Lipase catalysed biodiesel synthesis with integrated glycerol separation in continuously operated microchips connected in series

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

“…Salis et al [39] found that C. antarctica lipase B was progressively inactivated by methanol in amounts above 1/2 molar equivalent. In the work of Šalić et al (2018) [40] commercial lipase from T. lanuginosus was completely deactivated by methanol after 25 h using higher amounts of methanol. However, the purified enzyme in this study followed the same deactivation trend despite the fact that a lower oil to methanol ratio was used.…”

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

“…36 Worth to mention is also the recently published special issues devoted to microreactors in biotechnology. [37][38][39][40][41][42] Additionally, a very recent review article was published by Hughes dealing with applications of flow chemistry in the pharmaceutical industry focusing in the patent literature, 43 whereas Lee et al emphasized on the translation of the pharmaceutical manufacturing from batch to flow, 44 and Baumann et al concentrate on the perspectives of continuous flow chemistry in the pharmaceutical industry. 45 Monbaliu and co-workers summarize the impact of continuous flow on platform-chemicals derived from biomass.…”

The Promises and the Challenges of Biotransformations in Microflow

“…The increased use of biocatalytic steps in industrial-scale organic synthesis, particularly for the synthesis of active Table 1. L) Biodiesel production by transesterification of edible sunflower and waste cooking oil [65] Straightforward capacity increase Bespoke two-plate packed-bed reactor ω-Transaminase from metagenomic library entrapped in LentiKats…”

“…[64] Integration of microreactors with downstream processing can further lead to ideal end-to-end synthesis. [98,99] Examples of enzymatic microreactors coupled with separation units comprise miniaturized membranes, [38,39] also with the application of an electric field, [56] extractors, [56,63,65] and evaporators. [63,77] Integrated lipase-catalyzed biodiesel production was performed in a system of a capillary microreactor providing a stable emulsion of aqueous enzyme solution in oil and further connected with liquid-liquid microseparator for simultaneous glycerol separation using DES.…”

“…[63,77] Integrated lipase-catalyzed biodiesel production was performed in a system of a capillary microreactor providing a stable emulsion of aqueous enzyme solution in oil and further connected with liquid-liquid microseparator for simultaneous glycerol separation using DES. [65] Another approach was an inline purification step based on the sequential reaction of the unreacted substrate directly in a polymer-supported resin. [31] Moreover, a highly innovative integrated continuous process was recently reported comprising two-step enzymatic reaction employing transaminases and redox enzymes enabling cofactor recycling and further recirculation after inline recovery of benign by-products by liquid-liquid extraction.…”