THCA cells and enhance epithelial-mesenchymal transition progression. Analysis using the starBase website and dual-luciferase reporter assays identified that microRNA (miR)-34c-5p was a target of LINC01816. The overexpression of miR-34c-5p could inhibit the invasive and migratory abilities of THCA cells, in addition to inhibiting the cellular retinoic acid binding protein 2 (CRABP2) overexpression-induced effects on invasion, migration and EMT processes. In conclusion, the findings of the present study indicated that LINC01816 may be capable of sponging miR-34c-5p to upregulate CRABP2 expression levels, which subsequently promoted the invasion, migration and EMT of THCA cells. Therefore, targeting the LINC01816/miR-34c-5p/CRABP2 pathway may be an effective therapeutic approach for patients with THCA.

Novel lncRNA Panel as for Prognosis in Esophageal Squamous Cell Carcinoma Based on ceRNA Network Mechanism

“…LINC01087 is highly expressed in breast cancer [43]. LINC01816 was found to promote the migration of thyroid carcinoma cells [44]. Therefore, our findings still need to be verified through in the future in vivo and in vitro experiments as well as clinical practice.…”

Effects of particulate matter on endothelial, epithelial and immune system cells

“…En cultivo de células epiteliales basales alveolares humanas A549 se ha demostrado que la exposición al PM causa una sobreexpresión del gen NORAD, generando un deslizamiento mitótico que finalmente conlleva a una aneuploidía en la célula 58 . Por otro lado, en estas mismas células se ha demostrado la regulación positiva de LINC01816 59 , el cual es un ARN no codificante, cuya regulación positiva se ha asociado a varios tipos de cáncer 60 .…”

E-cadherin on epithelial–mesenchymal transition in thyroid cancer

“…On the contrary, CRABP2 (retinoic acid binding protein 2) is a necessary protein for tumor growth [ 150 ]. Overexpression of LINC01816 in thyroid carcinoma can target miR-34c-5p to act as a sponge, and then up-regulate the expression of CRABP2 and inhibit the expression of E-cadherin, thus promoting the occurrence of EMT [ 151 ]. Thirdly, LncRNAs can directly regulate the relevant signal pathways to regulate E-cadherin.…”