Lipase catalyzed modification of fish oil to incorporate capric acid

Jennings, Brenda H.; Akoh, Casimir C.

doi:10.1016/s0308-8146(00)00266-1

Cited by 37 publications

(26 citation statements)

References 11 publications

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“…The conditions applied by these authors for the enzymatic reaction are in agreement with other results found in the literature for similar substrates (Jennings and Akoh, 1999;Jennings and Akoh, 2001). In the latter work, these authors have observed that a high incorporation of capric acid into fish oil was achieved at an enzyme load of 5 % (by mass of substrates) for the solvent-free reaction using the lipase Lipozyme ® RM IM.…”

Section: Resultssupporting

confidence: 89%

See 1 more Smart Citation

Incorporation of medium chain fatty acids into fish oil triglycerides by chemical and enzymatic interesterification

Feltes¹,

Pitol²,

Correia³

et al. 2009

Grasas y Aceites

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Section: Resultssupporting

confidence: 89%

“…TAGs were produced with an average of 28.8 mol% of C10:0, predominantly located in the end positions (Jennings and Akoh, 2001).…”

Section: Incorporation Of Medium Chain Fatty Acids Into Fish Oil Trigmentioning

confidence: 99%

Incorporation of medium chain fatty acids into fish oil triglycerides by chemical and enzymatic interesterification

Feltes¹,

Pitol²,

Correia³

et al. 2009

Grasas y Aceites

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“…The fatty acid composition (Table 1) of fish oil was consistent with the date published data for fish oil fatty acids (Haraldsson and Hjaltason, 2001;Jennings and Akoh, 2001), with relatively high contents in the n-3 PUFA of nutritional interest, eicosapentaenoic (EPA) and docosahexaenoic acids (DHA). After hydrolysis for 24 h, followed by urea complexation, the contents of EPA and DHA increased, respectively, approximately by 40% and 50% compared to the original fish oil.…”

Section: Hydrolysis Of Fi Sh Oil and Urea Concentration Of N-3 Pufa Bsupporting

confidence: 86%

Nutritional enrichment of vegetable oils with long-chain n-3 fatty acids through enzymatic interesterification with a new vegetable lipase

2015

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SUMMARY:The aim of the present work was to produce vegetable oils enriched with long-chain n-3 fatty acids of nutraceutical interest, through an enzyme-catalyzed interesterification with a new lipase, from physic nut (Jatropha curcas L.). The Vegetable Lipase Powder (biocatalyst) called VLP, which has never been applied in functional foods, was obtained from the physic nut seed, and efficiently hydrolyzed the 95% of waste fish oil in 24 h. Urea precipitation was used to concentrate polyunsaturated fatty acids (PUFA) and was further interesterified with oils of different sources by means of enzymatic catalysis. After the interesterification reaction, which was also catalyzed by the VLP, the PUFA content in coconut oil increased almost ten-fold from 1.8% to 17.7%. In palm oil, the PUFA content increased two-fold from 10.5% to 21.8%, while in olive oil the level of PUFA increased from 8.6% to 21.3%. The mixture of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (3.7% to 3.9%) was incorporated into the triacylglycerol fraction of each of the coconut, palm and olive oils. Through the hydroesterification (hydrolysis followed by interesterification) all the interesterified vegetable oils tested presented sufficient EPA and DHA levels to satisfy the levels recommended for intake by human adults in one tablespoon. KEYWORDS:Interesterification; Jatropha curcas L.; Lipase; Polyunsaturated fatty acids; Structured lipids RESUMEN: Enriquecimiento nutricional de aceites vegetales con ácidos grasos n-3 de cadena larga mediante interesterificación enzimática con una nueva lipasa vegetal. El objetivo del presente trabajo fue producir aceites vegetales enriquecidos con ácidos grasos n-3 de cadena larga de interés nutraceutico, por interesterificación catalizada mediante una nueva lipasa, una enzima de semilla de Jatropha curcas L. La lipasa vegetal en polvo (biocatalizador) llamada VLP, nunca ha sido aplicada en alimentos funcionales, se obtuvo mediante procedimientos físicos con semillas de nueces, e hidrolizó eficientemente el 95% de aceites de residuos de pescado en 24 h. La precipitación con urea se utilizó para concentrar los ácidos grasos poliinsaturados (PUFA) que fueron posteriormente interesterificados con aceites de diferentes fuentes mediante catálisis enzimática. Tras la reacción de interesterificación, que también fue catalizada por la VLP, el contenido de PUFA en el aceite de coco aumentó casi diez veces de 1,8% a 17,7%. En el aceite de palma, el contenido de PUFA aumentó dos veces desde 10,5% a 21,8%, mientras que en el aceite de oliva el nivel de PUFA incrementó de 8,6% a 21,3%. La mezcla de ácido eicosapentaenoico (EPA) y ácido docosahexaenoico (DHA) (3,7% a 3,9%) fué incorporada a la fracción de triacilgliceroles de cada uno de los aceites, coco, palma y oliva. Mediante hidroesterificación (hidrólisis seguido de interesterificación) todos los aceites vegetales interesterificados ensayados contienen en una cucharada suficientes niveles de EPA y DHA para cubrir los niveles recomendados de ingesta de huma...

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“…[8][9][10] Biochemical production of triaclyglycerols composed of long-and middlechains was facilitated and their mass production has been realized. 11,12) However, study of enzymatic synthesis of triacylglycerols that contain mixtures of shortand long-chain fatty acids is hampered by the unfavorable substrate specificity of lipase toward short-chain fatty acids.…”

mentioning

confidence: 99%

Acidolysis between Triolein and Short-Chain Fatty Acid by Lipase in Organic Solvents

Tsuzuki

2005

Bioscience, Biotechnology, and Biochemistry

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Ten kinds of lipases were examined as biocatalysts for the incorporation of short-chain fatty acids (acetic, propionic, and butyric acids) into triolein in order to produce one kind of reduced-calorie structured lipids. Trans-esterification (acidolysis) was successfully done in n-hexane by several microbial lipases. Among them, lipase from Aspergillus oryzae was used to investigate the effects of incubation time, substrate molar ratio, and water content on acidolysis. Finally, more than 80% of triolein was incorporated by butyric acid (molar ratio of triolein to butyric acid, 1:10) in the dried n-hexane at 52 C for 72 h. More than 90% of the products was monosubstituent, which was esterified with this short chain fatty acid at the 1-position of the glycerol moiety of triolein. These results suggest that A. oryzae lipase would be a powerful biocatalyst for the synthesis of low caloric oil, such as triacylglycerol containing a mixture of long-and short-chain aliphatic acids.

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Lipase catalyzed modification of fish oil to incorporate capric acid

Cited by 37 publications

References 11 publications

Incorporation of medium chain fatty acids into fish oil triglycerides by chemical and enzymatic interesterification

Incorporation of medium chain fatty acids into fish oil triglycerides by chemical and enzymatic interesterification

Nutritional enrichment of vegetable oils with long-chain n-3 fatty acids through enzymatic interesterification with a new vegetable lipase

Acidolysis between Triolein and Short-Chain Fatty Acid by Lipase in Organic Solvents

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