Linear or branched structure? Probing molecular architectures of fullerene?styrene copolymers by size exclusion chromatographs with online right-angle laser-light scattering and differential viscometric detectors

Huang, Yong’an

doi:10.1016/s0032-3861(04)00448-3

Cited by 2 publications

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“…OmniSEC software (Viscotek, USA) was used to calculate the number average molar mass (M n ) for dn / dc = 0.145 (β-glucans in NaN 3 solution). The procedure for calculating molar mass using OmniSEC software was reported previously [ 50 ].…”

Section: Methodsmentioning

confidence: 99%

Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation

et al. 2021

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We previously described the biosynthesis, isolation, and immunosuppressive activity of the selenium-containing polysaccharide fraction isolated from the mycelial culture of Lentinula edodes. Structural studies have shown that the fraction was a protein-containing mixture of high molar mass polysaccharides α- and β-glucans. However, which of the components of the complex fraction is responsible for the immunosuppressive activity non-typical for polysaccharides of fungal origin has not been explained. In the current study, we defined four-polysaccharide components of the Se-containing polysaccharide fraction determined their primary structure and examined the effect on T- and B-cell proliferation. The isolated Se-polysaccharides, α-1,4-glucan (Mw 2.25 × 106 g/mol), unbranched β-1,6-d-glucan, unbranched β-1,3-d-glucan and β-1,3-branched β-1,6-d-glucan (Mw 1.10 × 105 g/mol), are not typical as components of the cell wall of L. edodes. All are biologically active, but the inhibitory effect of the isolated polysaccharides on lymphocyte proliferation was weaker, though more selective than that of the crude fraction.

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Section: Methodsmentioning

confidence: 99%

Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation

et al. 2021

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“…OmniSEC software (Viscotek, USA) was used for calculation of number average molecular weight (M n ) for dn/dc = 0.150 (dextran in NaN 3 solution) (Antonini et al, 1964;Beri, Walker, Reese, & Rollings, 1993). The procedure for molar mass calculation by OmniSEC software has been reported previously by Huang et al (2004).…”

Section: High Performance Gel Permeation Chromatography Analysismentioning

confidence: 99%

Densitometric determination of carbohydrates: Application to purification and molecular weight determination of polysaccharide from Hericium erinaceum mushroom

Malinowska

Krzyczkowski

Łapienis

et al. 2010

Food Research International

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Linear or branched structure? Probing molecular architectures of fullerene?styrene copolymers by size exclusion chromatographs with online right-angle laser-light scattering and differential viscometric detectors

Cited by 2 publications

References 0 publications

Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation

Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation

Densitometric determination of carbohydrates: Application to purification and molecular weight determination of polysaccharide from Hericium erinaceum mushroom

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