Lincomycin Protects Mice from Septic Shock in .BETA.-Glucan-Indomethacin Model

Nameda, Sachiko; Miura, Noriko N.; Adachi, Yoshiyuki; Ohno, Naohito

doi:10.1248/bpb.30.2312

Cited by 4 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lethal toxicity due to septic shock, elicited by a sequential administration of β-glucan and a nonsteroidal anti-inflammatory drug indomethacin, was described in mice 15,16 . In addition, these effects can be reversed by antibiotics 17,18 . Unfortunately, all these reports are from one laboratory and despite the 114 V. Vetvicka, J. Vetvickova Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we used four different types of glucan, differing in source, solubility and activity, Our finding showed that simultaneous treatment with glucan and indomethacin caused small decrease of phagocytic activity and IL-2 production. Two other tested parameters-blood glucose levels and colon length-both found to be significantly affected by this treatment 18 , were β-Glucan-indomethacin combination produces no lethal effects virtually unchanged. The reason why Epicor increased the colon length is unknown at this time.…”

Section: Figmentioning

confidence: 94%

“…The entire colon, from the coecum to the bottom of the rectum, was removed and colon length was measured as an indirect marker of inflammation 18 .…”

Section: Gastrointestinal Lengthmentioning

confidence: 99%

“…We have used the same conditions as described in 18 . In order to be sure we evaluate all possibilities, we included the experimental group where glucan was also administered orally.…”

Section: Figmentioning

confidence: 99%

“…A major problem was evidenced when glucan was applied simultaneously with indomethacin. Several papers reported the lethal side effects of indomethacin in glucan-treated mice [15][16][17][18] . These authors found that in glucan-treated mice, non-steroidal anti-inflammatory drug caused a gastrointestinal damage resulting in peritonitis by enteric bacteria and subsequent death.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Β-Glucan-Indomethacin COMBINATION PRODUCES NO LETHAL EFFECTS

Větvička¹,

Větvičková²

2009

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

View full text Add to dashboard Cite

Background:The most important quality of β-glucans and the reason why so much attention has been devoted to them are their physiological effects. They are typical biological response modifiers with pronounced immunomodulating activity. However, some questions about possible side effects remain.Aim: Several papers reported the lethal side effects of non-steroidal anti-inflammatory drugs in glucan-treated mice, probably due to the peritonitis by enteric bacteria. However, these results were never independently confirmed. The purpose of this study is to evaluate these claims using several different types of glucans.Methods: Effects of combined treatment with four different types of glucans and indomethacin were measured by evaluation of phagocytosis of HEMA particles by peripheral blood leukocytes and production of IL-6 in mouse serum. In addition, the level of blood glucose, colon length and survival after 30 days of treatment was measured.Results: Our finding showed that simultaneous treatment with glucan and indomethacin caused a small decrease of phagocytic activity and IL-2 production. Two other tested parameters-blood glucose levels and colon length-that had been found to be significantly affected by this treatment, were virtually unchanged. In the final, yet most important part of the study, we found absolutely no mortality, regardless of the type of glucan or the routes of glucan administration. Conclusion:No adverse negative effects due to simultaneous treatment with glucan and non-steroidal anti-inflammatory drug dose was found, despite testing two different routes of glucan administration and four different types of glucan.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Figmentioning

confidence: 94%

“…The entire colon, from the coecum to the bottom of the rectum, was removed and colon length was measured as an indirect marker of inflammation 18 .…”

Section: Gastrointestinal Lengthmentioning

confidence: 99%

“…We have used the same conditions as described in 18 . In order to be sure we evaluate all possibilities, we included the experimental group where glucan was also administered orally.…”

Section: Figmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Β-Glucan-Indomethacin COMBINATION PRODUCES NO LETHAL EFFECTS

Větvička¹,

Větvičková²

2009

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

View full text Add to dashboard Cite

show abstract

β-Glucan from Aureobasidum species inhibits fat accumulation in 3T3-L1 adipocyte differentiation

Kim

Kim²,

Cheong³

et al. 2015

Food Sci Biotechnol

View full text Add to dashboard Cite

The anti-fat accumulative effects of bacteriaderived β-glucan in 3T3-L1 adipocyte differentiation was investigated. 3T3-L1 preadipocytes were treated with β-glucan at 50 and 100 µg/mL during adipogenesis. Oil Red O staining was used to study expressions of the adipogenic genes PPARγ, ACC, and aP2. Adipogenesis of 3T3-L1 cells treated with β-glucan at 50 and 100 µg/mL was significantly (p<0.05) inhibited by 32.8 and 39.7%, respectively, compared with controls. Expressions of the PPARγ gene from 3T3-L1 cells treated with β-glucan at 50 and 100 µg/mL were significantly (p<0.05) decreased by 13.6 and 46.1%, respectively, ACC gene expression by 18.3 and 36.0%, respectively, and aP2 gene expression by 36.8 and 56.7%, respectively, compared to adipocyte controls. Bacteria-derived β-glucan inhibits fat accumulation at the cellular level that is mediated via decreases in expressions of the PPARγ, ACC, and aP2 genes.

show abstract