Linear Element-Independent Meiotic Recombination in <i>Schizosaccharomyces pombe</i>

Wells, Jennifer L.; Pryce, David W.; Estreicher, Anna; Loidl, Josef; McFarlane, Ramsay J.

doi:10.1534/genetics.106.063818

Cited by 13 publications

(28 citation statements)

References 35 publications

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“…The rec10 gene codes for an LE component (Molnar et al 2003;Lorenz et al 2004). While deletion of rec10 strongly reduces DSB formation and recombination (Ellermeier and Smith 2005), some rec10 point mutants retain reduced recombination (Deveaux and Smith 1994;Pryce et al 2005;Wells et al 2006). The insertion mutation rec10-155TLEU2 used in this study truncates the gene for 103 codons at the 39-end and retains reduced meiotic recombination (Lin and Smith 1995;Wells et al 2006).…”

Section: Genotypementioning

confidence: 93%

Cohesin and Recombination Proteins Influence the G1-to-S Transition in Azygotic Meiosis inSchizosaccharomyces pombe

Doll

Molnár²,

Cuanoud³

et al. 2008

Genetics

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To determine whether recombination and/or sister-chromatid cohesion affect the timing of meiotic prophase events, the horsetail stage and S phase were analyzed in Schizosaccharomyces pombe strains carrying mutations in the cohesin genes rec8 or rec11, the linear element gene rec10, the pairing gene meu13, the double-strand-break formation genes rec6, rec7, rec12, rec14, rec15, and mde2, and the recombination gene dmc1. The double-mutant strains rec8 rec11 and rec8 rec12 were also assayed. Most of the single and both double mutants showed advancement of bulk DNA synthesis, start of nuclear movement (horsetail stage), and meiotic divisions by up to 2 hr. Only mde2 and dmc1 deletion strains showed wild-type timing. Contrasting behavior was observed for rec8 deletions (delayed by 1 hr) compared to a rec8 point mutation (advanced by 1 hr). An hypothesis for the role of cohesin and recombination proteins in the control of the G 1 -to-S transition is proposed. Finally, differences between azygotic meiosis and two other types of fission yeast meiosis (zygotic and pat1-114 meiosis) are discussed with respect to possible control steps in meiotic G 1 .

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Section: Genotypementioning

confidence: 93%

Cohesin and Recombination Proteins Influence the G1-to-S Transition in Azygotic Meiosis inSchizosaccharomyces pombe

Doll

Molnár²,

Cuanoud³

et al. 2008

Genetics

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“…The potential Rec114 ortholog in S. pombe, Rec7, also localizes to chromosomes, but Rec7 foci appear closely associated with chromosome axes and these foci do not form in mutants defective for the axis-associated protein Rec10 (Lorenz et al 2006). These differences may reflect the closer functional dependency between DSB formation and the cohesin-containing axes in S. pombe (Ellermeier and Smith 2005;Loidl 2006;Lorenz et al 2006;Wells et al 2006). …”

Section: Association Of Mei4 and Rec114 With Chromosomesmentioning

confidence: 99%

“…For example, genetic, cytological, and physical analyses suggest that Rec102 and Rec104 interact to form a single functional unit (Salem et al 1999;Jiao et al 2003;Kee et al 2004). Although this Rec102-Rec104 complex interacts with other DSB proteins, including Spo11, there are numerous differences between Rec102-Rec104 and the other proteins with respect to their genetic dependencies for protein-protein and protein-chromosome interactions Wells et al 2006) (discussed in more detail below). Moreover, whereas Mre11 and Spo11 appear to localize specifically to preferred sites of DSB formation as assessed by chromatin immunoprecipitation, Rec102 appears to be more broadly associated with both hotspot and non-hotspot regions (Borde et al 2004;Prieler et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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In most sexually reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein. In budding yeast, nine other proteins are also required for DSB formation, but roles of these proteins and interactions among them are poorly understood. We report here further studies of the behaviors of these proteins. Consistent with other studies, we find that Mei4 and Rec114 bind to chromosomes from leptonema through early pachynema. Both proteins showed only limited colocalization with the meiotic cohesin subunit Rec8, suggesting that Mei4 and Rec114 associated preferentially with chromatin loops. Rec114 localization was independent of other DSB factors, but Mei4 localization was strongly dependent on Rec114 and Mer2. Systematic deletion analysis identified protein regions important for a previously described two-hybrid interaction between Mei4 and Rec114. We also report functional characterization of a previously misannotated 5′ coding exon of REC102. Sequences encoded in this exon are essential for DSB formation and for Rec102 interaction with Rec104, Spo11, Rec114, and Mei4. Finally, we also examined genetic requirements for a set of previously described two-hybrid interactions that can be detected only when the reporter strain is induced to enter meiosis. This analysis reveals new functional dependencies for interactions among the DSB proteins. Taken together, these studies support the view that Mei4, Rec114, and Mer2 make up a functional subgroup that is distinct from other subgroups of the DSB proteins: Spo11-Ski8, Rec102-Rec104, and Mre11-Rad50-Xrs2. These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11.

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“…Complete deletion of the rec10 + coding region confers a phenotype indistinguishable from a rec12 mutation, thus Rec10 is essential for all DSB formation (Ellermeier and Smith 2005). A rec10 mutant (rec10-155) has been described which does not form linear elements but which does make DSBs, thus indicating that linear elements themselves are not essential for DSBs (Wells et al 2006). Rec7 foci are closely associated with Rec10-staining linear elements, and these foci do not form in rec10 mutants (Lorenz et al 2006).…”

Section: Dsb Proteins In S Pombementioning

confidence: 99%

“…Supporting this view, DSB formation is partially defective in S. cerevisiae mutants lacking certain chromosome structural components (Red1 and Hop1) (reviewed in Keeney 2001;Hunter 2007). As discussed in Section 3.2, an even more severe DSB defect is seen in S. pombe mutants lacking Rec10 (Lorenz et al 2004;Wells et al 2006). The molecular events that connect DSB formation with chromosome axes are not well understood.…”

Section: Higher Order Chromosome Structurementioning

confidence: 99%

Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis

Keeney

Genome Dynamics and Stability

311

359

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Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks made by the Spo11 protein, a relative of archaeal topoisomerase VI. This review summarizes recent studies that provide insight to the mechanism of DNA cleavage by Spo11, functional interactions of Spo11 with other proteins required for break formation, mechanisms that control the timing of recombination initiation, and evolutionary conservation and divergence of these processes.

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Linear Element-Independent Meiotic Recombination in Schizosaccharomyces pombe

Cited by 13 publications

References 35 publications

Cohesin and Recombination Proteins Influence the G1-to-S Transition in Azygotic Meiosis inSchizosaccharomyces pombe

Cohesin and Recombination Proteins Influence the G1-to-S Transition in Azygotic Meiosis inSchizosaccharomyces pombe

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis

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