Lipase-Catalyzed Modification of Rice Bran Oil To Incorporate Capric Acid

Abstract

Capric acid (C10:0) was incorporated into rice bran oil with an immobilized lipase from Rhizomucor miehei as the biocatalyst. Effects of incubation time, substrate mole ratio, enzyme load, and water addition on mole percent incorporation of C10:0 were studied. Transesterification was performed in an organic solvent, hexane, and under solvent-free condition. Pancreatic lipase-catalyzed sn-2 positional analysis and tocopherol analysis were performed before and after enzymatic modification. Products were analyzed by gas-liquid chromatography (GLC) for fatty acid composition. After 24 h of incubation in hexane, there was an average of 26.5 +/- 1.8 mol % incorporation of C10:0 into rice bran oil. The solvent-free reaction produced an average of 24.5 +/- 3.7 mol % capric acid. In general, as the enzyme load, substrate mole ratio, and incubation time increased, the mole percent of capric acid incorporation also increased. Time course reaction indicated C10:0 incorporation increased up to 27.0 mol % at 72 h, for the reaction in hexane, and up to 29.6 mol % at 12 h, for the solvent-free reaction. The highest C10:0 incorporations (53.1 and 43.2 mol %) for the mole ratio experiment occurred at a mole ratio of 1:8 for solvent and solvent-free reactions, respectively. The highest C10:0 incorporation (27.9 mol %) for the reaction in hexane occurred at 10% enzyme load, and the highest incorporation (34.4 mol %) for the solvent-free reaction occurred at 20% enzyme load. Incorporation of C10:0 into rice bran oil declined with the addition of increasing amounts of water after reaching 30.3 mol % at 2% water addition in hexane, and in the solvent-free reaction after reaching 35.9 mol %.

Structured lipids from rice bran oil and stearic acid using immobilized lipase from Rhizomucor miehei

“…b-Tocopherol and b-tocotrienol were not detected. Similar losses have been reported in another study while incorporating capric acid into RBO [31].…”

Lipase catalyzed ester synthesis for food processing industries

“…Pancreatic lipase catalyzed Sn-2 positional analysis and tocopherol analysis were performed before and after enzymatic modification. When the enzyme load, substrate mole ratio and incubation time increased, the mole percent of capric acid incorporation also increased (Jennings et al, 2000). (Goto et al, 1995).…”

“…In the transesterification with the immobilized lipase from R. miehei for incorporation of carpic acid in the rice bran oil, hexane and solvent free system were analyzed. The incorporation rate declined with the increasing amount of the water for both the conditions (Jennings and Akoh, 2000). The effect of fatty alcohol on the kinetics of lauric acid esterification with lipase catalyzation was studied by Shintre et al (2002) Lowric acid has been esterified with some C 1 -C 18 aliphatic alcohols by a commercial lipase, Lipolase 100L, using isooctane as a solvent.…”

Production of MLM‐Type Structured Lipids Catalyzed by Immobilized Heterologous Rhizopus oryzae Lipase

“…The identification and quantification of the esterified fatty acids at the sn-2 position in the structured triacylglycerols was assayed following the pancreatic lipase hydrolysis procedure, adapted from Jennings and Akoh [14]. Two milliliters of 1 M Tris buffer (adjusted to pH 8.0 with HCl), 0.5 mL of 0.05% (w/v) sodium cholate solution, 0.2 mL of 2.2% (w/v) calcium chloride solution and 20 mg of pancreatic lipase were added to TAG samples.…”

“…Also, Lipozyme TL IM, a commercial immobilized lipase from Thermomyces lanuginosa, was used for the acidolysis of corn oil with caprylic acid in n-hexane: under optimized conditions, 21.5 mol% of caprylic acid was incorporated [13]. Jennings and Akoh [14] modified rice bran oil with capric acid, using Lipozyme IM as the biocatalyst: after 24 h of the reaction, 26.5 and 24.5 mol% incorporation of capric acid were obtained in hexane and in solvent-free media, respectively.…”