Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Abstract

ABSTRACT:The activity of lipase in fermented Africa locust bean, Castor seed, and Africa oil bean was determined. The peak lipase activity for fermented Africa locust bean, Castor seed, and African oil bean were 13.3x10 -3 µmol/sec, 7.2 x10 -3 µmol/sec and 10.6 x10 -3 µmol/sec at day 5, 4 and 4 respectively. The optimum temperature and pH were 30 0 C and 7.0 respectively for all the fermented seeds above. Increasing NaCl concentration decreases the activity of Lipase indicating that NaCl is an inhibitor of lipase. The effect of substrate concentration on lipase activity in the fermenting oil seeds shows the normal Michaelis-Menten curve. The K m for African locust bean is 0.065664mM, Castor seed is 0.067625mM, and African oil bean is 0.075848mM. While the V max are: 15.229x10 -3 µmol/sec, 14.787x10 -3 µmol/sec and 13.184x10 -3 µmol/sec respectively. The findings of this work indicates that fermented African locust bean, Castor seed and Africa oil bean could be a source of lipase for industrial application.

Assessment of Biochemical and Antimicrobial Activity of Bacteria Associated with Fermented Locust Beans in Manyare Community of Kogi State, Nigeria

“…It is expected that the longer the period of fermentation (4-5 days), the better the product, due to more activities of the fermenting bacterial flora, but period of fermentation is another major difference in the method of traditional production of Iru, because nowadays most of the fermentation times are usually one to three days instead of four to five days. The result obtained in this research corroborates with the study of Liman et al (2010) who demonstrated the impacts of some environmental conditions on quality of processed locust beans.…”

“…It is deciduous perennial tree that grows to between 7 and 20 m high. In some cases, up to 30 m (Ntui et al, 2012). The tree is a fire-resistant helophyte characterized by a thick dark gray-brown bark (Abioye et al, 2013).…”

Postharvest processing, packaging and storage of African oil bean seed

“…A work by [8] posits that the seed is a good source of lipase. AOB seed improves metabolism and possess anti-atherogenic property [9].…”

LIPASE ACTIVITIY OF MIXTURE OF FERMENTED AVOCADO (Persea americana), BANANA (Moses paradisiaca) AND SNAKEFRUIT (Salacca zalacca)

“…Research Liman et al, (2010), found that lipase from Africa locust bean, Castor seed, and Africa oil bean has an optimum pH 7. Similarly, Pratiwi et al, (2013) …”