Linearity of homologous series retention plots in reversed-phase liquid chromatography

Tchapla, Alain; Colin, Henri; Guiochon, Georges

doi:10.1021/ac00268a007

Cited by 187 publications

(113 citation statements)

References 22 publications

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“…These %RSD values (Table 2) were comparable to those observed in similar separations in the literature [49][50][51][52][53]. Despite the minimal variation observed in retention times and small reduction in R s values, the injection repeatability was excellent with respect to the variability, which is expected with monolithic columns [54].…”

Section: Investigating the Reduction Of Graphene Oxide-modified Polyhsupporting

confidence: 85%

“…Graphene, being more hydrophobic than GONPs, was expected to improve analyte retention in the stationary phase through increased hydrophobic interactions. As shown in Figure 6, however, the peak shape and elution order of the separation remained similar to the previous separation and similar to alkylbenzene separations in the literature [49][50][51][52][53]. An increase in retention time was observed as compared to the GONP polyHIPE, which can be linked to increased hydrophobic interactions between the analytes and the reduced stationary phase, however, this increase was small as a result of the low loading of GONP in the initial emulsion.…”

Section: Investigating the Reduction Of Graphene Oxide-modified Polyhsupporting

confidence: 83%

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Graphene Oxide Nanoparticles and Their Influence on Chromatographic Separation Using Polymeric High Internal Phase Emulsions

Choudhury

Duffy

Connolly³

et al. 2017

Separations

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This work presents the first instance of reversed-phase liquid chromatographic separation of small molecules using graphene oxide nanoparticle-modified polystyrene-divinylbenzene polymeric high internal phase emulsion (GONP PS-co-DVB polyHIPE) materials housed within a 200-µm internal diameter (i.d.) fused silica capillary. The graphene oxide nanoparticle (GONP)-modified materials were produced as a potential strategy to increase both the surface area limitations and the reproducibility issues observed in monolithic stationary phase materials. GONP PS-co-DVB polyHIPEs were found to have a surface area up to 40% lower than unmodified polymeric high internal phase emulsion (polyHIPE) stationary phases. However, despite having a surface area significantly lower than that of the unmodified material, the GONP-modified polyHIPEs demonstrated superior analyte adsorption properties. Reducing the GONP material did not have any significant impact on elution order or retention factor of the analytes, which was most likely due to low GONP loading attributed to the 250-nm GONPs utilised. The lower surface area of GONP-modified polyHIPEs provided similar separation efficiency and increased repeatability from injection to injection resulting in % relative standard deviations (%RSDs) of less than 0.6%, indicating the potential offered by graphene oxide (GO)-modified polyHIPES in flow through applications such as adsorption or separation processes.

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Section: Investigating the Reduction Of Graphene Oxide-modified Polyhsupporting

confidence: 85%

Section: Investigating the Reduction Of Graphene Oxide-modified Polyhsupporting

confidence: 83%

Graphene Oxide Nanoparticles and Their Influence on Chromatographic Separation Using Polymeric High Internal Phase Emulsions

Choudhury

Duffy

Connolly³

et al. 2017

Separations

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show abstract

“…This increment of k agrees with the results reported in previous papers. [40][41][42][43][44][45] This consistency probably proves the validity of the experimental retention data measured in this study. Figure 3a illustrates the linear correlations for the sample compounds between σax,mol 2 and tp at 298 K. As indicated in Eq.…”

Section: Determination Of Porositiessupporting

confidence: 78%

Moment Analysis of Chromatographic Behavior of Superficially Porous Particles

2011

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Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C18-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C18-particles were comparable to those of columns packed with a C18-silica monolith and full-porous C18-silica gel particles. The flow-rate dependence of HETP was hypothetically calculated by using moment equations to clarify the influence of the structural characteristics on the chromatographic behavior. The column efficiency of a column packed with the superficially porous particles is higher in the high flow-rate range than that with full-porous spherical particles. This is attributed to the smaller contribution of the intraparticulate mass transfer in the superficially porous particles to band broadening. The moment equations are effective for the quantitative analysis of chromatographic behavior of superficially porous particles.

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“…Curvature in the very low p range has been observed earlier and can be ascribed to the effect of the (butyl) end group on the total free energy of transfer of the first oligomers [32]. Deviations from linearity in the higher p range for other types of oligomers and homologues have been shown to occur at the critical carbon number and have been ascribed to a change in sorption mechanism from 'dissolution' in the bonded layer to physical adsorption [19,41]. Indeed, in some of the curves in Fig.…”

Section: Hplc Equipment 33 Determination Of Phase Ratio and Ksec Vamentioning

confidence: 72%

Study on the retention behaviour of low-molar-mass polystyrenes and polyesters in reversed-phase liquid chromatography by evaluation of thermodynamic parameters

Philipsen¹,

Claessens

Lind³

et al. 1997

Journal of Chromatography A

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. (1997). Study on the retention behaviour of low-molar-mass polystyrenes and polyesters in reversed-phase liquid chromatography by evaluation of thermodynamic parameters. Journal of Chromatography, A, 790(1-2), 101-116. DOI: 10.1016/S0021-9673(97)00739-5 General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. AbstractPolymers can be characterized under sorption conditions, to obtain information on molar mass and chemical composition. In order to get a better understanding of their retention behaviour under such conditions, the evaluation of thermodynamic parameters obtained from van't Hoff analyses on low-molar-mass polystyrenes (PS) and polyesters (PE) in various THF-water mixtures on a C column is described in this study. Linear van't Hoff behaviour was observed in almost all 18 cases. Negative values for both DH and DS were found for both PS and PE oligomers, which increase with increasing %THF. For DS this is explained from multi-site attachment effects. For PS, the non-linear relations between DH and DS, and degree of polymerization ( p) could be properly described by the Stockmayer-Fixman equation. Although less clear, similar trends were found for PE. For PS, evidence for penetration effects of oligomer chains into the bonded chains was obtained. Martin plots for both PS and PE were shown to be non-linear in all investigated eluent compositions. The extent of non-linearity is suggested to depend on the conformation of a polymer in solution. No distinct enthalpy-entropycompensation temperature (EECT) independent of p was found for PS, thus confirming the findings of an earlier study in which no exact molar mass independence was found under critical conditions. Further evaluation of EECT for PS oligomers revealed a retention mechanism independent of the binary eluent composition. This indicates that conclusions from this study can also be used for a qualitative understanding of sorption mechanisms in the gradient elution mode. Finally, for PS it was shown that DG equals zero under critical conditions, thus confirming theoretical predictions. © 1997 Elsevier Science B. V.

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Linearity of homologous series retention plots in reversed-phase liquid chromatography

Cited by 187 publications

References 22 publications

Graphene Oxide Nanoparticles and Their Influence on Chromatographic Separation Using Polymeric High Internal Phase Emulsions

Graphene Oxide Nanoparticles and Their Influence on Chromatographic Separation Using Polymeric High Internal Phase Emulsions

Moment Analysis of Chromatographic Behavior of Superficially Porous Particles

Study on the retention behaviour of low-molar-mass polystyrenes and polyesters in reversed-phase liquid chromatography by evaluation of thermodynamic parameters

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