Linezolid Inhibited Synthesis of ATP in Mitochondria: Based on GC-MS Metabolomics and HPLC Method

Ye, Xuemei; Huang, Aifang; Wang, Xianqin; Wen, Charles H.-P.; Hu, Lufeng; Lin, Gen‐Min

doi:10.1155/2018/3128270

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…Há relatos, em regimes de tratamento de mais de quatro semanas, de efeitos hematológicos mais extensos, neuropatia óptica e periférica e acidose lática, provavelmente por efeitos documentados de toxicidade mitocondrial dose dependente, mecanismo semelhante ao de inibição da síntese proteica bacteriana (Cheng;Lin;Wu, 2018;Ye et al,2018).…”

Section: Linezolida: Aspectos Farmacológicos Mecanismo De Ação Farmacocinética E Farmacodinâmica Efeitos Adversos E Cuidados Necessários unclassified

Revendo o tratamento para pneumonia causada por Staphylococcus aureus resistente à meticilina

Oliveira¹,

Lima²,

Sanches

et al. 2022

RSD

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) can cause several infections, both dermatological and invasive infections, such as pneumonia, empyema and sepsis. Due to its importance and pathogenicity, it is considered a worldwide public health problem, which implies the need to search for more efficient therapeutic regimens. Specifically for the treatment of MRSA pneumonia, vancomycin and linezolid are the main drugs used. Objective: The main objective of this study was to compare the two drugs in terms of their effectiveness in the treatment of MRSA pneumonia, through a review of the most recent aspects of the literature. Methodology: Through a narrative review, the pros and cons of each therapeutic intervention were investigated, as well as the individual pharmacological aspects and the pathophysiology of the bacterial infection in question. Conclusion: Finally, it was concluded that linezolid is superior to vancomycin, with better rates of reduced mortality and length of hospital stay, along with a lower risk of complications and adverse events, in addition to greater therapeutic flexibility, better cost-effectiveness and lower resistance rates.

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Section: Linezolida: Aspectos Farmacológicos Mecanismo De Ação Farmacocinética E Farmacodinâmica Efeitos Adversos E Cuidados Necessários unclassified

Revendo o tratamento para pneumonia causada por Staphylococcus aureus resistente à meticilina

Oliveira¹,

Lima²,

Sanches

et al. 2022

RSD

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“…Leakage of smaller intracellular molecules like amino acids or nucleotides can be measured by HPLC (Wenzel et al, 2015b;Ye et al, 2018). Smaller, ion-conducting pores can be monitored with different techniques.…”

Section: Membrane Poresmentioning

confidence: 99%

“…Inhibition of the respiratory chain typically leads to depletion of cellular ATP levels. This can be measured with commercially available chemiluminescence assays (Wenzel et al, 2014;Scheinpflug et al, 2017), or detected by HPLC or mass spectrometry (Dudley and Bond, 2014;Ye et al, 2018).…”

Section: Membrane-bound Processesmentioning

confidence: 99%

A How-To Guide for Mode of Action Analysis of Antimicrobial Peptides

Schäfer

Wenzel

2020

Front. Cell. Infect. Microbiol.

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Antimicrobial peptides (AMPs) are a promising alternative to classical antibiotics in the fight against multi-resistant bacteria. They are produced by organisms from all domains of life and constitute a nearly universal defense mechanism against infectious agents. No drug can be approved without information about its mechanism of action. In order to use them in a clinical setting, it is pivotal to understand how AMPs work. While many pore-forming AMPs are well-characterized in model membrane systems, non-pore-forming peptides are often poorly understood. Moreover, there is evidence that pore formation may not happen or not play a role in vivo. It is therefore imperative to study how AMPs interact with their targets in vivo and consequently kill microorganisms. This has been difficult in the past, since established methods did not provide much mechanistic detail. Especially, methods to study membrane-active compounds have been scarce. Recent advances, in particular in microscopy technology and cell biological labeling techniques, now allow studying mechanisms of AMPs in unprecedented detail. This review gives an overview of available in vivo methods to investigate the antibacterial mechanisms of AMPs. In addition to classical mode of action classification assays, we discuss global profiling techniques, such as genomic and proteomic approaches, as well as bacterial cytological profiling and other cell biological assays. We cover approaches to determine the effects of AMPs on cell morphology, outer membrane, cell wall, and inner membrane properties, cellular macromolecules, and protein targets. We particularly expand on methods to examine cytoplasmic membrane parameters, such as composition, thickness, organization, fluidity, potential, and the functionality of membrane-associated processes. This review aims to provide a guide for researchers, who seek a broad overview of the available methodology to study the mechanisms of AMPs in living bacteria.

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“…For example, phosphate anions known as mediators and/or metabolites in cellular signal transduction pathways (e.g., ATP hydrolysis) can be potential markers to indicate abnormal states leading to diseases. − Particularly, high concentrations of phosphates in human blood serum increase the risk of death caused by heart failure and myocardial infarction. − Given the association with the levels of phosphates and the significant symptoms, the easy qualification and quantification methods for oxyanions would encourage fundamental research in the fields of diagnosis and pharmacology. Conventionally, oxyanions have been assessed using large instrumental methods with high accuracy (e.g., ion chromatography, − liquid chromatography-tandem mass spectrometry, − and gas chromatography–mass spectrometry − ), while the miniaturization of those methods for on-site analysis is very hard to achieve. In this regard, molecular-sized chemosensors for oxyanions have been vigorously developed due to their naked-eye detectability of target molecules. − By considering the tetrahedral structure of hydrogen monophosphate (HPO 4 2– , Pi), elaborated receptor designs such as a pseudotetrahedral cleft, , a tripodal structure, , and macrocyclic- − or acyclic-dinuclear metal complexes have been reported.…”

Section: Introductionmentioning

confidence: 99%

Multi-Oxyanion Detection by an Organic Field-Effect Transistor with Pattern Recognition Techniques and Its Application to Quantitative Phosphate Sensing in Human Blood Serum

Mitobe

Sasaki

Tang

et al. 2022

ACS Appl. Mater. Interfaces

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We herein report an organic field-effect transistor (OFET) based chemical sensor for multi-oxyanion detection with pattern recognition techniques. The oxyanions ubiquitously play versatile roles in biological systems, and accessing the chemical information they provide would potentially facilitate fundamental research in diagnosis and pharmacology. In this regard, phosphates in human blood serum would be a promising indicator for early case detection of significant diseases. Thus, the development of an easy-to-use chemical sensor for qualitative and quantitative detection of oxyanions is required in real-world scenarios. To this end, an extended-gate-type OFET has been functionalized with a metal complex consisting of 2,2′-dipicolylamine and a copper(II) ion (CuII-dpa), allowing a compact chemical sensor for oxyanion detection. The OFET combined with a uniform CuII-dpa-based self-assembled monolayer (SAM) on the extended-gate gold electrode shows a cross-reactive response, which suggests a discriminatory power for pattern recognition. Indeed, the qualitative detection of 13 oxyanions (i.e., hydrogen monophosphate, pyrophosphate, adenosine monophosphate, adenosine diphosphate, adenosine triphosphate, terephthalate, phthalate, isophthalate, malonate, oxalate, lactate, benzoate, and acetate) has been demonstrated by only using a single OFET-based sensor with linear discriminant analysis, which has shown 100% correct classification. The OFET has been further applied to the quantification of hydrogen monophosphate in human blood serum using a support vector machine (SVM). The multiple predictions of hydrogen monophosphate at 49 and 89 μM have been successfully realized with low errors, which indicates that the OFET-based sensor with pattern recognition techniques would be a practical sensing platform for medical assays. We believe that a combination of the OFET functionalized with the SAM-based recognition scaffold and powerful pattern recognition methods can achieve multi-analyte detection from just a single sensor.

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Linezolid Inhibited Synthesis of ATP in Mitochondria: Based on GC-MS Metabolomics and HPLC Method

Cited by 12 publications

References 29 publications

Revendo o tratamento para pneumonia causada por Staphylococcus aureus resistente à meticilina

Revendo o tratamento para pneumonia causada por Staphylococcus aureus resistente à meticilina

A How-To Guide for Mode of Action Analysis of Antimicrobial Peptides

Multi-Oxyanion Detection by an Organic Field-Effect Transistor with Pattern Recognition Techniques and Its Application to Quantitative Phosphate Sensing in Human Blood Serum

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