Lipase-catalyzed ethanolysis for biodiesel production of untreated palm oil mill effluent

Rachmadona, Nova; Amoah, Jerome; Quayson, Emmanuel; Hama, Shinji; Yoshida, Ayumi; Kondo, Akihiko; Ogino, Chiaki

doi:10.1039/c9se00457b

Cited by 25 publications

(13 citation statements)

References 21 publications

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“…This reaction, however, was far from meeting the FAAE standard for biodiesel synthesis, which is 96.5% w/w. Optimization of biodiesel from POME was accomplished in our previous research using a lower concentration of ethanol 34 . Based on the results of our previous research, r‐CALB was used in a comparative study with Novozym 435 (a commercially available hydrophobic acrylic resin‐immobilized CALB) to ascertain its effectiveness in catalyzing the ethanolysis of POME (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The total organic carbon and total carbon in the POME was analyzed using a total organic carbon analyzer (TOC‐L, Shimadzu Corp., Tokyo, Japan). A functional group comparison between commercial biodiesel and POME biodiesel was characterized by Attenuated total reflection‐Fourier‐transform infrared spectroscopy (ATR‐FTIR) analysis using a Shimadzu AIM‐900 Infrared Microscope equipped with an ITRraces‐100 (Shimadzu Corp., Tokyo, Japan) 34 …”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, POME could also be used as a sustainable feedstock for biodiesel. In our previous research, the utilization of untreated POME as raw material for biodiesel was established 34,35 . Ultimately, the present study aimed not only to investigate the simultaneous cultivation and immobilization of Aspergillus oryzae expressing various lipases using POME as a carbon source but also to determine the utilization and valorization of untreated POME for biodiesel using this recommended system for whole‐cell lipase‐immobilization with low ethanol concentration.…”

Section: Introductionmentioning

confidence: 99%

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Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis

Rachmadona

Quayson

Amoah

et al. 2021

Biofuels Bioprod Bioref

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Palm oil mill effluent (POME) is a palm industrial waste by‐product that is readily available and possesses high quantities of the organic compounds that are necessary for microbial growth. This study investigated the potential of immobilized Aspergillus oryzae whole cells expressing Candida antartica lipase B (r‐CALB) when using a POME‐based medium as a low‐cost carbon source within biomass support particles. The dry cell weight, hydrolytic activity, and remaining protein were measured for a variety of POME (0–2% w/v) and carbon source concentrations (0–2% w/v) with an incubation time of 96 h. Using POME in the culture medium as a carbon source instead of glucose doubled the retention of cell weight from 1.16 ± 0.03 to 2.36 ± 0.02 g within the biomass support particles and improved the hydrolytic activity from 1.61 ± 0.02 to 2.23 ± 0.02 U mg−1 in whole‐cell lipase. This immobilized r‐CALB was used as a catalyst to esterify POME into biodiesel. This synthesis produced an ester content of 97.52 ± 0.21% w/w after 96 h. Immobilized r‐CALB was reusable for more than ten batches, and resulted in an ethyl ester content of more than 90% w/w even after 10 cycles. The present study demonstrated that POME could serve as a sustainable carbon source for industrial microbial culture with the additional incentive of being a treatment that is inexpensive and sustainable. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis

Rachmadona

Quayson

Amoah

et al. 2021

Biofuels Bioprod Bioref

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“…A thermo-stable lipase has capabilities to catalyzing in bioenergy, pharmaceutical manufacturing and for trans-esterification of palm oil to FAMEs also resistant to organic solvents obtained from Acinetobacter baylyi [ 553 , 554 ]. Furthermore, lipases are also applicable for the curing of hair loss and skin scalp disease [ 555 ].…”

Section: Industrial Applications Of Lipasesmentioning

confidence: 99%

Microbial lipases and their industrial applications: a comprehensive review

et al. 2020

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Lipases are very versatile enzymes, and produced the attention of the several industrial processes. Lipase can be achieved from several sources, animal, vegetable, and microbiological. The uses of microbial lipase market is estimated to be USD 425.0 Million in 2018 and it is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018. Microbial lipases (EC 3.1.1.3) catalyze the hydrolysis of long chain triglycerides. The microbial origins of lipase enzymes are logically dynamic and proficient also have an extensive range of industrial uses with the manufacturing of altered molecules. The unique lipase (triacylglycerol acyl hydrolase) enzymes catalyzed the hydrolysis, esterification and alcoholysis reactions. Immobilization has made the use of microbial lipases accomplish its best performance and hence suitable for several reactions and need to enhance aroma to the immobilization processes. Immobilized enzymes depend on the immobilization technique and the carrier type. The choice of the carrier concerns usually the biocompatibility, chemical and thermal stability, and insolubility under reaction conditions, capability of easy rejuvenation and reusability, as well as cost proficiency. Bacillus spp., Achromobacter spp., Alcaligenes spp., Arthrobacter spp., Pseudomonos spp., of bacteria and Penicillium spp., Fusarium spp., Aspergillus spp., of fungi are screened large scale for lipase production. Lipases as multipurpose biological catalyst has given a favorable vision in meeting the needs for several industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. This review represents a discussion on microbial sources of lipases, immobilization methods increased productivity at market profitability and reduce logistical liability on the environment and user.

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“…Within forming functional groups of biodiesel fuel, to analyze total-Fourier-transform infrared spectroscoi (atr-ftir) is by using the infrared microscope of Shimadzu aim-900 with itrraces-100 (Shimadzu corp., Tokyo, Japan) [7].…”

Section: Analysis Of Biodieselmentioning

confidence: 99%

Production of Biodiesel From Unrefined Plants-Derived Oil by Aspergillus oryzae Expressing Bacillus thermocatenulatus Lipase as a Whole-Cell Biocatalyst

Rachmadona¹,

Aznury²,

Ogino³

2021

Atlantis Highlights in Engineering

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In this study, the enzymatic process reaction using a whole-cell biocatalyst was done for biodiesel production of crude plant oils. This work investigated a streamlined technique to convert crude palm oil that consisted high amount of phosphocholine gums. This reaction by using lipase as a catalyst for biodiesel-fuel has an excellent potential compared with chemical reactions. We explored Aspergillus oryzae expressing Bacillus thermocatenulatus lipase (BTL) as a biocatalyst in producing fatty acid methyl FAME (95.54%) obtained at 30 o C, with 5.00 % (w/w) of BTL at 96 h using four molar equivalents of methanol. Therefore, BTL could be useful as whole-cell biocatalyst that can produce biodiesel from feedstock with a high proportion of impurities in industrial application.

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Lipase-catalyzed ethanolysis for biodiesel production of untreated palm oil mill effluent

Abstract: Scheme of palm oil mill effluent (POME) as a feedstock for biodiesel production.

Cited by 25 publications

References 21 publications

Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis

Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis

Microbial lipases and their industrial applications: a comprehensive review

Production of Biodiesel From Unrefined Plants-Derived Oil by Aspergillus oryzae Expressing Bacillus thermocatenulatus Lipase as a Whole-Cell Biocatalyst

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