Ecological environment has always been an important prerequisite while reflecting people and nature. The construction reflects the degree of development and civilization of a country as a whole, so it is related to the future of mankind. The deep integration of land use is a major breakthrough in solving the complex problems in the process of ecological civilization development and transformation. By establishing a "fusion" innovation and entrepreneurship ecological civilization system, this paper applies artificial intelligence and big data in the construction path of innovation and entrepreneurship ecological system from the perspective of land use and ecological suitability. Simulation studies were conducted in parasitic mode, biased symbiosis mode, asymmetric symbiosis mode, and symmetric symbiosis mode respectively through Matlab software. According to the results of the study, the subject size of the relevant subjects in the parasitic mode is only 70.43% of the subject size of the entrepreneurial enterprise. In the biased symbiosis model, the subject size of the relevant subject is 87.82% of the subject size of the entrepreneurial enterprise.