Line-scan Raman microscopy complements optical coherence tomography for tumor boundary detection

Abstract

Current technique for tumor resection requires biopsy of the tumor region and histological confirmation before the surgeon can be certain that the entire tumor has been resected. This confirmation process is time consuming both for the surgeon and the patient and also requires sacrifice of healthy tissue, motivating the development of novel technologies which can enable real-time detection of tumor-healthy tissue boundary for faster and more efficient surgeries. In this study, the potential of combining structural information from optical coherence tomography (OCT) and molecular information from line-scan Raman microscopy (LSRM) for such an application is presented. The results show a clear presence of boundary between myxoid liposarcoma and normal fat which is easily identifiable both from structural and molecular information. In cases where structural images are indistinguishable, for example, in normal fat and well differentiated liposarcoma (WDLS) or gastrointestinal sarcoma tumor (GIST) and myxoma, distinct molecular spectra have been obtained. The results suggest LSRM can effectively complement OCT to tumor boundary demarcation with high specificity.

Reagent- and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

“…Compared to traditional analytical methods, Raman and SERS methods offer several advantages. They require no reagents or separation, are non-invasive, are capable of qualitative and quantitative measurements, and provide molecular structure information [14][15][16][17][18][19][20][21]. In particular, SERS is a highly-sensitive Raman spectroscopic technique where Raman scattering is enhanced primarily by near-field electromagnetic enhancement due to localized surface plasmon resonance (LSPR) [22].…”

Noninvasive glucose sensing by transcutaneous Raman spectroscopy

“…[1][2][3] Based on inelastic scattering, Raman spectroscopy, as a type of vibrational spectroscopy, provides extremely rich molecular information about multiple chemical species present in a sample/specimen simultaneously. [4][5][6][7][8][9][10][11][12][13] The intensity of a Raman signal bears a linear relationship to the analyte concentrations, therefore, Raman spectroscopy can be used as a quantitative tool in concentration measurements as well. 1,4,5,[14][15][16][17] An ultimate goal in this field is to develop Raman spectroscopy-based techniques for biomedical applications through instrumentation, [18][19][20][21][22] plasmonic substrates, [23][24][25][26][27] devices, 28,29 assays, 30,31 and techniques.…”

Constrained regularization for noninvasive glucose sensing using Raman spectroscopy

“…2,7,14 Raman spectroscopy provides detailed quantitative information about sample composition, and is thus a promising technique for study of biological systems. [15][16][17][18][19][20][21] Glucose is a convenient analyte to study because its concentration can be conveniently altered and monitored in human volunteers. As shown below, numerical simulations and in vivo data collected noninvasively from the forearm of volunteers demonstrate that CR, when used with appropriate constraints, provides a calibration with signi¯cantly improved prediction accuracy compared to PLS.…”