Lipase in biodiesel production

Chioke, Onyinyechukwu J.; Ogbonna, Christiana N.; Onwusi, C.C.; Ogbonna, James C.

doi:10.5897/ajbr2018.0999

Cited by 10 publications

(2 citation statements)

References 98 publications

(98 reference statements)

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“…FAAE yields of 62.17, 59.68, 73.11, 83.64 and 83.68 % when methanol, ethanol, propanol, 1-butanol, and 2-butanol were applied in the reaction, respectively (Figure 2). The FAAE increased with increasing length of the carbon chain of the alcohols [32]. The maximum FAAE yield of 86.80 % was obtained, when iso-butanol was used in the reaction.…”

Section: Effects Of Acyl Acceptor On Synthesis Of Fatty Acid Alkyl Es...mentioning

confidence: 92%

Production of Methanol Tolerant Cell-bound Lipase of Magnusiomyces spicifer SPB2 and Application as Whole-cell Biocatalyst in Transesterification Reaction

Srimhan

Hongpattarakere²

2023

Trends Sci

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Cell-bound lipase (CBL) is a potential biocatalyst for production of fatty acid alkyl ester (FAAE), which is feasible replacement for diesel fuel. This study aimed to improve both hydrolysis and transesterification activities of CBL from the yeast Magnusiomyces spicifer SPB2, which could be employed as whole-cell biocatalyst for transesterification reactions through optimizing the growth medium using Taguchi orthogonal array experimental method (L9). The optimized medium, resulting the highest CBL transesterification activity, consisted of soybean oil, peptone, Gum Arabic and an initial pH of 5. Interestingly, the fatty acid methyl ester (FAME) yield increased up to 33.28 times when compared with non-optimized medium. Moreover, cell morphology of M. spicifer SPB2 varied depending on the culture medium component. The single cell was more active to be used as the whole-cell biocatalyst than the pseudomycelial form for transesterification reaction. The highest FAME yield was 93.86 % when refined palm oil to methanol molar ratio of 1:8 was used as substrates. Whole-cell lipase from M. spicifer showed favorable activity to iso-butanol as an acyl accepter, resulting in fatty acid butyl ester (FABE) yield of 86.80 % at 1:3 molar ratio of palm oil to iso-butanol. The FAME and FABE yields dramatically decreased from 93.86 and 86.80 % to 57.02 and 4.48 %, respectively after the reuse of the enzyme to the second batch of reaction. These results indicated that the whole-cell biocatalyst developed from M. spicifer was an efficient and economical approach to improve enzyme activity and stability in the transesterification reaction. HIGHLIGHTS Methanol tolerant cell-bound lipase and extracellular lipase of spicifer SPB2 were produced The whole-cell biocatalyst of spicifer SPB2 was developed for FAME production The optimized medium was developed to prepare whole-cell lipases from spicifer SPB2 Gum Arabic was the most influencing factor for synthetic activity of whole-cell CBL from yeast Single cell morphology exhibited higher transesterification activity than pseudomycelial form GRAPHICAL ABSTRACT

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Section: Effects Of Acyl Acceptor On Synthesis Of Fatty Acid Alkyl Es...mentioning

confidence: 92%

Production of Methanol Tolerant Cell-bound Lipase of Magnusiomyces spicifer SPB2 and Application as Whole-cell Biocatalyst in Transesterification Reaction

Srimhan

Hongpattarakere²

2023

Trends Sci

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“…Lipase enzymes are used in the process of transesterification and esterification due to economic and environmental reasons. This is because lipases have the ability to use monoglycerides, diglycerides and triglycerides as well as free fatty acids [12].…”

Section: Introductionmentioning

confidence: 99%

Lipase Production of Aspergillus aculeatus MS. 11 Using Solid State Fermentation on Rubber Seed Press Cake

Nurjanah¹,

Ilmi²

2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Lipase is an enzyme that has various use in industrial sector. However, the enzyme production cost is considerably high mainly due to expensive fermentation substrate. Therefore, it is necessary to develop effective media formulation to increase production of lipase enzyme without reducing the amount of production. This research aims to know the effectiveness of rubber seed press cake as a fermentation substrate for Aspergillus aculeatus Ms. 11. We observed the effect of glucose and olive oil as a carbon source addition on the substrate, determined the growth profile and lipase production on the substrate in lipase production. Isolates of Ms.11 was inoculated in 14,28 mL MGM medium with substrate variation rubber seed press cake, rubber seed press cake + glucose, rubber seed press cake + olive oil, and combination substrate (rubber seed press cake + glucose + olive oil) during 0, 2, 4, 7 days. The highest lipase activity and lipase productivity in this study shown at 19.23 U/mL and 186.51 Yield/day, there were obtained from rubber seed press cake + olive oil on day 2, whereas the highest specific activity of lipase enzyme (1.16 U/mg) was obtained in substrate variation rubber seed press cake + olive oil on day 7. We concluded that maximum lipase production of Ms. 11 can be obtained using rubber seed press cake with addition of olive oil.

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Production of Next-Generation Biodiesel from High Yielding Strains of Microorganisms: Recent Advances

Adetunji

Olaniyan

Okeke

2020

Nanotechnology in the Life Sciences

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Lipase in biodiesel production

Cited by 10 publications

References 98 publications

Production of Methanol Tolerant Cell-bound Lipase of Magnusiomyces spicifer SPB2 and Application as Whole-cell Biocatalyst in Transesterification Reaction

Production of Methanol Tolerant Cell-bound Lipase of Magnusiomyces spicifer SPB2 and Application as Whole-cell Biocatalyst in Transesterification Reaction

Lipase Production of Aspergillus aculeatus MS. 11 Using Solid State Fermentation on Rubber Seed Press Cake

Production of Next-Generation Biodiesel from High Yielding Strains of Microorganisms: Recent Advances

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