Linking energy metabolism and locomotor variation to osmoregulation in Chinese shrimp Fenneropenaeus chinensis

Li, Jiangtao; Xu, Xiuwen; Li, Wentao; Zhang, Xiumei

doi:10.1016/j.cbpb.2019.05.006

Cited by 10 publications

(11 citation statements)

References 62 publications

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“…Abdominal and pleopod muscles are generally related to locomotion in L. vannamei (Zhang et al, 2007). The tail-flipping response is suppressed during hypoxic conditions due to lower activity of PFK, PK and lactate dehydrogenase (LDH) in abdominal muscles (Li et al, 2018), whereas increased activities of PFK and LDH in abdominal muscles increase the tail-flipping response (Li et al, 2019). The knockdown of the phosphoglyceromutase gene, involved in glycolysis, results in the lower expression of the gene and reduced muscle thickness in Drosophila melanogaster embryos.…”

Section: Introductionmentioning

confidence: 99%

Silencing of the phosphofructokinase gene impairs glycolysis and causes abnormal locomotion in the subterranean termite Reticulitermes chinensis Snyder

Hassan

Huang

et al. 2020

Insect Molecular Biology

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Phosphofructokinase (PFK) is a rate‐limiting enzyme in glycolysis, but its linkage with locomotion in termites is not well understood, despite the demonstrated involvement of this gene in the locomotion of different animals. Here, we investigated the effect of the pfk gene on locomotion in the subterranean termite Reticulitermes chinensis Snyder through RNA interference and the use of an Ethovision XT tracking system. The knockdown of pfk resulted in significantly decreased expression of the pfk gene in different castes of termites. The pfk‐silenced workers displayed higher levels of glucose but lower levels of nicotinamide adenine dinucleotide (NADH) and adenosine triphosphate (ATP) production and decreased activity of the PFK enzyme. Furthermore, abnormal locomotion (decreased distance travelled, velocity and acceleration but increased turn angle, angular velocity and meander) was observed in different castes of pfk‐silenced termites. We found caste‐specific locomotion among workers, soldiers and dealates. Additionally, soldiers and dealates showed higher velocity in the inner zone than in the wall zone, which is considered an effective behaviour to avoid predation. These findings reveal the close linkage between the pfk gene and locomotion in termites, which helps us to better understand the regulatory mechanism and caste specificity of social behaviours in social insects.

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Section: Introductionmentioning

confidence: 99%

Silencing of the phosphofructokinase gene impairs glycolysis and causes abnormal locomotion in the subterranean termite Reticulitermes chinensis Snyder

Hassan

Huang

et al. 2020

Insect Molecular Biology

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“…In the study, we expected that the allostatic load on F. chinensis would increase when salinity decreased from 20 ppt to 5 ppt. All forms of allostasis require energy, as allostatic load increases, the amount of energy available for other biological functions decreases [6, 21, 23, 25, 55]. We found that most of the DEGs in the ribosome pathway (ko03010) were downregulated in the LS group as compared to the control (Table 5).…”

Section: Discussionmentioning

confidence: 82%

“…On this point, the results support the opinion reviewed by Péqueux (1995) and Henry (2012) that osmoregulators in low-salinity environment (below 26 ppt) will turn on mechanisms of anisomotic extracelluar regulation to stabilize hemolymph osmotic and ionic concentrations [1, 3]. Although the control group (20 ppt) is already in low-salinity environment according to reference salinity of 26 ppt, the result has implied that the salinity difference between 5 ppt and 20 ppt is too huge enough to make their gene expression difference, as well as the hemolymph osmolality [21].…”

Section: Discussionmentioning

confidence: 99%

“…In crustaceans similarly exposed to salinity stress (e.g., the swimming crab, Portunus trituberculatus; the Chinese crab, Eriocheir sinensis; and the Pacific white shrimp, Penaeus vannamei ), several genes in pathways potentially associated with adaptations to low salinity have been reported [22–25]. Reports showed that F. chinensis has a poorer ability to maintain a stable hemolymph osmolarity (reflecting the weaker low-salinity adaptation), compared to P. vannamei [21, 26]. The poorer ability limits the farming development of F. chinensis .…”

Section: Introductionmentioning

confidence: 99%

“…However, to date, studies on low-salinity adaptation in F. chinensis are still lacking on molecules. Recently Li (2019b) reported on F. chinensis that the group after exposure at low salinity (10 ppt) showed significantly elevated citrate synthase (CS) and cytochrome C oxidase (COX) activities in its gill when compared with the group subjected to 20 ppt salinity condition [21]. For this species, these proteins have also included Na + /K + -ATPase, phenoloxidase (PO), heat shock proteins (HSPs), ion-transport enzymes [27–29].…”

Section: Introductionmentioning

confidence: 99%

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Unraveling Molecular Mechanisms of Low-Salinity Adaptation inFenneropenaeus chinensis: Insights from Gill Tissue Transcriptomics

Liu¹,

Zhang²,

Wang

et al. 2020

Preprint

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Ability to tolerate low salinity is a key factor affecting the distribution of the Chinese shrimp (Fenneropenaeus chinensis). Although previous studies have investigated the mechanisms underlying adaptations to low salinity in some crustaceans, little is known about low-salinity adaptations in F. chinensis, particularly at the molecular level. Here, to identify genes potentially associated with the molecular response of F. chinensis to low-salinity exposure, we compared the transcriptomes of F. chinensis in low-salinity (5 ppt) and normal-salinity (20 ppt) environments. In total, 45,297,936 and 44,685,728 clean reads were acquired from the low-salinity and control groups, respectively. De novo assembly of the clean reads yielded 159,130 unigenes, with an average length of 662.82 bp. Of these unigenes, only a small fraction (10.5% on average) were successfully annotated against six databases. We identified 3,658 differentially expressed genes (DEGs) between the low-salinity group and the control group: 1,755 DEGs were downregulated in the low-salinity group as compared to the control, and 1,903 were upregulated. Of these DEGs, 282 were significantly overrepresented in 38 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways. Notably, several DEGs were associated with pathways important for osmoregulation, including the mineral absorption pathway (ATP1A, Sodium/potassium-transporting ATPase subunit alpha; CLCN2, Chloride channel 2; HMOX2, Heme oxygenase 2; SLC40A1/FPN1, Solute carrier family 40 iron-regulated transporter, member 1), the vasopressin-regulated water reabsorption pathway (AQP4, Aquaporin-4; VAMP2, Vesicle-associated membrane protein 2; RAB5, Ras-related protein Rab-5) and the ribosome pathway. Our results help to clarify the molecular basis of low-salinity adaptations in F. chinensis.

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Effect of abamectin on osmoregulation in red swamp crayfish (Procambarus clarkii)

Tianyu,

QianQian,

Jingyuan

et al. 2024

Environ Sci Pollut Res

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Linking energy metabolism and locomotor variation to osmoregulation in Chinese shrimp Fenneropenaeus chinensis

Cited by 10 publications

References 62 publications

Silencing of the phosphofructokinase gene impairs glycolysis and causes abnormal locomotion in the subterranean termite Reticulitermes chinensis Snyder

Silencing of the phosphofructokinase gene impairs glycolysis and causes abnormal locomotion in the subterranean termite Reticulitermes chinensis Snyder

Unraveling Molecular Mechanisms of Low-Salinity Adaptation inFenneropenaeus chinensis: Insights from Gill Tissue Transcriptomics

Effect of abamectin on osmoregulation in red swamp crayfish (Procambarus clarkii)

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