Linkage Data for Group Iv Markers in Neurospora

Maling, Barbara D.

doi:10.1093/genetics/44.6.1215

Cited by 11 publications

(4 citation statements)

References 4 publications

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“…The morphological mutants studied show the following distribution of 58 new loci in the various 1ink.age groups: Group I, 14 loci; 11, 6; 111, 6; IV, 9; V, 11; VI, 3; and VII, 9. The percent distribution is almost the same as the distribution of 122 biochemical loci (BARRATT and OGATA 1966), and approximately in proportion to the maximum established map lengths of the linkage groups from published maps (PERITINS 1959;PERKINS and ISHITANI 1959;MALING 1959;STRICKLAND, PERKINS and VEATCH 1959;PERKINS, GLASSEY and BLOOM 1962).…”

Section: Discussionmentioning

confidence: 52%

A SURVEY OF NEW MORPHOLOGICAL MUTANTS IN NEUROSPORA CRASSA

Garnjobst¹,

Tatum²

1967

Genetics

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HIS investigation of morphological mutants in Neurospora crassa was under-Ttaken to examine a wide spectrum of such strains and to obtain information regarding their number and distribution in each of the seven linkage groups. This information, as well as a comparative study of their morphological characteristics, is important both theoretically and practically in facilitating further investigations of the biochemical defects involved.The present report describes the linkage to known markers of 90 morphological strains of independent origin. Of these, 58 have designated as new loci; the remainder appear to represent alleles either of the new or previously described loci. The order of the genes has been determined only in group V (MORGAN, GARNJOBST and TATUM 1967).The differences between strains in type of mycelial growth, both from the ascospore and upon transfer, and the distinct differences between some strains in hyphal morphology have suggested a new and practical classification of the morphological mutants. Six classes are defined, illustrated, and discussed. MATERIALS A N D METHODS Strains:The conidia used in the ultraviolet irradiation experiments were from the inositol requiring strains 89601-3-34, 89601-4-10~, or 89601-4-12A, all of the heterocaryon genotype C , D; E (WILSON and GARNJOBST 1966). These strains were derived from inos (89601a, from DR. NORMAN GILES) through crosses with wild type SR17-3A, as indicated below. SR17-3A was obtained from a cross of SY7A with LINDEGREN 25a.

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Section: Discussionmentioning

confidence: 52%

A SURVEY OF NEW MORPHOLOGICAL MUTANTS IN NEUROSPORA CRASSA

Garnjobst¹,

Tatum²

1967

Genetics

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“…gul-3 (represented by one allele) lies on the right arm of linkage group IV. On the basis of the data presented in Table 2 and the general map of this region (MALING 1959;PERKINS, GLASSEY and BLOOM 1962), it can be located half way between pyr-2 and cot.…”

Section: Resultsmentioning

confidence: 95%

MODIFIERS OF THE cot GENE IN NEUROSPORA: THE GULLIVER MUTANTS

Terenzi¹,

Reissig²

1967

Genetics

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ORPHOLOGICAL mutants were originally studied in Neurospora on account of their value as genetic markers. Recently, their potential usefulness for the analysis of differentiation has received some attention (for example MUR-RAY and SRB 1962; SUSSMAN, LOWRY, and DURKEE 1964; BACHMANN 1964;BRODY and TATUM 1966). ThiAs is a promising development because a well characterized group of morphological mutants may become instrumental in the elucidation of the circuits involved in the programming of morphogenesis, just as the study of regulatory mutants led to the elucidation of the circuits involved in the control of metabolism (JACOB and MONOD 1961 ) .This paper deals with a group of morphological mutants which might be useful for the approach outlined above. These mutants act as modifiers of the temperature sensitive colonial gene cot, giving rise to giant ("gulliver") colonies. MATERIALS A N D METHODS Strains:Standard markers were obtained from the Fungal Genetics Stock Center, and combined with the cot gene by crossing. cot (isolation No. C102t) determines colonial growth at temperatures above 32"C, but unrestricted, wild-type growth at lower temperatures (MITCHELL and MITCHELL 1954). Its wild-type allele, on the other hand, allows unrestricted growth regardless of the temperature. Gulliver (gul) mutants were usually isolated by ultraviolet (UV) irradiation of microconidia1 ( p e p ) cot strains. The UV doses applied allowed survival of 0.2 to 13% of the population. Media ~l z d incubation temperatures:Fries minimal medium (BEADLE and TATUM 1945) was used for plating at 32 to 34°C. Occasionally, a mixture of 0.1% sorbose, 0.05% glucose and 0.05% fructose was substituted for sucrose in order to obtain colonial growth of cot+ strains. The medium of WESTERGAARD and MITCHELL (1947), with 1% sucrose, was used for crosses at 20 to 25°C. The medium of VOCEL (1956) was used for stock cultures at 20°C. Media were supplemented as required.Heterocaryons: The input strains, carrying different auxotrophic markers, were inoculated jointly on minimal medium plates. Single inoculations served as controls.Crosses: In all guZ+ x gul crosses, gul+ was the protoperithecial parent. Ascopores were collected and treated for 5 min with a solution of sodium hypochlorite. The ascospores were washed in water by centrifugation and resuspended in semisolid agar (0.2%) for counting, activating by heat-shock, diluting and plating. Germination was scored using a dissection micro-' .\'did 11)grank from the Consejo Nilclonal de Investigaciones Cientificas y Tkcnicas (Repdblica Argentina

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“…Right of ilv-3 (9%) (579). Left ofpan-i (1 to 2%), chol-i (1%), and cot-i (2 to 6%) (633). (692) Uses adenine or hypoxanthine (682).…”

Section: Microbiol Revmentioning

confidence: 99%

“…(1172) chol-1: choline-1 IVR. Linked to ad-6 (1%), probably to the right (633). (482) Requires choline (470).…”

Section: Aza: Azapurine Resistantmentioning

confidence: 99%

Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

263

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I I ' ~~~~T h Ñ CiOr(r r N z ADDITIONAL LOCI IN LINKAGE GROUP ILinked to mt (8 to 12%) Linked to mt, acr-3 (5%) Right of arg-i (5/29) Linked to so and aro-8 Right of un-18 Between ad-5 and the centromere Between arg-i (4%) and his-3 (I to 2%) Between In(H4250) and T(39311) Left of mt (23%) Linked to mt (2%) and aza-i (39%o) Left of nit-2 (30%) Left of mt (14%) Linked to un-i (9%), probably to the right Linked to hom (1%), probably to the right Linked to mt (17 to 22%) Between T(NM103) and al-2 (18%) Between T(4540) and cr-2Between thi-i (12 to 20%/6) and ad-9 (5%) Linked to ad-5 (1/54), probably to the left Linked to mt (6%) Linked to cys-5 (<1%), probably to the right Right of ad-9 (12%), linked to al (0/76) Right of his-3 (2%) Between the T(AR173) breakpoints Linked to al-2 (10%) and nic-i (1 to 5%) Linked to ad-9 (0/44); right of thi-i (2%) Linked to mt (5%), probably to the left Linked to al-I (10%) Right of os-i (3%) Between nit,i (5 to 19%) and al-I (6 to 19%io) Linked near arg-i Left of al-2 (25%o) Left of mt (16%) Right of nic-2 (6%) Linked to ad-9 (2%), probably to the right Left of cr-i (15%) Left of mt (10%) Probably between his-2 (3%) and cr-i (3%) Between T(NM103) and the right tip Between mt (20%) and arg-i (1%) Between ad-3 (6%) and al-i (16%) Between his-3 and nic-2 Between cyh-i (5%) and al-I (7%) Linked to cyh-i (18%), al-i (2%), and mb-2 (6%) Between arg-i (3%) and T(39311) Between tre (41%) and ad-9 Linked to mt (9%) Linked to mt (20%) Complex phenotype. Alternative requirements: 2-acetylaminofluorine, certain azo dyes, or certain single amino acids. Cold sensitive. Originated among progeny of a rib-i strain that had become tolerant to 2-acetylaminofluorine.

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Linkage Data for Group Iv Markers in Neurospora

Cited by 11 publications

References 4 publications

A SURVEY OF NEW MORPHOLOGICAL MUTANTS IN NEUROSPORA CRASSA

A SURVEY OF NEW MORPHOLOGICAL MUTANTS IN NEUROSPORA CRASSA

MODIFIERS OF THE cot GENE IN NEUROSPORA: THE GULLIVER MUTANTS

Chromosomal loci of Neurospora crassa

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