Lipase Immobilized Metal‐Organic Frameworks as Remarkably Biocatalyst for Ester Hydrolysis: A One Step Approach for Lipase Immobilization

Abstract

Recently, development of smart materials for immobilization of enzyme draws a significant attention in frontier research due to their applications in a wide range of catalytic reactions in several industries. Although numerous supports have already been developed in the past years for enzyme immobilization, challenges still exist to design a material with superior enticing properties like green synthesis approach, high enzyme loading and efficient immobilization process. Here, we have reported an efficient method to immobilize Candida rugosa lipase (CRL) in Zn-(NH 2 -BDC) metal-organic frameworks (MOFs) via physical adsorption. The main objective of this work is to develop a material for enzyme immobilization that can immobilize enzyme during the synthesis of support. Here, a single step procedure offers both the synthesis of support and enzyme immobilization process. After successful immobilization, it is observed from the systematic characterization that the CRL is loaded 280 mg/g of Zn-(NH 2 -BDC) MOFs during the crystal growth. The immobilized CRL demonstrates promising stability and catalytic activity in the ester hydrolysis. Most importantly, the immobilized CRL retains 79% of its initial activities after 10 times reuse. Our study highlights to develop an elegant material for effective enzyme immobilization.

Sustainability of biodiesel production using immobilized enzymes: A strategy to meet future bio‐economy challenges

“…Different catalysts have been recognized and tested with lipase to produce biodiesel. The most prominent are nanofibers, 153 polymeric monoliths, 154 mesoporous materials, 155 metal‐organic frameworks (MOFs), 156 nanomaterials like carbon nanotubes, 157 halloysite nanotubes, 158 SnO 2 hollow nanotubes, 159 magnetic nanoparticles like iron oxide nanoparticles, 160 polymer‐coated magnetic nanoparticles, 161 silica‐coated magnetic nanoparticles, 162 then in recent times, ionic liquids (IL's) functionalized magnetic nanoparticles 163 and MOFs modified magnetic nanoparticles. Therefore, the environmental impact, economics, and scale‐up implementation of immobilized biodiesel production require a better understanding of production patterns through technological tools and economic aspects 149 .…”

Recent Advances in the Strategies of Simultaneous Enzyme Immobilization Accompanied by Nanocarrier Synthesis

“…However, most of these immobilization strategies typically involve the preparation of carrier materials for enzyme loading, followed by the physical or chemical binding of the carrier and enzyme. The preparation and separation of carriers involve multiple steps and cumbersome processes, which are time-consuming [26]. Therefore, there is a growing research interest in developing strategies that can simultaneously synthesize carrier materials and immobilize enzymes.…”