Linkage analysis of the fibroin gene in the silkworm, Bombyx mori.

Hyodo, Akio; Gamo, Takuma; Shimura, Kensuke

doi:10.1266/jjg.55.297

Cited by 21 publications

(11 citation statements)

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“…As shown in lanes 1 and 2, fibroin large subunits of the two strains exhibited each one band having different mobility. According to our classification of fibroin large subunit, the large subunits of J-131 and Nd (2) strains were classified as F and S pheno-type, respectively (Hyodo et al 1980b) . On the other hand, the large subunit of the hybrid (J-131/Nd(2)) silkworm exhibited two bands corresponding to F and S types.…”

Section: Resultsmentioning

confidence: 99%

“…A previous report from this laboratory demonstrated first that the structual gene of fibroin of the silkworm, Bombyx mori, was linked with the Nd (2) gene locating on the 23rd chromosome (Hyodo et al 1980b) . It has been reported that the Nd (2) mutation causes an apparent reduction of fibroin mRNA production when compared with a wild type (Suzuki and Suzuki 1974).…”

Section: Introductionmentioning

confidence: 99%

“…In this paper, we present that in the hybrid silkworm, there exist two fibroin alleles, SNd and Fnor according to our classification of fibroin phenotype, SNd allele being derived from Nd (2) strain and F"°r from J-131 strain (Hyodo et al 1980b), and that the amount of S fibroin mRNA and S fibroin accumulated in the posterior silk gland are about one fourth of those of F fibroin mRNA and F fibroin. This phenomenon might be caused by unequal transcription of two fibroin alleles.…”

Section: Introductionmentioning

confidence: 99%

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Gene expression of two fibroin alleles in the hybrid silkworm, J-131/Nd(2).

Hyodo

Ueda

Takei

et al. 1982

The Japanese journal of genetics

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Fibroin gene expression in the posterior silk gland of normal strain (J-131/ J-131), Nd strain (Nd(2)/Nd(2)) and their hybrid (J-131/Nd(2)) silkworms was compared on the levels of fibroin and fibroin mRNA production.The syntheses of both fibroin and fibroin mRNA in the Nd(2) and the hybrid silkworms were greatly reduced when compared with those of the normal silkworm. This result indicates that the Nd(2) gene represses dominantly fibroin gene expression, presumably at the level of transcription of fibroin gene. Estimation of gene products (fibroin and fibroin mRNA) from two fibroin alleles, Fno and SNd, of the hybrid silkworm, J-131/Nd(2), demonstrates that the expression of the fibroin allele SNd derived from Nd(2) mutant strain is more severely repressed than that of the fibroin allele Fnor derived from J-131 strain.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gene expression of two fibroin alleles in the hybrid silkworm, J-131/Nd(2).

Hyodo

Ueda

Takei

et al. 1982

The Japanese journal of genetics

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“…Fibroin is composed of one H-chain (MW 350,000) and one L-chain (MW 25,000), which are connected by disulfide bonds (Sasaki and Noda 1973;Gamo et al 1977;Shimura et al 1982). Genetic analysis by Hyodo et al (1980Hyodo et al ( , 1984 revealed that the H-chain gene (Fib-H) is linked with the Nd locus on the 25th chromosome3' and the L-chain gene (Fib-L) is linked with the U locus on the 14th chromosome. The Nd-s locus has been shown to be located on the 14th chromosome (Doira 1978).…”

Section: Introductionmentioning

confidence: 99%

“…3) Fib-H was originally assigned to the 23rd chromosome because of its linkage to Nd (Hyodo et al 1980) . The assignment was changed according to the recent notion that the correct locus for Nd is on the 25th chromosome (Doira 1983).…”

Section: Introductionmentioning

confidence: 99%