Line‐field confocal optical coherence tomography of xanthogranuloma: Correlation with vertical and horizontal histopathology

Lacarrubba, Francesco; Verzì, Anna Elisa; Puglisi, Davide Francesco; Broggi, Giuseppe; Caltabiano, Rosario; Micali, Giuseppe

doi:10.1111/cup.14067

Cited by 17 publications

(13 citation statements)

References 15 publications

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“…The usefulness of LC-OCT has been reported in a variety of skin disorders such as infectious diseases, autoimmune bullous diseases, skin tumours and other conditions. 1,3,[5][6][7][8][9][10][11][12][13][14] The aim of our study was to evaluate LC-OCT imaging in some common inflammatory skin disorders, that is plaque psoriasis, atopic eczema and lichen planus and to correlate the resulting features with histopathological findings.…”

Section: Introductionmentioning

confidence: 99%

Line‐field confocal optical coherence tomography of psoriasis, eczema and lichen planus: a case series with histopathological correlation

Verzì

Broggi

Micali

et al. 2022

Acad Dermatol Venereol

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Background Line-field confocal optical coherence tomography (LC-OCT) is a novel, non-invasive technique that provides in vivo, high-resolution images in both vertical and horizontal sections.Objectives The aim of the study was to evaluate LC-OCT imaging in some inflammatory disorders and to correlate the resulting features with histopathology.Methods The retrospective study included patients with histopathological confirmed diagnosis of plaque psoriasis, atopic eczema and lichen planus, who were imaged with LC-OCT before the biopsy. LC-OCT was performed with the commercially available LC-OCT device.Results A total of 15 adult patients with histopathologically proven plaque psoriasis (N: 5), atopic eczema (N: 5) and lichen planus (N: 5) were included. In all cases, LC-OCT allowed the in vivo recognition of the main microscopic features of the examined inflammatory skin disease, with a strong correlation with histopathology.Conclusions Although future studies on larger series of patients are necessary, LC-OCT, based on these preliminary findings, may represent a promising tool in inflammatory skin disorders with potential applications including enhanced diagnosis, biopsy guidance, follow-up and treatment monitoring.

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Section: Introductionmentioning

confidence: 99%

Line‐field confocal optical coherence tomography of psoriasis, eczema and lichen planus: a case series with histopathological correlation

Verzì

Broggi

Micali

et al. 2022

Acad Dermatol Venereol

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“…The interest of using LC-OCT in the diagnosis of skin disorders is growing. Reported applications include benign dermal melanocytic proliferations [ 4 ], skin tumors such as actinic keratoses, squamous and basal cell carcinoma (BCC) [ 1 , 5 , 6 , 7 , 8 ], sebaceous hyperplasia [ 9 ], xanthogranuloma [ 10 ], autoimmune bullous diseases [ 11 ], herpes infection [ 12 ], scabies [ 13 ], aquagenic keratoderma [ 14 ], Kaposi’s sarcoma [ 15 ], and circumscribed palmar hypokeratosis [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Line-Field Confocal Optical Coherence Tomography May Enhance Monitoring of Superficial Basal Cell Carcinoma Treated with Imiquimod 5% Cream: A Pilot Study

Verzì

Micali

Lacarrubba

2021

Cancers

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Line-field confocal optical coherence tomography (LC-OCT) is a novel, non-invasive technique for real-time skin imaging. Imiquimod (IQ) 5% cream is an immune response modifier currently approved for the treatment of small, superficial basal cell carcinoma (BCC). The aim of this study was to investigate if LC-OCT may be useful to enhance the treatment monitoring of BCC. Twenty superficial BCCs from 12 patients were treated with IQ 5% cream once daily, five days a week, for six weeks. Clinical and LC-OCT evaluations were performed at baseline and 4 weeks after the end of treatment. At the end of the study, 13 lesions showed a complete clinical and LC-OCT response, 4 lesions a partial clinical and LC-OCT response, and 3 lesions a complete clinical response but residual tumoral signs at LC-OCT. Our pilot study suggests that LC-OCT may represent a promising tool able to enhance the evaluation of the treatment response of BCCs to non-invasive treatments. In our case series, its use highlighted, through a detailed, fast, and complete examination of the treated area, three cases of residual BCC that otherwise would have gone undetected at clinical examination. Future studies on larger series of patients treated with different modalities and with a longer follow-up are advisable.

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“…Vertical sections of LC-OCT showed hyper-reflective structures corresponding to the ridge of melanocytic strands/cords/nests, separated by hyporeflective spaces of varying thickness ('wave-like' pattern, as previously described). 33 Large sparse dermal nests with large roundish monomorphic melanocytes were also evident (Fig. 2; Video S1).…”

Section: Benign Junctional Compound and Dermal Naevusmentioning

confidence: 95%

“…31 To overcome the depth limitations of RCM and the lack of cell resolution of OCT, LC-OCT was introduced. The main advantage of LC-OCT is that it permits 3D analysis of skin lesions, 2,[31][32][33][34][35] allowing an appreciation of both the architectural morphology and cytological aspects. Moreover, LC-OCT enables deeper imaging than RCM, with comparable cellular resolution.…”

Section: Introductionmentioning

confidence: 99%

Morphological evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

Pérez-Ánker

Puig

Alós

et al. 2022

Clinical and Experimental Dermatology

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Background Line‐field confocal optical coherence tomography (LC‐OCT) is a new in vivo emerging technique that provides cellular resolution, allows deep imaging (400 μm) and produces real‐time images in both the horizontal and vertical plane and in three dimensions. No previous description of different subtypes of melanocytic lesions and their correlation with histopathology and reflectance confocal microscopy has been reported. Aim To describe the features of melanocytic lesions by LC‐OCT and their correlation with histopathology and reflectance confocal microscopy (RCM) findings. Methods Selected melanocytic benign lesions and melanomas were imaged in vivo with RCM and LC‐OCT at the Fundación Hospital Clinic (Barcelona, Spain). A minimum area of 4 × 4 mm (block image) at four depths (stratum granulosum, suprabasal, layer dermoepidermal junction and upper dermis) were acquired with RCM and a minimum of three cubes with LC‐OCT. Horizontal, vertical sections and three‐dimensional (3D) cubes of LC‐OCT were matched with RCM (Vivablock two‐dimensional composite mosaic) and histopathology, with ~5 μm lateral resolution accuracy (the same cell nuclei were measured in X, Y and Z) and evaluated by three observers experienced in using RCM and histopathology. Results In total, 12 melanocytic tumours (2 in situ melanomas, 2 invasive melanomas, 4 atypical naevi, 2 intradermal naevi, 1 compound naevus and 1 junctional naevus) were included. High correlation with 5 μm accuracy between RCM and LC‐OCT was observed for each tumour. The 3D images of melanocytic lesions were obtained with cellular resolution and correlated with both RCM and histopathology, allowing an understanding of the architecture and precise correlation at the cellular level with RCM. Similarities between LC‐OCT and RCM for the described diagnostic features and architecture (nests of melanocytic cells, ringed and meshwork pattern, and cellular details of tumour cells as dendritic and pagetoid cells) were confirmed. The main advantage of diagnosis by RCM fixed probe was the ability to produce larger scans of the lesion using mosaicing compared with an LC‐OCT handheld probe. Conclusion LC‐OCT allows the architectural and cellular description of different types of melanocytic lesions. LC‐OCT showed high correlation with histopathology (vertical sections) and RCM (horizontal sections) in melanocytic lesions. Diagnostic criteria for RCM were similar to those for LC‐OCT.

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Line‐field confocal optical coherence tomography of xanthogranuloma: Correlation with vertical and horizontal histopathology

Cited by 17 publications

References 15 publications

Line‐field confocal optical coherence tomography of psoriasis, eczema and lichen planus: a case series with histopathological correlation

Line‐field confocal optical coherence tomography of psoriasis, eczema and lichen planus: a case series with histopathological correlation

Line-Field Confocal Optical Coherence Tomography May Enhance Monitoring of Superficial Basal Cell Carcinoma Treated with Imiquimod 5% Cream: A Pilot Study

Morphological evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

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