Linkage analysis of Pseudomonas glycinea

Abstract

The IncP-1 plasmid R68 and variants R68.45 and R68.185 were tested for their chromosome donor ability in a selected recipient of Pseudomonas glycinea PGR12. It was found that these variants did not express their selected characteristic of increased donor ability over that of R68 or R68.5, our commonly used donor plasmids. Coinheritance analysis of a variety of crosses provides evidence of a linkage group comprising 11 loci.

Chromosomal genetics of Pseudomonas.

“…R68 evidently has all the other information necessary for such chromosome traisfer, because it displays Cma in P. aeruginosa PAT and P. glycinea (71,109,170,181). ECM plasmids have also been shown to be active in promoting chromosome transfer in other species of Pseudomonas, including P. putida (119; H. Dean, A. F. Morgan, and B. W. Holloway, unpublished data) and P. glycinea (50).…”

Conjugation-mediated genetic exchange in Legionella pneumophila

“…In this study we investigated the use of broad host range conjugative plasmids in developing a system for genetic exchange in L. pneumophila. Similar studies in other gramnegative bacteria have provided the basis for numerous genetic manipulations, including repair of chromosomal markers (28), linkage mapping (2,13,17,20,22), bacterial strain construction (15), transposon delivery (24, 26, 29), and the development of broad host range cloning systems (9,12).…”

Untitled

“…Following the first report on F -mediated chromosome mapping in Erwinia, reports on the conjugative transfer of IncP-1 group R plasmids to/from diverse bacteria, including plant pathogens and symbionts, and their use in chromosomal gene mapping came in rapid succession. By the decade's end, rudimentary chromosome linkage maps of a few phytopathogenic bacteria were published (14,31,63).…”