Linking fluorescence induction curve and biomass in herbicide screening

Abstract

A suite of dose-response bioassays with white mustard (Sinapis alba L) and sugar beet (Beta vulgaris L) in the greenhouse and with three herbicides was used to analyse how the fluorescence induction curves (Kautsky curves) were affected by the herbicides. Bentazone, a photosystem II (PSII) inhibitor, completely blocked the normal fluorescence decay after the P-step. In contrast, fluorescence decay was still obvious for flurochloridone, a PDS inhibitor, and glyphosate, an EPSP inhibitor, which indicated that PSII inhibition was incomplete. From the numerous parameters that can be derived from OJIP-steps of the Kautsky curve the relative changes at the J-step [Fvj = (Fm - Fj)/Fm] was selected to be a common response parameter for the herbicides and yielded consistent dose-response relationships. Four hours after treatment, the response Fvj on the doses of bentazone and flurochloridone could be measured. For glyphosate, the changes of the Kautsky curve could similarly be detected 4 h after treatment in sugar beet, but only after 24 hs in S alba. The best prediction of biomass in relation to Fvj was found for bentazone. The experiments were conducted between May and August 2002 and showed that the ambient temperature and solar radiation in the greenhouse could affect dose-response relationships. If the Kautsky curve parameters should be used to predict the outcome of herbicide screening experiments in the greenhouse, where ambient radiation and temperature can only partly be controlled, it is imperative that the chosen fluorescence parameters can be used to predict accurately the resulting biomass used in classical bioassays.

Metabolic response of cornflower (Centaurea cyanus L.) exposed to tribenuron-methyl: one of the active substances of sulfonylurea herbicides

“…Raman spectroscopy is used in studying plant responses to stress factors, which makes it possible to track stress-induced changes in the chemical composition of tissues (Skoczowski and Troć 2013). Fluorescence methods have been used in the studies on the effects of herbicides on plants (Christensen et al 2003) due to the quick, non-invasive and simple manner of assessing the physiological state of plants in comparison with traditional methods of resistance testing (Beckie et al 2000).…”

The Effect of Sugar Beet Broadleaf Herbicides on Fluorescence Induction Curves in Amaranthus retroflexus L. and Portulaca oleracea L.

“…Finally, cell membranes and tissues disintegrate due electron transport chain reaction of free-radical attack (Cobb and Reade, 2010). This phenomenon was observed in case of many PSII inhibitors by a large number of researchers (Habash et al, 1985;Van Heerden et al, 2003;Christensen et al, 2003;Abbaspoor et al, 2006;Chitband et al, 2015;Thach and Truong, 2016). These transformations in the shape of the Kautsky curve were irreversibly affected by the PSII inhibitor (Fig.…”

“…These phases primarily indicate photochemical events relevant to PSII (Govindjee, 1995). Three phases are found discovered on the O, J, I and P steps and depicted as follows: The O-J phase, complete reduction of the primary electron acceptor QA of PSII addition, photosynthesis is not the primary target site of the growth regulator herbicides (PGR) such as clopyralid and ethofumesate as fatty acid biosynthesis inhibitors, but changes in chlorophyll fluorescence induction curve responses were formerly observed in treated plants (Percival and Baker, 1991;Christensen et al, 2003;Abbaspoor and Streibig, 2005). Barbagallo et al (2003) found early effects on the shape of the Kautsky curve and derived fluorescence parameters of Arabidopsis thaliana seedlings by asulam, bifenox, 2,4-D, diclofop-methyl, glyphosate and imazapyr which do not have a direct impact on photosynthesis.…”

Structure‐based design of novel Chlamydomonas reinhardtii D1‐D2 photosynthetic proteins for herbicide monitoring

“…41,42 From the shape and the form of the fluorescence transition curve (or Kautsky curve), in fact, it is possible to derive various fluorescence parameters that provide information on the photochemical efficiency of PSII. The fluorescence parameter 1ÀV J was used to determine the herbicide effect, and it was calculated by the following equation 28 :…”