Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles

Tolstik, Elen; Осминкина, Л. А.; Akimov, D. A.; Gongalsky, M. B.; Kudryavtsev, A. A.; Timoshenko, V. Yu.; Heintzmann, Rainer; Sivakov, Vladimir; Popp, Jürgen

doi:10.3390/ijms17091536

Cited by 35 publications

(26 citation statements)

References 79 publications

(127 reference statements)

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“…First, as we showed above, they can provide stronger TPEL and drastically stronger SHG signals, which is important for the implementation of nonlinear imaging modalities. Notice that TPEL has already been reported as an efficient information channel to track the presence of relatively small porous silicon‐based nanocrystals in vitro and in vivo . As follows from our data, larger crystals can provide much higher efficiency in such a mode of imaging (in our experiments, it was higher by 70%).…”

supporting

confidence: 74%

Bi‐Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics

Kharin

Lysenko

Rogov

et al. 2019

Advanced Optical Materials

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Having negligible toxicity compared to quantum dots based on compound semiconductors [1,2] and being capable of providing efficient imaging and therapeutic functionalities based on its unique physicochemical characteristics, [3][4][5] nanosilicon occupies a particularly important niche related to biological applications. The imaging functionality of nanosilicon typically employs photoluminescence (PL) of quantum-confined excitonic states in silicon (Si) nanocrystals (NCs) with sizes smaller than the exciton Bohr's radius (≈5 nm for the bulk crystalline Si (c-Si)), which enables tracking the presence of Si nanoparticles (NPs) in cells or tissues. [6][7][8][9][10][11][12][13] On the other hand, Si NPs can serve as efficient sensitizers of local heating under external stimuli to initiate hyperthermia-based therapies. As an example, Presenting a safe alternative to conventional compound quantum dots and other functional nanostructures, nanosilicon can offer a series of breakthrough hyperthermia-based therapies under near-infrared, radiofrequency, ultrasound, etc., excitation, but the size range to sensitize these therapies is typically too large (>10 nm) to enable efficient imaging functionality based on photoluminescence properties of quantum-confined excitonic states. Here, it is shown that large Si nanoparticles (NPs) are capable of providing two-photon excited luminescence (TPEL) and second harmonic generation (SHG) responses, much exceeding that of smaller Si NPs, which promises their use as probes for bi-modal nonlinear optical bioimaging. It is finally demonstrated that the combination of TPEL and SHG channels makes possible efficient tracing of both separated Si NPs and their aggregations in different cell compartments, while the resolution of such an approach is enough to obtain 3D images. The obtained bi-modal contrast provides lacking imaging functionality for large Si NPs and promises the development of novel cancer theranostic modalities on their basis.

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supporting

confidence: 74%

Bi‐Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics

Kharin

Lysenko

Rogov

et al. 2019

Advanced Optical Materials

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“…SIM is ideally suitable to perform live-cell studies due to the good temporal resolution and the minimum illumination power required. The exact intracellular localization of different nanomaterials has been determined thanks to the high particle localization accuracy of SIM [110][111][112][113] . In addition to the exact localization of the nanomaterial, SIM enabled the motoring of its degradation over time, as fragments of 100-200 nm could be imaged.…”

Section: [H2] Cellular Trafficking and Localizationmentioning

confidence: 99%

Super-resolution microscopy as a powerful tool to study complex synthetic materials

et al. 2019

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“…The potential of 3D‐resolved two‐photon‐actuated nanomedicine has been exploited via various nanomaterials such as semiconductor and carbon nanodots, polymeric, organic, silicon, gold, graphene and graphene oxide, silica hybrid, and other inorganic nanomaterials. What are the advantages of silica‐based nanoparticles?…”

Section: Two‐photon‐sensitive Organosilica Nanomaterialsmentioning

confidence: 99%

Two‐Photon‐Excited Silica and Organosilica Nanoparticles for Spatiotemporal Cancer Treatment

Croissant

Zink

Raehm

et al. 2018

Adv Healthcare Materials

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Coherent two-photon-excited (TPE) therapy in the near-infrared (NIR) provides safer cancer treatments than current therapies lacking spatial and temporal selectivities because it is characterized by a 3D spatial resolution of 1 µm and very low scattering. In this review, the principle of TPE and its significance in combination with organosilica nanoparticles (NPs) are introduced and then studies involving the design of pioneering TPE-NIR organosilica nanomaterials are discussed for bioimaging, drug delivery, and photodynamic therapy. Organosilica nanoparticles and their rich and well-established chemistry, tunable composition, porosity, size, and morphology provide ideal platforms for minimal side-effect therapies via TPE-NIR. Mesoporous silica and organosilica nanoparticles endowed with high surface areas can be functionalized to carry hydrophobic and biologically unstable two-photon absorbers for drug delivery and diagnosis. Currently, most light-actuated clinical therapeutic applications with NPs involve photodynamic therapy by singlet oxygen generation, but low photosensitizing efficiencies, tumor resistance, and lack of spatial resolution limit their applicability. On the contrary, higher photosensitizing yields, versatile therapies, and a unique spatial resolution are available with engineered two-photon-sensitive organosilica particles that selectively impact tumors while healthy tissues remain untouched. Patients suffering pathologies such as retinoblastoma, breast, and skin cancers will greatly benefit from TPE-NIR ultrasensitive diagnosis and therapy.

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Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles

Cited by 35 publications

References 79 publications

Bi‐Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics

Bi‐Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics

Super-resolution microscopy as a powerful tool to study complex synthetic materials

Two‐Photon‐Excited Silica and Organosilica Nanoparticles for Spatiotemporal Cancer Treatment

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