Lipase immobilized on ionic liquid-functionalized magnetic silica composites as a magnetic biocatalyst for production of  trans -free plastic fats

Xie, Wenlei; Zang, Xuezhen

doi:10.1016/j.foodchem.2018.03.010

Cited by 132 publications

(50 citation statements)

References 39 publications

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“…In the IR spectrum of the immobilized lipase, there were two IR absorption bands at 1642 cm −1 and 1551 cm −1 to be observed as shown in Figure 2c. Even though these two IR bands were probably overlapped with the COO − anti-symmetric and symmetric stretching vibration absorption bands, they mainly originated from amide I and II of the lipase [15,18]. Furthermore, the characteristic IR bands assignable to the Fe 3 O 4 @MIL-100(Fe) support were also found for the immobilized lipase.…”

Section: Enzymatic Activities Of the Immobilized Lipasementioning

confidence: 92%

“…From a sustainability point of view, it is still of great demand to fabricate a novel enzyme support with the aim of improving the separation performance of the immobilized lipase. Quite recently, magnetic materials, when used as enzyme supports, have attracted great research attention since the magnetite-loaded enzyme can allow solid-liquid separation to be done fast and facilely, with minimal mass loss, by applying an external magnet [15,16]. Unfortunately, magnetic nanoparticles may easily aggregate into large clusters, owing to their magnetic dipole-dipole attractions.…”

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confidence: 99%

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Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Xie

Huang

2019

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In this research, core–shell structured Fe3O4@MIL-100(Fe) composites were prepared by coating Fe3O4 magnetite with porous MIL-100(Fe) metal-organic framework (MOF) material, which were then utilized as magnetic supports for the covalent immobilization of the lipase from Candida rugosa through amide linkages. By using the carbodiimide/hydroxysulfosuccinimide (EDC/NHS) activation strategy, the lipase immobilization efficiency could reach 83.1%, with an activity recovery of 63.5%. The magnetic Fe3O4@MIL-100(Fe) composite and immobilized lipase were characterized by several techniques. The characterization results showed that the Fe3O4 core was coated with MIL-100(Fe) shell with the formation of perfect core–shell structured composites, and moreover, the lipase was covalently tethered on the magnetic carrier. The immobilized lipase displayed a strong magnetic response and could be facilely separated by an external magnetic field. With this magnetic biocatalyst, the maximum biodiesel conversion attained 92.3% at a methanol/oil molar ratio of 4:1, with a three-step methanol addition manner, and a reaction temperature of 40 °C. Moreover, the biocatalyst prepared in the present study was recycled easily by magnetic separation without significant mass loss, and displayed 83.6% of its initial activity as it was reused for five runs, thus allowing its potential application for the cleaner production of biodiesel.

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Section: Enzymatic Activities Of the Immobilized Lipasementioning

confidence: 92%

mentioning

confidence: 99%

Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Xie

Huang

2019

Catalysts

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“…However, the number of these is limited and so there are efforts to discover novel enzymes and optimisation of their inherent properties such as stability, reaction rate and specificity, to enable the development of new green industrial processing methods. Uses of enzymes in food processing strictly follows green chemistry approaches, including avoiding use of costly harsh chemicals in various food processing activities [3,4]. Exploring novel robust enzymes sourced from recombinant/wild microbial strains can provide new industrially useful catalysts for food or industrial processes.…”

Section: Introductionmentioning

confidence: 99%

Suitability of Recombinant Lipase Immobilised on Functionalised Magnetic Nanoparticles for Fish Oil Hydrolysis

et al. 2019

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Recombinant Bacillus subtilis lipase was immobilised on magnetic nanoparticles by a facile covalent method and applied to fish oil hydrolysis. High loading of enzyme to the functionalised nanoparticle was achieved with a protein binding efficiency of 95%. Structural changes of the confined enzyme on the surface of the nanoparticles was investigated using transmission electron microscopy and spectroscopic techniques (attenuated total reflectance-Fourier transform infrared and circular dichroism). The biocatalytic potential of immobilised lipase was compared with that of free enzyme and biochemically characterised with respect to different parameters such as pH, temperature, substrate concentrations and substrate specificity. The thermal stability of functionalised nanoparticle bound enzyme was doubled that of free enzyme. Immobilised lipase retained more than 50% of its initial biocatalytic activity after recyclability for twenty cycles. The ability to the immobilised thermostable lipase to concentrate omega-3 fatty acids from fish oil was investigated. Using synthetic substrate, the immobilised enzyme showed 1.5 times higher selectivity for docosahexaenoic acid (DHA), and retained the same degree of selectivity for eicosapentaenoic acid (EPA), when compared to the free enzyme.

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“…It has been reported that potential detrimental health problems, such as increased risk factors of coronary heart disease, are related to the intake of semisolid plastic fats containing trans ‐fatty acids (FA) generally from partially hydrogenated oils (Dhaka et al, ). Structured lipids (SL) are a class of triacylglycerols (TAG) that have been restructured to incorporate new FA into original vegetable oils or alter their composition and positional distribution of FA in the glycerol backbone (Xie and Zang, ). Medium‐chain triacylglycerols (MCT), in which constituent FA are medium‐chain fatty acids (MCFA) and long‐chain FA, are beneficial fats and have been investigated for medical, nutritional, and food applications (Abed et al, ).…”

Section: Introductionmentioning

confidence: 99%

Polymeric Acidic Ionic Liquid‐Functionalized SBA‐15 as a Solid Catalyst for Production of Low‐Calorie Structured Lipids

Xie

Zhang

Wang

2018

J Americ Oil Chem Soc

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The aim of this study is to develop a clean and environmentally friendly process for production of lowcalorie structured lipids in accordance with the requirement of cleaner production and sustainable developments. To achieve this, polymeric acidic ionic liquid (IL)functionalized SBA-15 catalysts were prepared by surface grafting copolymerization of IL 1-propylsulfonate-3-vinylimidazolium hydrogen sulfate onto the SBA-15 support using azobisisobutyronitrile (AIBN) as an initiator. The as-prepared solid catalysts were fully characterized using several techniques. Results demonstrated that the polymeric acidic IL was successfully tethered on the SBA-15 silica, and the mesoporous structure of the SBA-15 support remained intact after the grafting copolymerization. The solid catalyst showed high activities in the soybean oil acidolysis with capric and caprylic acids. The acidolysis products were analyzed by a gas chromatographic method, and the incorporation of medium-chain fatty acids into soybean triacylglycerols was 53.4% over this solid catalyst. Furthermore, the solid catalyst could be recycled several times without appreciable loss of its activity.Keywords Structured lipid Á Heterogeneous catalyst Á Ionic liquid Á Acidolysis Á SBA-15 Á Medium-chain fatty acids J Am Oil Chem Soc (2018) 95: 1549-1559.

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Lipase immobilized on ionic liquid-functionalized magnetic silica composites as a magnetic biocatalyst for production of trans -free plastic fats

Cited by 132 publications

References 39 publications

Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Enzymatic Production of Biodiesel Using Immobilized Lipase on Core-Shell Structured Fe3O4@MIL-100(Fe) Composites

Suitability of Recombinant Lipase Immobilised on Functionalised Magnetic Nanoparticles for Fish Oil Hydrolysis

Polymeric Acidic Ionic Liquid‐Functionalized SBA‐15 as a Solid Catalyst for Production of Low‐Calorie Structured Lipids

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