LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma

Abstract

AIM:To analyze the expression profiles of a human gastriccancer-related gene, GCRG123 , in human gastric signetring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123 . METHODS:In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues. Online software, including BLAST, Multalin and BLAT, were applied for bioinformatics analysis. National Center for Biotechnology Information (NCBI) and the University of California Santa Cruz (UCSC) databases were used for the analyses. RESULTS:The in situ hybridization signal appeared as blue precipitates restricted to the cytoplasm. Ten out of 15 cases of gastric signet ring cell carcinoma, normal gastric mucosal epithelium and pyloric glands showed high GCRG123 expression. Low GCRG123 expression was observed in gastric intestinal-type adenocarcinoma and normal gastric glands. Northern blotting revealed that GCRG123 was up-regulated in signet-ring cell carcinoma tissue but down-regulated in intestinal-type adenocarcinoma tissue. BLAST and Multalin analyses revealed that the GCRG123 sequence had 92% similarity with the ORF2 sequence of human long interspersed nuclear element retrotransposons (LINE-1, L1).BLAT analysis indicated that GCRG123 mapped to all chromosomes. GCRG123 was found to integrate in the intron-17 and -23 of Rb, 5' flanking region of IL-2 and clotting factor Ⅸ genes. LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma. World

Long interspersed nuclear elements (LINEs) show tissue-specific, mosaic genome and methylation-unrestricted, widespread expression of noncoding RNAs in somatic tissues of the rat

“…We also observed tissue-specific methylation patterns of P1-LINE with respect to the hypermethylated 5kb Msp I band in the lungs and thymus and the hypomethylated 2.8, 1.3, 0.89 and 0.4 kb well as in other diseases such as hemophilia, Duchenne Muscular Dystrophy (DMD), β-thalassemia, chronic granulomatous disease (CGD) and Coffin-Lowry Syndrome (CLS). [59][60][61][62][63][64][65][66][67][68][69][70][71] Therefore, expression of L1s is generally correlated with genome rearrangement, genomic instability, alterations in gene expression and diseases. 72 Recently, LINEs have been reviewed as a major source of genetic disposition to diseases in humans.…”