Lipase-catalyzed synthesis of a sugar ester containing arachidonic acid

Lipase-catalyzed regioselective acylation of sucrose in two-solvent mixtures

“…Our previous experience in the acylation of sucrose with trichloroethyl esters demonstrated the extraordinary improvement of the transesterification rate by immobilization of the enzyme on Celite (Cruces et al, 1992;Plou et al, 1995). The same effect has been reported by other researchers (Ward et al, 1997). Adsorption of enzymes onto macroscopic surface generates a better distribution of the the biocatalyst, exposing the enzyme more efficiently to the substrate.…”

Quantitative enzymatic production of 1,6‐diacyl sorbitol esters

“…Methods based on formation of phenylboronic complexes with sugars (Schlotterbeck et al, 1993) containing single free primary hydroxyl group may be exploited for selective monoacylation reactions in apolar media such as n-heptane (Oguntimein et al, 1993;Scheckermann et al, 1995). Alternatively, production of sugar monoesters from the isopropylidene sugar and the corresponding fatty acid may be carried out in absence of solvent (Ward et al, 1997). This procedure requires previous synthesis of the isopropylidene sugar from sugar and acetone by mild acid catalysis, and ultimately liberation of the free sugar ester by mild acid hydrolysis.…”

Synthesis of Surfactants Using Enzymes

“…It is noteworthy that lipases are able to accept acetals and boronic acid derivatives, which are both rather bulky substrates. Among the lipases used are those from Rhizomucor miehei , Chromobacterium viscosum, Pseudomonas and Candida antarctica [25][26][27].…”