Lipase-Ca2+ hybrid nanobiocatalysts through interfacial protein-inorganic self-assembly in deep-eutectic solvents (DES)/water two-phase system for biodiesel production

Construction of Immobilized Enzymes with Yeast and Metal–Organic Frameworks for Enhanced Biocatalytic Activities

“…13 The choice of immobilization support is a critical factor as it affects the overall efficiency, feasibility, and cost of the enzyme system. 11 Conventionally, biopolymers, 14 silica sol−gels, 15 mesoporous silica, 8,16,17 and carbon nanomaterials 18 have been used as support materials for enzyme immobilization. In recent years, a new type of porous material, namely metal− organic frameworks (MOFs), has also been widely investigated for enzyme immobilization.…”

“…Meanwhile, enzyme immobilization facilitates product purification while reducing process costs . Therefore, compared with free enzymes, immobilized enzymes have a series of advantages, such as high storage stability, high reusabilities, high tolerance to harsh environments, easier recovery, continuous and controlled operation, as well as simplicity in process, while maintaining their efficient and mild enzymatic catalytic properties. However, immobilized enzymes also have some disadvantages, such as no protection on the surface of the enzyme after immobilization and restriction by hydrophobicity of the carrier and enzyme …”

“…The choice of immobilization support is a critical factor as it affects the overall efficiency, feasibility, and cost of the enzyme system . Conventionally, biopolymers, silica sol–gels, mesoporous silica, ,, and carbon nanomaterials have been used as support materials for enzyme immobilization. In recent years, a new type of porous material, namely metal–organic frameworks (MOFs), has also been widely investigated for enzyme immobilization. − MOFs are one-, two-, or three-dimensional structured compounds formed by metal ions (or clusters) being coordinated with varied organic molecular ligands. , By changing the central metal ions (or clusters) and ligands, different types of MOFs can be easily synthesized .…”

“…However, immobilized enzymes also have some disadvantages, such as no protection on the surface of the enzyme after immobilization 12 and restriction by hydrophobicity of the carrier and enzyme. 13 The choice of immobilization support is a critical factor as it affects the overall efficiency, feasibility, and cost of the enzyme system. 11 Conventionally, biopolymers, 14 silica sol−gels, 15 mesoporous silica, 8,16,17 and carbon nanomaterials 18 have been used as support materials for enzyme immobilization.…”

Chitosan‐Based metal-organic framework for Stabilization of β-glucosidase: Reusability and storage stability

“…To address these limitations, recent studies have been focused on enzyme-shielding strategies. For example, researchers have successfully shielded immobilized lipase on silica nanospheres by applying a porous silica layer through silane self-assembly and polycondensation, effectively protecting the enzyme from protease degradation [ 14 ].…”