Linear dichroism and linearly polarised luminescence spectra of oriented samples collected on a new integrated instrument

Rodger, Alison

doi:10.1039/d4cc00038b

Chem. Commun.

2024

DOI: 10.1039/d4cc00038b

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Linear dichroism and linearly polarised luminescence spectra of oriented samples collected on a new integrated instrument

Alison Rodger

Abstract: Linearly polarised luminescence (LPL) has a wide range of potential applications giving optical and geometric parameters for oriented lumiphores. In this work we present the first wavelength scanned LPL spectra....

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Advantages of a synchrotron light source for fluorescence‐detected linear dichroism

Jones,

Rodger,

Hoffmann

2024

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Fluorescence‐detected linear dichroism (FD‐LD) enables one to collect linear dichroism spectra for oriented fluorophores in the presence of other absorbing species and light scattering. The experiment proceeds by scanning the excitation wavelength and using a filter to collect only emitted photons from the fluorophore. Thus, it has the potential to give data with enhanced selectivity and quality. By using a synchrotron radiation light source and fluorescence‐detection, we show data for a range of fluorophores in different orienting environments. Film and flow‐oriented FD‐LD spectra were collected down to 170 nm. Even for flow‐oriented liposomes, we have data collected down to 210 nm. For strongly scattering samples, for example, liposomes, FD‐LD has the clear advantage that scattering is absent for the longer wavelength fluorescence photons. The collimated and smaller beam size of the synchrotron radiation also gives rise to sharper and more well‐defined features in the spectra.

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Advantages of a synchrotron light source for fluorescence‐detected linear dichroism

Jones,

Rodger,

Hoffmann

2024

Chirality

Self Cite

View full text Add to dashboard Cite

show abstract

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Linear dichroism and linearly polarised luminescence spectra of oriented samples collected on a new integrated instrument

Abstract: Linearly polarised luminescence (LPL) has a wide range of potential applications giving optical and geometric parameters for oriented lumiphores. In this work we present the first wavelength scanned LPL spectra....

Cited by 1 publication

References 34 publications

Advantages of a synchrotron light source for fluorescence‐detected linear dichroism

Advantages of a synchrotron light source for fluorescence‐detected linear dichroism

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