Lipase from marine Aspergillus awamori BTMFW032: Production, partial purification and application in oil effluent treatment

Abstract

Marine fungus BTMFW032, isolated from seawater and identified as Aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. In this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. Medium with soyabean meal-0.77% (w/v); (NH(4))(2)SO(4)-0.1m; KH(2)PO(4)-0.05 m; rice bran oil-2% (v/v); CaCl(2)-0.05 m; PEG 6000-0.05% (w/v); NaCl-1% (w/v); inoculum-1% (v/v); pH 3.0; incubation temperature 35°C and incubation period-five days were identified as optimal conditions for maximal lipase production. The time course experiment under optimized condition, after statistical modeling, indicated that enzyme production commenced after 36 hours of incubation and reached a maximum after 96 hours (495.0 U/ml), whereas maximal specific activity of enzyme was recorded at 108 hours (1164.63 U/mg protein). After optimization an overall 4.6-fold increase in lipase production was achieved. Partial purification by (NH(4))(2)SO(4) precipitation and ion exchange chromatography resulted in 33.7% final yield. The lipase was noted to have a molecular mass of 90 kDa and optimal activity at pH 7 and 40°C. Results indicated the scope for potential application of this marine fungal lipase in bioremediation.

An insight into microbial lipases and their environmental facet

“…PB, in combination with RSM, has resulted in optimizing the medium and increasing the enzyme yield from a multitude of yeasts, fungi, and bacteria. Rhizopus delemar (Acıkel et al 2010), A. carneus (Kaushik et al 2006), Aspergillus awamori (Basheer et al 2011), Geotrichum sp. (Burkert et al 2004), C. cylindracea (Salihu et al 2011), Burkholderia sp.…”

Detergent compatible alkaline lipase produced by marine Bacillus smithii BTMS 11

“…The crude enzyme extract obtained after SmF was purified by (NH 4 ) 2 SO 4 precipitation (20-90 %), followed by dialysis; and ion-exchange chromatography (Basheer et al 2011). The precipitated protein was resuspended in 50 mM Tris-HCl buffer (pH 8), dialysed against 0.01 M solution of the same buffer for 24 h, at 4°C, and assayed for lipase activity and protein content.…”

“…Enzyme characterization by SDS-PAGE and Zymography was performed by the procedures outlined by Basheer et al (2011). Samples precipitated with (NH 4 ) 2 SO 4 and active fractions collected after ion exchange chromatography, were electrophoresed by Native-PAGE and SDS-PAGE in a 10 % polyacrylamide gel according to the method of Laemmli (1970).…”

A novel report of fungal pathogen Aspergillus awamori causing black gill infection on Litopenaeus vannamei (pacific white shrimp)

“…The SEM analysis evidenced the A. awamori KM434331 colonies with conidiophores, conidial heads, globose to sub-globose, conidia covering the whole surface of the conidiophore which were similar to that of pathogenic fungi A. awamori BTMFW032 (Beena et al, 2010 and the identity of phylogenetic results showed 100% sequence similarity with the previously available sequences of A. awamori (Basheer et al, 2011).…”