Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

Abstract

Biodiesel is an alternative diesel fuel made from renewable biological resources. During the process of biodiesel production, lipase-catalyzed transesterification is a crucial step. However, current techniques using methanol as acyl acceptor have lower enzymatic activity; this limits the application of such techniques in large-scale biodiesel production. Furthermore, the lipid feedstock of currently available techniques is limited. In this paper, the technique of lipase-catalyzed transesterification of five different oils for biodiesel production with methyl acetate as acyl acceptor was investigated, and the transesterification reaction conditions were optimized. The operation stability of lipase under the obtained optimal conditions was further examined. The results showed that under optimal transesterification conditions, both plant oils and animal fats led to high yields of methyl ester: cotton-seed oil, 98%; rapeseed oil, 95%; soybean oil, 91%; tea-seed oil, 92%; and lard, 95%. Crude and refined cottonseed oil or lard made no significant difference in yields of methyl ester. No loss of enzymatic activity was detected for lipase after being repeatedly used for 40 cycles (ca. 800 h), which indicates that the operational stability of lipase was fairly good under these conditions. Our results suggest that cotton-seed oil, rape-seed oil and lard might substitute soybean oil as suitable lipid feedstock for biodiesel production. Our results also show that our technique is fit for various lipid feedstocks both from plants and animals, and presents a very promising way for the large-scale biodiesel production.

Enzymatic Production of Biodiesel from Microalgal Oil using Ethyl Acetate as an Acyl Acceptor

“…Accordingly, 40 was selected as the optimum temperature. The optimum temperatures of 50 and 40 have also been previously reported to achieve FAEE yields of 92.7 and 91.0 , respectively from the transesterification of sunflower oil and soybean oil in the presence of ethyl acetate and methyl acetate when using Novozym 435 18,23 .…”

Biodiesel at the Crossroads: A Critical Review

“…These studies manifested that the reaction conditions to obtain Gliperol are similar to that for the production of Biodiesel. Likewise, this reaction has been studied over heterogeneous acid catalysts [68][69][70][71][72][73][74][75][76][77][78][79] as well as using lipases as biocatalysts, either in solvent-free systems [80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95][96][97] or in ionic liquids [98][99][100][101]. Furthermore, many other innovative technologies have also been applied, such as supercritical conditions [102][103][104][105][106][107][108][109][110][111][112][113][114][115]…”

Synthesis Biodiesel from Palm Oil Through Interesterification Using Imobilized Lipase Enzym as Catalyst: The Effect of Amount of Biocatalyst, Mole Ratio of Reactan, Temperature to Yield

“…As reported that if methyl acetate is over it make the oil is more liquid cause the declining of conversion from methyl ester. [17] Mole Figure 6 shows that the increasing of number of biocatalyst has a significant influence to the %yield with fixed variable of mole ratio of reactant 1 : 6. But it is not same to the temperature without a significant influence to %yield.…”