Abstract-It is well known that the performance of Internet of Things application services is easy to be affected by internal noise of sensor, external environment interference and constriction of system resource. In order to solve the above problems, we studied the characteristics of bit error rate, outage ratio, signal to noise, and scale of relay sensors with channel state to choose the optimal transmission control scheme between direct mode and cooperative mode, building upon which we proposed the opportunistic cooperative automatic repeat request (ARQ) mechanism based on the rules and features of throughput ratio, average delay, packet error rate and energy efficiency, in consideration of retransmission time and channel quality. More importantly, the proposed opportunistic cooperative ARQ mechanism could set up the max retransmission time and control the progress of cooperative transmission according to the real-time network and sensor state, as well as Quality of Services (QoS) guarantee requirements of Internet of Things applications. The mathematical analyses and experiment results show that the proposed scheme is superior to the QoS provisioning schemes based on cooperative ARQ alone and direct transmission with channel state alone in terms of reliability, real time performance and life cycle of system, as well as energy efficiency.