Linkage map of Salmonella typhimurium, edition IV.

Sanderson, Kenneth E.

doi:10.1128/mmbr.36.4.558-586.1972

Cited by 91 publications

(74 citation statements)

References 73 publications

(119 reference statements)

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“…Four of the six phages produced Mu-sensitive strains at frequencies of 10 to 50% among the survivors. Although the exact nature of the mutations to Mu sensitivity has not been determined, in the four strains tested they were 55% linked to his by P1 transduction and therefore might be in genes analogous to the rfb genes of S. typhimurium (8).…”

Section: Resultsmentioning

confidence: 97%

Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae

Bachhuber¹,

Brill²,

Howe³

1976

J Bacteriol

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Klebsiella pneumoniae M5al is naturally resistant to infection by bacteriophage Mu. Mutants of K. pneumoniae sensitive to Mu infection were isolated and found to support both lytic and lysogenic development of Mu. K. pneumoniae lysogens containing a heat-inducible Mu prophage integrated in his were isolated. Strains carrying deletions extending from his into nif were obtained after heat treatment of these lysogens. Such deletions should be useful for determining the map order and cistronic organization of the nif genes.

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Section: Resultsmentioning

confidence: 97%

Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae

Bachhuber¹,

Brill²,

Howe³

1976

J Bacteriol

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“…The ethers found for SH 835 are . Standard gene symbols [18] used: gal = genes for fermentation of D-galactose; his = genes for biosynthesis of L-histidine; rfa = genes for biosynthesis of lipopolysaccharide core; rfb = genes for biosynthesis of the 0 unit of lipopolysaccharide; rfc = gene(s) for the polymerization of 0 units in group B; oafA = gene(s) for 0-acetylation of abequose to produce factor 5; oafR, oajE, oafF = genes for glucosylation of 0 side chain to produce factor 122 (see Fig. 1 The amount of sugar was determined using D-arabinose as an internal standard and the values for 2-acetamido-2-deoxy-~-glucose have been adjusted by using the response factor 2.0 (SH 835) and have not been accounted for SH [22] and S.…”

Section: Methylation Analysesmentioning

confidence: 99%

O‐Acetylation and Glucosylation of Lipopolysaccaride in Hybrids between Salmonella Groups B and C₂

Rudén

Mäkelä

1974

European Journal of Biochemistry

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The structure of the lipopolysaccharide in two hybrid strains formed between a Salmonella donor of 0 group B and a Salmonella recipient of 0 group Cz was analyzed. Both the hybrids had the coredetermining rfa genes from the CZ parent, and the rfb genes, which determine the biosynthesis of the 0-units, from group B. The core was found to be modified by alkali-labile substituents in a way not found in group B and probably typical of group CZ. As expected, the 0 side chain had O-acetylabequose, (0 factor 5) when the group B oafA genes were present, and D-glucose linked to C-4 of D-galactose (0 factor 122) when the group B (or D) oafR,E,F genes were present. Furthermore the terminal D-glucose was 0-acetylated at C-2. The L-rhamnose of the side chain as well as its D-galactose were found in both strains to carry an alkali-labile group apparently corresponding to group CZtype modifications. It was inferred that the genes determining these modifications were not alleles of the known oaf genes of group B. The previous conclusion, that the lipopolysaccharide-modifying genes function independently to modify, e.g. by 0-acetylation or glucosylation, any sort of lipopolysaccharide offered to them, provided this has suitable attachment sites for the substituents, gets further support from these results.In a previous study [l] we analyzed the lipopolysaccharide (0 antigen) of a hybrid between two Salmonella species of different 0 groups. The hybrid had inherited the rfb genes that determine the 0-specific oligosaccharide ("repeating unit") from its B group parent while most of its other genes and therefore its synthetic capacities were derived from the group CZ parent. The lipopolysaccharide of the hybrid was shown to have the lipopolysaccharide core (see Fig

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“…1 . These six enzymes in the above two organisms are encoded by six unlinked genes (26,29). The route of synthesis of UMP is not unique to these two bacteria but rather it appears to be of a universal nature (23).…”

mentioning

confidence: 99%

Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine

Kelln¹,

Kinahan²,

Foltermann³

et al. 1975

J Bacteriol

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The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic pathway and one enzyme of the arginine biosynthetic pathway were assayed from cells grown under a variety of conditions. Cytidine repressed the synthesis of dihydroorotase (encoded by pyrC), dihydroorotate dehydrogenase (encoded by pyrD), and ornithine transcarbamylase (encoded by argI). Moreover, aspartate transcarbamylase (encoded bypyrB) became further derepressed upon cytidine addition, whereas no change occurred in the levels of the last two enzymes (encoded by pyrE and pyrF) of the pyrimidine pathway. Quantitative nucleotide pool determinations have provided evidence that any individual riboor deoxyribonucleoside mono-, di-, or triphosphate of cytosine or uracil is not a repressing metabolite for the pyrimidine biosynthetic enzymes. Other nucleotide derivatives or ratios must be considered.

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Linkage map of Salmonella typhimurium, edition IV.

Cited by 91 publications

References 73 publications

Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae

Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae

O‐Acetylation and Glucosylation of Lipopolysaccaride in Hybrids between Salmonella Groups B and C₂

Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine

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Linkage map of Salmonella typhimurium, edition IV.

Cited by 91 publications

References 73 publications

Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae

Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae

O‐Acetylation and Glucosylation of Lipopolysaccaride in Hybrids between Salmonella Groups B and C2

Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine

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O‐Acetylation and Glucosylation of Lipopolysaccaride in Hybrids between Salmonella Groups B and C₂