Lipase immobilization on amino-silane modified superparamagnetic Fe3O4 nanoparticles as biocatalyst for biodiesel production

Miao, Changlin; Yang, Lingmei; Wang, Zhongming; Luo, Wen; Li, Huiwen; Lv, Pengmei; Yuan, Zhenhong

doi:10.1016/j.fuel.2018.02.149

Cited by 114 publications

(34 citation statements)

References 30 publications

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“…However, the covalent bonds between the enzyme and the magnetic support could lead to the stabilization of the configuration for the bound lipase and the improvement of its stability. Considering the reusability study, the long-term stability for the immobilized lipase is really comparable to that reported in the previous work [19,23,[33][34][35].…”

Section: Reusability Of the Immobilized Lipasesupporting

confidence: 85%

“…Additionally, the naked magnetic nanoparticles always show instability over longer periods of time because of their sensitivities to oxidation [17,18]. To address these issues, appropriate passive porous materials, such as carbon materials, silica, and organic polymer, are reported to be coated on the magnetic nanoparticles in an attempt to greatly suppress their aggregation and also to effectively enhance their chemical stabilities [19,20]. The thus-formed core-shell structured composites, consisting of a magnetite core and a porous material shell, are of particular interest due to the comprehensive advantages of their individual components.Among the porous materials, metal-organic frameworks (MOFs), produced through self-assembly of metal clusters with interconnected organic linkers, have become regarded as excellent catalyst supports owing to their highly ordered crystalline structure, tunable porosity, and large surface area [21].…”

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confidence: 99%

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Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Xie

Huang

2019

Catalysts

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In this research, core–shell structured Fe3O4@MIL-100(Fe) composites were prepared by coating Fe3O4 magnetite with porous MIL-100(Fe) metal-organic framework (MOF) material, which were then utilized as magnetic supports for the covalent immobilization of the lipase from Candida rugosa through amide linkages. By using the carbodiimide/hydroxysulfosuccinimide (EDC/NHS) activation strategy, the lipase immobilization efficiency could reach 83.1%, with an activity recovery of 63.5%. The magnetic Fe3O4@MIL-100(Fe) composite and immobilized lipase were characterized by several techniques. The characterization results showed that the Fe3O4 core was coated with MIL-100(Fe) shell with the formation of perfect core–shell structured composites, and moreover, the lipase was covalently tethered on the magnetic carrier. The immobilized lipase displayed a strong magnetic response and could be facilely separated by an external magnetic field. With this magnetic biocatalyst, the maximum biodiesel conversion attained 92.3% at a methanol/oil molar ratio of 4:1, with a three-step methanol addition manner, and a reaction temperature of 40 °C. Moreover, the biocatalyst prepared in the present study was recycled easily by magnetic separation without significant mass loss, and displayed 83.6% of its initial activity as it was reused for five runs, thus allowing its potential application for the cleaner production of biodiesel.

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Section: Reusability Of the Immobilized Lipasesupporting

confidence: 85%

mentioning

confidence: 99%

Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Xie

Huang

2019

Catalysts

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“…For MNP-NH 2 (Fig. 1a), the peaks observed at 3170 cm −1 and 3113 cm −1 , and 1686 cm −1 are mainly attributed to stretching and bending vibrations of the primary amine (-NH 2 ), respectively [22]. In Fig.…”

Section: Characterization Of Mnp-calb Exmentioning

confidence: 92%

“…Similarly, Macario et al [21] synthesized lipaseencapsulated silica nanoparticles for the transesterification of triolein to produce biodiesel. In another study, Miao et al [22] prepared a biocatalyst by immobilization of lipase on amino-functionalized magnetic nanoparticles (MNPs). The prepared catalyst was tested for FAME synthesis from rapeseed oil.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel synthesis from palm fatty acid distillate using enzyme immobilized on magnetic nanoparticles

Gupta

Rathod

2020

SN Appl. Sci.

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The present study deals with the esterification of palm fatty acid distillate (PFAD) using immobilized lipase on magnetic nanoparticles (MNPs) to produce biodiesel in a cleaner and more environmentally friendly way. Commercially available lipase CALB EX was used for immobilization of lipase on MNPs. The effect of various reaction parameters including the methanol-to-PFAD molar ratio, biocatalyst loading, reaction temperature, and agitation speed was examined using a one-factor-at-a-time approach. Maximum PFAD conversion of 82.74% was achieved under mild reaction conditions, with a methanol-to-PFAD molar ratio of 1.6:1, biocatalyst loading of 8 wt% (lipase content 0.45 wt%), agitation speed of 150 rpm, reaction temperature of 50 °C, and reaction time of 10 h. The catalytic activity of MNP-CALB EX was compared with commercial Amberlyst-15 under similar reaction conditions. It was observed that MNP-CALB EX achieved 2.6-fold higher conversion than Amberlyst-15. The reusability of the immobilized biocatalyst was also tested to determine cost feasibility. It was observed that the immobilized biocatalyst could be used up to five cycles, with residual conversion of 80.19% in the fifth cycle.

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“…Knowing it as a renewable, biodegradable, noninflammable, and nontoxic product, the biodiesel has been studied as an attractive substitute to the diesel fuel based on petroleum. Lipase immobilized on magnetic particles has been used to produce biodiesel by enzymatic transesterification [44,45]. Moreover, magnetic microreactors employing immobilized enzymes are widely investigated to study and manipulate bioprocesses.…”

Section: Biotechnological Applicationsmentioning

confidence: 99%

Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Cabrera¹,

Neri²,

Soria³

et al. 2019

Applied Surface Science

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In this chapter, the authors will show different proposals of magnetic bio-derivatives and its applicability in biotechnology. The historical context of immobilized enzymes, as well as highlighting the main advantages and disadvantages of each, will be mentioned. Besides, iron oxides and composite materials will be presented as support for biomolecules immobilization. Composites are effortlessly prepared including many materials capable of providing advantages to the magnetic derivatives. Enzymes covalently linked to these magnetic particles combine their catalytic properties with reaction specificity, reusability, and possible reactor construction. In addition, proteins can also be purified by these magnetic composites containing specific ligands allowing reactors and reuses too. Some characterization techniques used to study the magnetic material and derivative immobilized will be described as well. Altogether, an engaging presentation about the interesting features of magnetic bio-derivatives will highlight their uses in biotechnology field as well as in others.

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Lipase immobilization on amino-silane modified superparamagnetic Fe3O4 nanoparticles as biocatalyst for biodiesel production

Cited by 114 publications

References 30 publications

Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Biodiesel synthesis from palm fatty acid distillate using enzyme immobilized on magnetic nanoparticles

Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

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