Lipase from Brassica napus L. discriminates against cis-4 and cis-6 unsaturated fatty acids and secondary and tertiary alcohols

Enrichment of ?-linolenic acid from fungal oil by lipase-catalysed reactions

“…Lipases from oilseed rape (Hills et al 1989(Hills et al , 1990a and those from M. miehei (Hills et al 1989(Hills et al , 1990bRangheard et al 1989), Candida rugosa, porcine pancreas, as well as Geotrichum candidum (Rangheard et al 1989) have been shown to discriminate against y-linolenic acid in lipase-catalysed estefification of fatty acids with primary alcohols, such as n-butanol or n-propanol. Similarly, lipase from R. delemar has been found to discriminate against y-linolenoyl moieties and 7-1inolenic acid in the lipase-catalysed transesterification of various triacylglycerols with stearic acid and of trioleoylglycerol with various fatty acids, respectively (Osterberg et al 1989).…”

“…Similarly, lipase from R. delemar has been found to discriminate against y-linolenoyl moieties and 7-1inolenic acid in the lipase-catalysed transesterification of various triacylglycerols with stearic acid and of trioleoylglycerol with various fatty acids, respectively (Osterberg et al 1989). Moreover, lipase from oilseed rape was shown to catalyse the hydrolysis of tri-y-linolenoylglycerol at a much lower rate than trioleoylglycerol (Hills et al 1990a). These substrate selectivities of the lipases from oilseed rape and M. miehei have been utilized for the enrichment of y-linolenic acid from evening primrose oil by selective esterification of the fatty acids of this oil with n-butanol or by selective hydrolysis of the oil to yield, respectively, fatty acids or acyl-…”

Plant lipases and their applications in oils and fats modification

“…A study showed that this lipase had a preference for the hydrolysis of ricinoleic acid which constitutes about 90% of castor bean oil but did not exhibit any regioselectivity [6]. Rapeseed lipase (Brassica napus) which is located in the lipid bodies has been also studied and according to Hills et al it was shown to discriminate against cis-4 and cis-6 unsaturated fatty acids [7]. Other lipases were also detected in various oil seeds such as peanut, linseed or soya.…”

“…Examples of enrichment of γ-linolenic acid via selective hydrolysis of evening primrose oil were given. It was observed that the use of rapeseed lipase allowed the increase from 10 to 28% of γ-linolenic fatty acid content in the non-hydrolysed acylglycerols [7,19]. Similarly it was possible to exploit this lipase typoselectivity to develop a selective esterification process in which an alcoholysis reaction of cod liver oil with butanol was carried out in order to enrich the acylglycerols fraction in DHA [20].…”

Plant lipases: Biocatalyst aqueous environment in relation to optimal catalytic activity in lipase‐catalyzed synthesis reactions

“…To date, mostly microbial and animal lipases were used in preference to plant lipases (Macrae and Hammond, 1985;Muderhwa et al, 1989;Seitz, 1974). Nowadays, the tendency is leading towards the use of plant lipases (Caro et al, 2000 a,b;Giordani et al, 1991;Hills et al, 1990;Jachmania n et al, 1996;Mangos et al, 1999;Mukherjee, 1990Mukherjee, , 1994Mukherjee and Kiewitt, 1996Rao et al, 1992;Steinke et al, 2000;Villeneuve et al, 1995Villeneuve et al, , 1997aVilleneuve and Foglia, 1997). More and more plant extracts present interesting enzyme activities and, in particular lipase activity.…”