Linkage mapping of four chicken calpain genes

Okumura, Fumihiro; Shimogiri, Takeshi; Shinbo, Yoko; Yoshizawa, K.; Kawabe, Kentaro; Mannen, Hideyuki; Okamoto, Susumu; Cheng, Hans H.; Maeda, Yoshizane

doi:10.1111/j.1740-0929.2005.00246.x

Cited by 7 publications

(10 citation statements)

References 18 publications

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“…1997). Okumura et al. (2005) identified four calpain genes ( CAPN1 , CAPN2 , CAPN3 and CAPN1.5 ) expressed ubiquitously in chicken.…”

Section: Introductionmentioning

confidence: 99%

“…Calpain is a ubiquitous cytoplasmic cysteine protease and its activity is absolutely dependent on calcium (Sorimachi et al 1997). Okumura et al (2005) identified four calpain genes (CAPN1, CAPN2, CAPN3 and CAPN1.5) expressed ubiquitously in chicken. Among them CAPN1 requires calcium at micromolar concentrations for activity and, for CAPN2, calcium at milimolar concentrations is needed (Suzuki & Sorimachi 1998).…”

Section: Introductionmentioning

confidence: 99%

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Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fibre and carcass traits in quality chicken populations

Zhang

Liu

Jiang

et al. 2008

J Animal Breeding Genetics

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This study was aimed at investigating the effect of the calpain 1 (CAPN1) gene on carcass and meat quality traits in eight meat-type chicken populations, including five pure lines (developed from Chinese local breeds) and three cross-breeds. Primer pairs for the Coding Sequence (CDS) region in CAPN1 were designed from the chicken genomic sequence database. Polymorphisms were detected by polymerase chain reaction (PCR)-Single Strand Conformation Polymorphism (SSCP) and DNA sequencing. Three single nucleotide polymorphisms (SNP; C2546T, G3535A and C7198A) were detected among individuals in each population. The associations of their haplotypes (H1 = CGA, H2 = CGC, H3 = CAA, H4 = CAC, H5 = TGA and H7 = TAA) with chicken breast muscle fibre and carcass traits were analysed. Results showed that the haplotypes were associated with live weight (LW), carcass weight (CW), breast muscle weight (BMW) and leg muscle weight (LMW) (p < 0.05), and were also related to eviscerated percentage (%EP) and breast muscle fibre density (p < 0.01). H1H3 haplotype was dominant for LW, CW and BMW; H1H5 haplotype was dominant for EP; H3H4 haplotype was dominant for LMW and H1H1 haplotype was dominant for BFD. It was concluded that the CAPN1 gene may be a major gene affecting meat quality traits of chicken or it is linked with the major gene. H1H3, H1H5 and H3H4 were the most advantageous haplotypes for carcass traits whereas H1H1 was the positive haplotype for breast muscle fibre trait.

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“…1997). Okumura et al. (2005) identified four calpain genes ( CAPN1 , CAPN2 , CAPN3 and CAPN1.5 ) expressed ubiquitously in chicken.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fibre and carcass traits in quality chicken populations

Zhang

Liu

Jiang

et al. 2008

J Animal Breeding Genetics

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“…Recently, Okumura et al . (2005) identified polymorphisms in four calpain genes expressed in chicken (µ‐calpain gene, CAPN1 ; m‐calpain gene, CAPN2 ; p94 gene, CAPN3 ; and µ/m‐calpain, CAPN1.5 ), and mapped them using two backcross families.…”

Section: Introductionmentioning

confidence: 99%

“…We also analyzed the genetic constitution, genetic variability, and relation among the chicken populations using these loci. As the CAPN2 and CAPN1.5 are linked with about 20 cM on chicken chromosome 3 (Okumura et al . 2005), genotypes for these loci were treated as haplotype.…”

Section: Introductionmentioning

confidence: 99%

Gene constitution of South‐East Asian native chickens, commercial chickens and jungle fowl using polymorphisms of four calpain genes

Okumura¹,

Shimogiri²,

Kawabe³

et al. 2006

Animal Science Journal

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The gene constitution of polymorphisms of the four calpain genes (µ‐calpain, m‐calpain, p94, and µ/m‐calpain) were analyzed in South‐East Asian native chickens, White Leghorn and Broiler commercial chickens, and Red and Green jungle fowl. Polymorphisms were detected at all loci in chickens and Red jungle fowl, but only for CAPN1 (µ‐calpain gene) in Green jungle fowl. CAPN2 and CAPN1.5 are linked on chicken chromosome 3, and the genotype for these loci were treated as haplotype. Some combinations of calpain loci were tested using principal component analysis, and the best combination (CAPN1, CAPN3, and CAPN1.5) was determined. The proportion of polymorphic loci (Ppoly) and heterozygosity (H̄) were 1.00 and 0.316–0.465 in domestic chickens and red jungle fowl, and 0.33 and 0.137 in Green jungle fowl, respectively. GST values suggested that the degree of subdivision among native chickens was relatively low except for Thailand, which was highest. Pair‐wise FST testing, dendrogram and principal component analysis from the results of calpain loci showed that the four South‐East Asian native and commercial chicken populations were close genetically.

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“…The calpains were discovered because the Z-disks in the muscle strips incubated in a Ca 2+ -containing solution disappeared in the absence of other ultrastructurally detectable changes (Dayton et al, 1981). Four calpain genes (i.e., μ-calpain gene, CAPN1; m-calpain gene, CAPN2; p94 gene, CAPN3; and μ/m-calpain, CAPN1.5) are expressed ubiquitously in chickens (Okumura et al, 2005). Among the calpain family members, CAPN1 degrades myofibrillar proteins under postmortem conditions and appears to be the primary enzyme in the postmortem tenderization process (Koohmaraie, 1992(Koohmaraie, , 1994(Koohmaraie, , 1996.…”

Section: +mentioning

confidence: 99%

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

JingTing¹,

Zhang²,

Shan³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The micromolar calcium-activated neutral protease gene (CAPN1) is a physiological candidate gene for meat tenderness. Four previously identified single nucleotide polymorphism (SNP) markers located within the CAPN1 gene were evaluated for their associations with variation in the meat tenderness of a Chinese indigenous chicken breed, a higher meat quality breed (i.e., Qingyuan partridge chicken), and the commercial Recessive White chicken breed. WarnerBratzler shear force measurements were used to determine tenderness phenotypes for all animals; intramuscular fat (IMF) content and rate of water loss in the breast muscles were also measured. Genotyping was performed by the polymerase chain reaction-ligase detection reaction method. Polymorphisms were identified for all markers, except CAPN1 2546. The frequency of allele T was zero, and allele C was fixed for CAPN1 2546 in the studied populations. The SNP CAPN1 3535 in the CAPN1 gene was significantly associated with tenderness and other meat quality traits, where animals inheriting the AA genotype had smaller shear force values, lower water loss rates, and higher IMF contents. Moreover, H1 (AAA) was the most advantageous haplotype for meat tenderness. The results of this study confirm some previously documented associations. Furthermore, novel associations have been 1393-1403 (2015) identified that, following validation in other populations, could be incorporated into breeding programs to improve meat quality.

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Linkage mapping of four chicken calpain genes

Cited by 7 publications

References 18 publications

Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fibre and carcass traits in quality chicken populations

Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fibre and carcass traits in quality chicken populations

Gene constitution of South‐East Asian native chickens, commercial chickens and jungle fowl using polymorphisms of four calpain genes

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

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