Lipase immobilization on activated and functionalized carbon for the aroma ester synthesis

Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols

“…Polymeric carrier of Novozym 435 features higher affinity to organic solvents, whereas silica to water. However, despite the good affinity of Novozyme 435 for organic solvents, its stability is rather poor [24][25][26][27].…”

“…However, the major disadvantage of polymer carriers is their low stability in organic solvents, which was shown among others by Zhao, Jose and Kowalczykiewicz [24][25][26]. For this very reason the search for stable, hydrophobic carriers for lipase is still an important issue [27]. Mesoporous silicates (MS) prepared by templating method, and mesoporous cellular foams (MCF) in particular, demonstrate considerable potential for enzyme immobilization [28][29][30].…”

Immobilization of Phenylalanine Ammonia-lyase via EDTA Based Metal Chelate Complexes – Optimization and Prospects

“…Enzyme immobilization can enhance resistance against the conditions of use (pH, temperature, organic solvents) and give an opportunity for reuse and application in continuous flow mode [4][5][6][7][8][9]. Nowadays there are several well-known methods for enzyme immobilization: covalent immobilization [6,7,[10][11][12][13][14], adsorption [15,16], embedding in polymer [17][18][19] or silica-based matrices [20,21]. New methods have emerged as well over the last decades, in which specifically tagged enzymes can be immobilized via specific interactions [22][23][24][25].…”

Effect of Different TiO2 Morphologies on the Activity of Immobilized Lipase for Biodiesel Production

“…Enzymatic catalysts are employed in different industrial applications, including ester synthesis reactions. 1 Enzymatic catalysts are preferred over chemical catalysts because chemical catalysts have many drawbacks, such as high energy consumption, low product purity, and generation of wastewater. 2 Enzymes play an effective role in industrial biotechnology and microbiology.…”

“…The most common and preferred technique in lipase immobilization is physical adsorption on various supports via interfacial activation (hydrophobic interaction) because of the low cost and the ease and simplicity of the technique without a need to activate the support. 1 , 3 The support should be chemically, mechanically, and thermally stable; insoluble in the solution involved in the technique; cheap; and compatible with the enzyme to be immobilized. 8 …”