Let f : X → B be a locally non-trivial relatively minimal fibration of genus g ≥ 2 with relative irregularity q f . It was conjectured by Barja and Stoppino that the slope λ f ≥ 4(g−1)g−q f . On the one hand, we show the lower bound λ f > 4(g−1)g−q f /2 , and also prove Barja-Stoppino's conjecture when q f is small with respect to g. On the other hand, we construct counterexamples violating the conjectured bound when g is odd and q f = (g + 1)/2.