Lineage Differentiation Potential of Different Sources of Mesenchymal Stem Cells for Osteoarthritis Knee

Prajwal, Gollahalli Shivashankar; Jeyaraman, Naveen; Kanth, Krishna; Jeyaraman, Madhan; Muthu, Sathish; Rajendran, Sree Naga Sowndary; Rajendran, Ramya Lakshmi; Khanna, Manish; Oh, Eun Jung; Choi, Kang Young; Chung, Ho Yun; Ahn, Byeong‐Cheol; Gangadaran, Prakash

doi:10.3390/ph15040386

Cited by 7 publications

(4 citation statements)

References 145 publications

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“…have been employed for cartilage repair. The chondrogenesis and development of neo-cartilaginous tissues from such undifferentiated MSCs can be guided using growth factors, and other biophysical or biomechanical stimuli[ 96 , 97 ].…”

Section: Discussionmentioning

confidence: 99%

Does progress in microfracture techniques necessarily translate into clinical effectiveness?

Muthu,

Viswanathan,

Sakthivel

et al. 2024

World J Orthop

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BACKGROUND Multitudinous advancements have been made to the traditional microfracture (MFx) technique, which have involved delivery of various acellular 2nd generation MFx and cellular MFx-III components to the area of cartilage defect. The relative benefits and pitfalls of these diverse modifications of MFx technique are still not widely understood. AIM To comparatively analyze the functional, radiological, and histological outcomes, and complications of various generations of MFx available for the treatment of cartilage defects. METHODS A systematic review was performed using PubMed, EMBASE, Web of Science, Cochrane, and Scopus. Patients of any age and sex with cartilage defects undergoing any form of MFx were considered for analysis. We included only randomized controlled trials (RCTs) reporting functional, radiological, histological outcomes or complications of various generations of MFx for the management of cartilage defects. Network meta-analysis (NMA) was conducted in Stata and Cochrane’s Confidence in NMA approach was utilized for appraisal of evidence. RESULTS Forty-four RCTs were included in the analysis with patients of mean age of 39.40 (± 9.46) years. Upon comparing the results of the other generations with MFX-I as a constant comparator, we noted a trend towards better pain control and functional outcome (KOOS, IKDC, and Cincinnati scores) at the end of 1-, 2-, and 5-year time points with MFx-III, although the differences were not statistically significant (P > 0.05). We also noted statistically significant Magnetic resonance observation of cartilage repair tissue score in the higher generations of microfracture (weighted mean difference: 17.44, 95% confidence interval: 0.72, 34.16, P = 0.025; without significant heterogeneity) at 1 year. However, the difference was not maintained at 2 years. There was a trend towards better defect filling on MRI with the second and third generation MFx, although the difference was not statistically significant (P > 0.05). CONCLUSION The higher generations of traditional MFx technique utilizing acellular and cellular components to augment its potential in the management of cartilage defects has shown only marginal improvement in the clinical and radiological outcomes.

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Section: Discussionmentioning

confidence: 99%

Does progress in microfracture techniques necessarily translate into clinical effectiveness?

Muthu,

Viswanathan,

Sakthivel

et al. 2024

World J Orthop

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“…The cluster III mainly focused on therapeutic modalities, such as stem cells, repair, platelet-rich plasma, intra-articular injection, and transplantation. Many researchers had revealed the important pathological role of stem cell senescence in KOA and also provided a theoretical basis for endogenous stem cell therapy for KOA (Prajwal et al, 2022). In recent years, more and more researchers believed that exosomes secreted by MSCs also play a role in the treatment of osteoarthritis (Yu et al, 2022).…”

Section: Hot Topic In Basic and Clinical Researchmentioning

confidence: 99%

Research and publication trends on knee osteoarthritis and cellular senescence: a bibliometric analysis

Wang,

Yang,

Xiang

et al. 2023

Front. Physiol.

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Background: Cellular senescence is associated with age-related pathological changes, senescent cells promote the development of knee osteoarthritis. A better understanding between knee osteoarthritis and cellular senescence may enhance the effectiveness of therapies that aim to slow or stop the progression of this disease.Purpose: This study aimed to systematically analyze and visualize the publication trends, research frontiers and current research hotspots of knee osteoarthritis and cellular senescence by using bibliometrics.Methods: The publication search was performed on the Web of Science Core Collection database for documents published from 1992 to 2023. VOSviewer, Citespace, R package Bibliometrix and Microsoft Office Excel were used to study the characteristics of the publications. The publication number, countries, institutions, authors, journals, citations and co-citations, keywords were analyzed.Results: A total of 1,074 publications were analyzed, with an average annual growth rate of 29.89%. United States accounted for the biggest contributor, ranked first in publications and citations. Publications of this field were published in 420 journals, OSTEOARTHRITIS and CARTILAGE was the most influential. A total of 5,657 authors contributed to this research. The most productive author was Lotz, MK (n = 31, H-index = 22, Total citation = 2,619), followed by Loeser, R.F (n = 16, H-index = 14, Total citation = 2,825). However, the collaboration between authors was relatively weak. Out of the 1,556 institutions involved, 60% were from the United States. Scripps Research ranked first with 25 papers and a total of 2,538 citations. The hotspots of this field had focused on the pathomechanisms (e.g., expression, inflammation, apoptosis, autophagy, oxidative stress) and therapeutics (e.g., stem cell, platelet-rich plasma, transplantation, autologous chondrocytes, repair), and the exploration of Senolytics might be the important direction of future research.Conclusion: Research on the cross field of knee osteoarthritis and cellular senescence is flourishing. Age-related pathomechanism maps of various cells in the joint and the targeted medicines for the senescent cells may be the future trends. This bibliometric study provides a comprehensive analysis of this cross field and new insights into future research.

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“…MSCs derived from different sources share a common CD phenotype, morphology, and other features [ 8 , 9 ]. However, depending on the tissue of origin, MSCs can differ significantly in their biological properties, including their propensity for directed differentiation [ 10 , 11 ]. Subcutaneous adipose tissue, umbilical cord Wharton’s jelly, and deciduous tooth pulp MSCs are among the common candidates for the role of starting material in cell therapy/tissue engineering translational research due to their availability and lack of ethical constraints.…”

Section: Introductionmentioning

confidence: 99%

Cartilage-Specific Gene Expression and Extracellular Matrix Deposition in the Course of Mesenchymal Stromal Cell Chondrogenic Differentiation in 3D Spheroid Culture

Vakhrushev,

Basok,

Baskaev

et al. 2024

IJMS

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Articular cartilage damage still remains a major problem in orthopedical surgery. The development of tissue engineering techniques such as autologous chondrocyte implantation is a promising way to improve clinical outcomes. On the other hand, the clinical application of autologous chondrocytes has considerable limitations. Mesenchymal stromal cells (MSCs) from various tissues have been shown to possess chondrogenic differentiation potential, although to different degrees. In the present study, we assessed the alterations in chondrogenesis-related gene transcription rates and extracellular matrix deposition levels before and after the chondrogenic differentiation of MSCs in a 3D spheroid culture. MSCs were obtained from three different tissues: umbilical cord Wharton’s jelly (WJMSC—Wharton’s jelly mesenchymal stromal cells), adipose tissue (ATMSC—adipose tissue mesenchymal stromal cells), and the dental pulp of deciduous teeth (SHEDs—stem cells from human exfoliated deciduous teeth). Monolayer MSC cultures served as baseline controls. Newly formed 3D spheroids composed of MSCs previously grown in 2D cultures were precultured for 2 days in growth medium, and then, chondrogenic differentiation was induced by maintaining them in the TGF-β1-containing medium for 21 days. Among the MSC types studied, WJMSCs showed the most similarities with primary chondrocytes in terms of the upregulation of cartilage-specific gene expression. Interestingly, such upregulation occurred to some extent in all 3D spheroids, even prior to the addition of TGF-β1. These results confirm that the potential of Wharton’s jelly is on par with adipose tissue as a valuable cell source for cartilage engineering applications as well as for the treatment of osteoarthritis. The 3D spheroid environment on its own acts as a trigger for the chondrogenic differentiation of MSCs.

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Lineage Differentiation Potential of Different Sources of Mesenchymal Stem Cells for Osteoarthritis Knee

Cited by 7 publications

References 145 publications

Does progress in microfracture techniques necessarily translate into clinical effectiveness?

Does progress in microfracture techniques necessarily translate into clinical effectiveness?

Research and publication trends on knee osteoarthritis and cellular senescence: a bibliometric analysis

Cartilage-Specific Gene Expression and Extracellular Matrix Deposition in the Course of Mesenchymal Stromal Cell Chondrogenic Differentiation in 3D Spheroid Culture

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