Linking protein oxidation to environmental pollutants: Redox proteomic approaches

Braconi, Daniela; Bernardini, Giulia; Santucci, Annalisa

doi:10.1016/j.jprot.2011.06.029

Cited by 74 publications

(29 citation statements)

References 149 publications

(171 reference statements)

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“…Notably, the peroxidation of membrane (phospho) lipids and the degradation/oxidation of proteins are among the most investigated consequences of ROS action on membrane structure and function (Blokhina et al 2003;Davies 2005;Rinalducci et al 2008;Foyer and Noctor 2009;Foyer and Shigeoka 2011). Regarding the interaction of ROS with lipids and proteins, superoxides (O 2 •− ) react primarily with protein Fe-S centers, while singlet oxygen ( 1 O 2 ) is particularly prone to react with conjugated double bonds such as those found in polyunsaturated fatty acids (PUFAs) (Rinalducci et al 2008; reviewed by Braconi et al 2011). Enhanced protein oxidation and lipid peroxidation may take place in membranes of the cell and the organelle, which, in turn, can affect the normal cellular functioning and metabolism.…”

Section: Introductionmentioning

confidence: 99%

Lipids and proteins—major targets of oxidative modifications in abiotic stressed plants

Anjum

Sofo

Scopa

et al. 2014

Environ Sci Pollut Res

283

160

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Stress factors provoke enhanced production of reactive oxygen species (ROS) in plants. ROS that escape antioxidant-mediated scavenging/detoxification react with biomolecules such as cellular lipids and proteins and cause irreversible damage to the structure of these molecules, initiate their oxidation, and subsequently inactivate key cellular functions. The lipid- and protein-oxidation products are considered as the significant oxidative stress biomarkers in stressed plants. Also, there exists an abundance of information on the abiotic stress-mediated elevations in the generation of ROS, and the modulation of lipid and protein oxidation in abiotic stressed plants. However, the available literature reflects a wide information gap on the mechanisms underlying lipid- and protein-oxidation processes, major techniques for the determination of lipid- and protein-oxidation products, and on critical cross-talks among these aspects. Based on recent reports, this article (a) introduces ROS and highlights their relationship with abiotic stress-caused consequences in crop plants, (b) examines critically the various physiological/biochemical aspects of oxidative damage to lipids (membrane lipids) and proteins in stressed crop plants, (c) summarizes the principles of current technologies used to evaluate the extent of lipid and protein oxidation, (d) synthesizes major outcomes of studies on lipid and protein oxidation in plants under abiotic stress, and finally, (e) considers a brief cross-talk on the ROS-accrued lipid and protein oxidation, pointing to the aspects unexplored so far.

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Section: Introductionmentioning

confidence: 99%

Lipids and proteins—major targets of oxidative modifications in abiotic stressed plants

Anjum

Sofo

Scopa

et al. 2014

Environ Sci Pollut Res

283

160

View full text Add to dashboard Cite

show abstract

“…Vacuolar compartmentation of heavy metal is one of the cell's various methods of coping with the toxic effects of ROS caused by metal exposure. This strategy can mitigate the oxidative stress that leading to lipid peroxidation, biological macromolecule descent, membrane ruination, ion leakage, DNA-strand cleavage, and eventually death of plants (Romero-Puertas et al 2002;Sharma and Dubey 2007;Braconi et al 2011;Rascio and NavariIzzo 2011;Hossain et al 2012;. A similar mechanism may be involved in metal hyperaccumulation and tolerance of CsHMA3 transgenic Camelina plants.…”

Section: Discussionmentioning

confidence: 99%

Changes in fatty acid content and composition between wild type and CsHMA3 overexpressing Camelina sativa under heavy-metal stress

et al. 2015

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Under heavy-metal stress, CsHMA3 overexpressing transgenic Camelina plants displayed not only a better quality, but also a higher quantity of unsaturated fatty acids in their seeds compared with wild type. Camelina sativa L. belongs to the Brassicaceae family and is frequently used as a natural vegetable oil source, as its seeds contain a high content of fatty acids. In this study, we observed that, when subjected to heavy metals (Cd, Co, Zn and Pb), the seeds of CsHMA3 (Heavy-Metal P1B-ATPase 3) transgenic lines retained their original golden yellow color and smooth outline, unlike wild-type seeds. Furthermore, we investigated the fatty acids content and composition of wild type and CsHMA3 transgenic lines after heavy metal treatments compared to the control. The results showed higher total fatty acid amounts in seeds of CsHMA3 transgenic lines compared with those in wild-type seeds under heavy-metal stresses. In addition, the compositions of unsaturated fatty acids-especially 18:1 (oleic acid), 18:2 (linoleic acid; only in case of Co treatment), 18:3 (linolenic acid) and 20:1 (eicosenoic acid)-in CsHMA3 overexpressing transgenic lines treated with heavy metals were higher than those of wild-type seeds under the same conditions. Furthermore, reactive oxygen species (ROS) contents in wild-type leaves and roots when treated with heavy metal were higher than in CsHMA3 overexpressing transgenic lines. These results indicate that overexpression of CsHMA3 affects fatty acid composition and content-factors that are responsible for the fuel properties of biodiesel-and can alleviate ROS accumulation caused by heavy-metal stresses in Camelina. Due to these factors, we propose that CsHMA3 transgenic Camelina can be used for phytoremediation of metal-contaminated soil as well as for oil production.

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“…135 Recently, oxidative stress is identified as a common mechanism of action for EDC in affecting cellular structures and functions. 136 Induction of oxidative stress was detected in epididymal sperms of rats, exposed to BPA. 137 Specifically EDC-induced oxidative stress caused disruption to tight and adherens junctions between Sertoli-Sertoli cells and Sertoli-germ cells.…”

Section: Effects Of Edc On Adult Spermatogenesismentioning

confidence: 97%

Endocrine disrupting chemicals

et al. 2011

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Linking protein oxidation to environmental pollutants: Redox proteomic approaches

Cited by 74 publications

References 149 publications

Lipids and proteins—major targets of oxidative modifications in abiotic stressed plants

Lipids and proteins—major targets of oxidative modifications in abiotic stressed plants

Changes in fatty acid content and composition between wild type and CsHMA3 overexpressing Camelina sativa under heavy-metal stress

Endocrine disrupting chemicals

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