Lineage determination of CD7+ CD5− CD2− and CD7+ CD5+ CD2− lymphoblasts: Studies on phenotype, genotype, and gene expression of myeloperoxidase, CD3ϵ, and CD3δ

Yoneda, Noriko; Tatsumi, E; Teshigawara, Keisuke; Nagata, Shinji; Nagano, T; Kishimoto, Yuji; Kimura, Takashi; Yasunaga, K; Yamaguchi, Nobuo

doi:10.1002/ajh.2830450408

Cited by 15 publications

(9 citation statements)

References 44 publications

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“…Cases 1-5 and 9-12 in this report are cases 1-5 and 8-11 in the previous report [21], respectively. This should be noted, since the results of the gene expression of myeloperoxidase, CD3e and CD36 are referred to for the consideration of the lineage of CD7+ CD5-CD2or CD7+ CD5 + CD2-blasts.…”

Section: Introductionsupporting

confidence: 66%

“…CD13 and/or CD33 antigen expression is very often present or induced in vitro in the blasts at the prothymic stage (Tables I, II), as has been shown [21]. The prolymphocytic leukemia cases in the peripheral stage T-lineage neoplasms (see Table IV) include one CD3+ CD8+ (cases 44), 2 CD3+ CD4+ (cases 41 and 48) and 2 CD3-CD4+ (cases 41 and 48) cases.…”

Section: Cell Surface Antigen Expression Including Cd45 Rnro Antigenmentioning

confidence: 57%

“…The in vitro cell surface induction of CD13 antigen in prothymic T-lineage neoplastic cells was investigated, as previously reported [21]. (%) of CD13 antigen by the blasts cultured for 2 days in, respectively, the absence and presence of TPA (2 ng/ml) is shown in parentheses.…”

Section: Antigen Expressionmentioning

confidence: 94%

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Expression pattern of CD45 RA/RO isoformic antigens in T‐lineage neoplasms

et al. 1995

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The expression of CD45 RA/RO antigen was investigated in neoplasms including cases expressing CD7 antigen as the sole pan-T antigen (n = 8), T-lineage acute lymphoblastic leukemia (ALL)/lymphoblastic lymphoma (LBL) at various stages of differentiation (n = 32), peripheral stage T-lineage leukemia (n = 10) and adult T-cell leukemia (ATL) (n = 14). The p56lck gene expression was also investigated in selected cases. The expression pattern of CD45 RA/RO antigen was defined as of RA, mixed, or RO type. All but one CD7+ CD5- CD2- case were of the RA type. The CD7+ CD5+ CD2- prothymic stage included seven RA and one mixed type cases. One CD7+ CD5- CD2+ case was of the RA type, but the other was of the RO type. The CD7+ CD5+ CD2+ prothymic stage included three RA and four mixed type cases. All seven CD3- CD4+ CD8+ (double-positive) thymic cases were of the RO type. The CD3+ CD4+ CD8+ (triple-positive) stage included two RO and three mixed-type cases. One CD3+ CD4+ CD8- late thymic case was of the mixed type. The peripheral stage cases included five RA, three RO, and two mixed type cases. All ATL cases were of the RO type. The expression of p56lck gene in the prothymic stage was less marked than that in the thymic stage. On the basis of these results, the following sequence of pattern of the CD45 RA/RO antigen expression along with T-lineage differentiation was reconstructed: prothymic stage [RA and mixed type]-->double-positive thymic stage [RO type]-->triple-positive thymic stage [RO and mixed type]-->peripheral stage [RA, mixed, and RO type]. While one RO-type CD7+ CD5- CD2- and one RO-type CD7+ CD5- CD2+ cases were not in accord with this sequence, the pattern of CD45 RA/RO antigen expression in most of T-lineage neoplasms could be determined by the respective stage of differentiation. The poor expression of the p56lck gene by the prothymic blasts compared with the thymic blasts may be related to the expression pattern of the CD45 RA/RO molecules, which exhibits phosphatase activity. The consistent RO-type expression in the ATL cases may reflect the activated status of the neoplastic T cells due to the presence of the HTLV-I gene. Alternatively, the target cells for HTLV-I-induced neoplastic transformation may possible be of the RO type.

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Section: Introductionsupporting

confidence: 66%

Section: Cell Surface Antigen Expression Including Cd45 Rnro Antigenmentioning

confidence: 57%

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Expression pattern of CD45 RA/RO isoformic antigens in T‐lineage neoplasms

et al. 1995

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“…The induction of CD13 and/or CD33 has been reported in the culture of T-lineage acute lymphoblastoid leukemia cells. 35 Conversely, the antigenic loss of CD16 might occur during the culture of NK cells in the presence of IL-2. However, it was not clear in the present study whether these phenotypic changes were caused by antigenic modulation during culture or by selective growth of the CD16 − , CD33 + , CD57 − population which has an advantage in cultures containing IL-2.…”

Section: Discussionmentioning

confidence: 99%

A novel natural killer cell line (KHYG-1) from a patient with aggressive natural killer cell leukemia carrying a p53 point mutation

et al. 2000

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“…CD13 positiveness in our cases related to the immaturity of Tcell. 16,17 CD 33 was positive in 2 cases. CD11b was positive in all patients.…”

Section: Discussionmentioning

confidence: 97%

T-lineage blast crisis of chronic myelogenous leukemia: simple record of 4 cases

Taroeno-Hariadi¹,

Tatsumi²,

Hardianti³

et al. 2005

Med J Indones

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Krisis blastik pada lekemia mielositik kronik (CML) bisa berasal dari lini granulosit, monosit, eritrosit, limfoid (sel B atau sel T), dan megakariositik. Krisis blastik seri limfoid biasanya berupa sel B dengan fenotipik sel Pre-B, di mana Ig permukaan belum diekspresikan. Krisis blastik dari sel T sangat jarang didapatkan. Tujuan penelitian : mendeskripsikan gambaran fenotipik, transkrip fusi bcr-abl, dan CD3 sitoplasmik, dan terminal deoxynucleotidyl transferase pada kasus-kasus CML dengan krisis blastik seri limfoid-T. Laporan kasus dari 4 kasus leukemia mielogenik kronik dengan krisis blastik sel-sel T yang dikumpulkan dalam kurun waktu 17 tahun (1987-2004). Kasus-kasus tersebut telah dilakukan pemeriksaan analisis fenotipik dan genotipik pada awal diagnosis ditegakkan. Kesemua kasus menunjukkan adanya t(9;22)(q34;q11). Sampel sel-sel mononuklear pasien yang disimpan dalam bentuk 10%DMSO diperiksa Reverse Transkripsi (RT) PCR BCR_ABL multiplex untuk mendeteksi transkrip fusi bcr-abl, PCR CD3ε untuk mendeteksi Cd3 sitoplasmik, dan PCR TdT untuk mendeteksi terminal deoxynucleotidyl transferase. Hasil analisis antigen permukaan sel pada awal diagnosis menunjukkan 1 kasus CD7+, CD5-, dan CD2-; 1 kasus CD7+, CD5+, dan CD2-; dan 2 kasus CD7+, CD5+, dan CD2+ yang menunjukkan bahwa semua sel T krisis blastik CML berada pada stadium pre dan protimik. Dua kasus menunjukkan hasil positip untuk transkrip bcr-abl b2a2, 1 kasus positip pada e1a2, dan 1 kasus negatip. RT PCR CD3ε menunjukkan hasil positip pada semua kasus dan RT PCR TdT hanya positip pada 1 kasus. Hasil yang dikumpulkan diharapkan dapat menjadi dasar analisis lebih lanjut pada kasus CML dengan krisis blastik sel-sel T.

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Lineage determination of CD7+ CD5− CD2− and CD7+ CD5+ CD2− lymphoblasts: Studies on phenotype, genotype, and gene expression of myeloperoxidase, CD3ϵ, and CD3δ

Cited by 15 publications

References 44 publications

Expression pattern of CD45 RA/RO isoformic antigens in T‐lineage neoplasms

Expression pattern of CD45 RA/RO isoformic antigens in T‐lineage neoplasms

A novel natural killer cell line (KHYG-1) from a patient with aggressive natural killer cell leukemia carrying a p53 point mutation

T-lineage blast crisis of chronic myelogenous leukemia: simple record of 4 cases

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