Lipase catalyzed glycerolysis of ratfish liver oil at stirred tank basket reactor: A kinetic approach

Arranz-Martínez, Pablo; Corzo‐Martínez, Marta; Vázquez, Luís; Reglero, Guillermo; Torres, Carlos F.

doi:10.1016/j.procbio.2017.09.026

Cited by 15 publications

(8 citation statements)

References 32 publications

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“…In this sense, this work presented the Ping-Pong Bi-Bi mathematical model described by the set of equations -Eq. ( 1) to Eq (6), and also the hydrolysis reactions, characterized by equations Eq (7) to Eq (10).…”

Section: Kinetic Modelingmentioning

confidence: 99%

Kinetic study of liquid lipase-catalyzed glycerolysis of olive oil using Lipozyme TL 100L

Finco

Fiametti

Salva

et al. 2021

Preprint

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Monoacylglycerol (MAG) and diacylglycerol (DAG) are two natural components found in most edible oils and fats. Conventional synthesis of MAG and DAG is usually conducted by glycerolysis of triacylglycerol (TAG) at high temperatures (above 200 °C) in the presence of an alkaline catalyst. In this work, the synthesis of MAG and DAG using enzymatic glycerolysis of olive oil was investigated using Tween 80 as surfactant, n-butanol as co-surfactant and the novel lipase in free/liquid formulation Lipozyme TL 100L as catalyst. Experimental design was used to evaluate the effect of enzyme load and reaction temperature on the feedstock conversion. Enzyme load and system temperature were significant variables in the statistical design and the best condition was found at 35 °C, 7.5 vol% of Lipozyme TL 100 and glycerol to oil molar ratio of 2:1 with conversion of TAG at approximately 98 % after 2 h of process. A mathematical model based on the Ping-Pong Bi-Bi mechanism was used to describe the reaction kinetics. The model adequately described the behavior of the system and can be a useful tool for the design of reactors in larger scales.

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Section: Kinetic Modelingmentioning

confidence: 99%

Kinetic study of liquid lipase-catalyzed glycerolysis of olive oil using Lipozyme TL 100L

Finco

Fiametti

Salva

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The simplest model reduces the reactions to a pseudo-first-order kinetic approximation, generally the model of choice for most kinetic fittings. ,,, It is assumed that the catalyzed reactions proceed sufficiently to completion that they may be considered irreversible. Vinyl acetate is considered to be present in significant excess, and the enzyme activation reaction is considered to reach equilibrium extremely rapidly.…”

Section: Modelingmentioning

confidence: 99%

“…7,8 For example, Oliveira et al, 9 in an attempt to model a transesterification reaction in scCO 2 , simplified their ping− pong mechanism to four parameters and then to just two-and three-parameter models, specifically citing overfitting concerns. Arranz-Martı ́nez et al 10 similarly reduced their eight-step reaction network to just three parameters, also citing overparameterization. Ciftci and Temelli 11 used a semiempirical two-parameter model to model their synthesis of biodiesel.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Modeling of a Consecutive Enzyme-Catalyzed Enantioselective Reaction in Supercritical Media

2020

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Based on experimental data of both batch and continuous enzyme-catalyzed kinetic resolutions of (±)- trans -1,2-cyclohexanediol in supercritical carbon dioxide, kinetic models of increasing complexity were developed to explore the strengths and drawbacks of various modeling approaches. The simplest, first-order model proved to be a good fit for the batch experimental data in regions of high reagent concentrations but failed elsewhere. A more complex system that closely follows the true mechanism was able to fit the full range of experimental data, find constant reaction rate coefficients, and was successfully used to predict the results of the same reaction run continuously in a packed bed reactor. Care must be taken when working with such models, however, to avoid problems of overfitting; a more complex model is not always more accurate. This work may serve as an example for more rigorous reaction modeling and reactor design in the future.

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“…For this, the area of contact between enzymes and substrate is increased with the use of micelles. The micelles provide higher glycerol miscibility on apolar solvents and interaction with the surface area of the TAG to form MAG and DAG (Arranz‐Martínez et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Kinetic study of liquid lipase‐catalyzed glycerolysis of olive oil using Lipozyme TL 100L

Finco

Fiametti

Lobo

et al. 2022

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Monoacylglycerol (MAG) and diacylglycerol (DAG) are two natural components found in most edible oils and fats. Conventional synthesis of MAG and DAG is usually conducted by glycerolysis of triacylglycerol (TAG) at high temperatures (above 200°C) in the presence of an alkaline catalyst. In this work, the synthesis of MAG and DAG using enzymatic glycerolysis of olive oil was investigated using Tween 80 as surfactant, n‐butanol as co‐surfactant and the novel lipase in free/liquid formulation Lipozyme TL 100L as catalyst. Experimental design was used to evaluate the effect of enzyme load and reaction temperature on the feedstock conversion. Enzyme load and system temperature were significant variables in the statistical design and the best condition was found at 35°C, 7.5 vol% of Lipozyme TL 100L and glycerol to oil volumetric ratio of 2:1 with conversion of TAG at approximately 98% after 2 h of process. A mathematical model based on the Ping‐Pong Bi‐Bi mechanism was used to describe the reaction kinetics. The model adequately described the behavior of the system and can be a useful tool for the design of reactors in larger scales.

show abstract

Lipase catalyzed glycerolysis of ratfish liver oil at stirred tank basket reactor: A kinetic approach

Cited by 15 publications

References 32 publications

Kinetic study of liquid lipase-catalyzed glycerolysis of olive oil using Lipozyme TL 100L

Kinetic study of liquid lipase-catalyzed glycerolysis of olive oil using Lipozyme TL 100L

Kinetic Modeling of a Consecutive Enzyme-Catalyzed Enantioselective Reaction in Supercritical Media

Kinetic study of liquid lipase‐catalyzed glycerolysis of olive oil using Lipozyme TL 100L

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