Lipase production by diverse phylogenetic clades of Aureobasidium pullulans

Leathers, Timothy D.; Rich, Joseph O.; Anderson, Amber M.; Manitchotpisit, Pennapa

doi:10.1007/s10529-013-1268-5

Cited by 28 publications

(16 citation statements)

References 29 publications

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“…as reported by Bigey et al [37]; from Mucor mucedo by Stern et al [38] and Mucor sp. by Abbas et al [39]; from Scopulariopsis brevicaulis by Nagy et al [40] which was able to resolve racemic 1-phenylethanol rac-1a and racemic 1-cyclohexylethanol rac-1b displaying its biocatalytic activity and enantiomer selectivity; and from Aureobasidium pullulans by Leathers et al [41]. As shown in Table 1, Strain C3 (Rhizopus oryzae) had the highest lipase activity.…”

Section: Resultsmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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“…Some other fungi, for instance, C. rugosa (Lakschmi et al, 1999;Serra et al, 1992) and several aspergilli (Reese et al, 1955) are known to be able to grow on linseed and olive oils. It is likely that A. melanogenum is able to use oil as nutrition, since it is known to produce lipase (Chi et al, 2009;Leathers et al, 2013;Leelaruji et al, 2013;Liu et al, 2008;Wang et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Also, other microorganisms (precursors) can colonize the wood, enabling growth of A. melanogenum. It seems likely that A. melanogenum can use oil as a nutritient since it is known to produce lipase, an enzyme that hydrolyzes oil molecules (triglycerides) into the potential carbon sources glycerol and fatty acids (Chi et al, 2009;Leathers et al, 2013;Leelaruji et al, 2013;Liu et al, 2008;Wang et al, 2007;Wongwatanapaiboon et al, 2016). Moreover, Aureobasidium is known to grow on paints containing linseed oil (Bardage, 1998;Harvath et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

Oil type and cross‐linking influence growth of Aureobasidium melanogenum on vegetable oils as a single carbon source

et al. 2018

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Aureobasidium melanogenum is the main fungus found in a spontaneously formed biofilm on a oil-treated wood. This dark colored biofilm functions as a protective coating. To better understand biofilm formation, in this study A. melanogenum was cultured on olive oil and raw linseed oil. Metabolic activity and oil conversion were measured. The results show that A. melanogenum is able to grow on linseed oil and olive oil as a single carbon source. The fungus produces the enzyme lipase to convert the oil into fatty acids and glycerol. Metabolic activity and oil conversion were equal on linseed oil and olive oil. The fungus was not able to grow on severe cross-linked linseed oil, meaning that the degree of cross-linking of the oil is important for growth of A. melanogenum. Dark coloring of the colony was seen on linseed oil, which might be a stress response on the presence of autoxidation products in linseed oil. The colony on olive oil showed delayed melanin production indicating an inhibitory effect of olive oil on melanin production.

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“…As reported above, enzymes may play a role as antimicrobials, but enzyme production and enzymatic activity have important roles in various biotechnological applications. A. pullulans is reported to produce amylases [35], cellulases [36], lipases [37], xylanases [38,39], proteases [40][41][42], laccase [43] and mannanases [44]. Currently in the wine industry, pectinases, glucanases, xylanases and proteases are used to improve the clarification and processing of wine.…”

Section: Enzymesmentioning

confidence: 99%

The Multiple and Versatile Roles of Aureobasidium pullulans in the Vitivinicultural Sector

Bozoudi

Tsaltas

2018

Fermentation

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The saprophytic yeast-like fungus Aureobasidium pullulans has been well documented for over 60 years in the microbiological literature. It is ubiquitous in distribution, being found in a variety of environments (plant surfaces, soil, water, rock surfaces and manmade surfaces), and with a worldwide distribution from cold to warm climates and wet/humid regions to arid ones. Isolates and strains of A. pullulans produce a wide range of natural products well documented in the international literature and which have been regarded as safe for biotechnological and environmental applications. Showing antagonistic activity against plant pathogens (especially post-harvest pathogens) is one of the major applications currently in agriculture of the fungus, with nutrient and space competition, production of volatile organic compounds, and production of hydrolytic enzymes and antimicrobial compounds (antibacterial and antifungal). The fungus also shows a positive role on mycotoxin biocontrol through various modes, with the most striking being that of binding and/or absorption. A. pullulans strains have been reported to produce very useful industrial enzymes, such as β-glucosidase, amylases, cellulases, lipases, proteases, xylanases and mannanases. Pullulan (poly-α-1,6-maltotriose biopolymer) is an A. pullulans trademark product with significant properties and biotechnological applications in the food, cosmetic and pharmaceutical industries. Poly (β-l-malic acid), or PMA, which is a natural biopolyester, and liamocins, a group of produced heavy oils and siderophores, are among other valuable compounds detected that are of possible biotechnological use. The fungus also shows a potential single-cell protein source capacity with high levels of nucleic acid components and essential amino acids, but this remains to be further explored. Last but not least, the fungus has shown very good biocontrol against aerial plant pathogens. All these properties are of major interest in the vitivinicultural sector and are thoroughly reviewed under this prism, concluding on the importance that A. pullulans may have if used at both vineyard and winery levels. This extensive array of properties provides excellent tools for the viticulturist/farmer as well as for the oenologist to combat problems in the field and create a high-quality wine.

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Lipase production by diverse phylogenetic clades of Aureobasidium pullulans

Cited by 28 publications

References 29 publications

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Oil type and cross‐linking influence growth of Aureobasidium melanogenum on vegetable oils as a single carbon source

The Multiple and Versatile Roles of Aureobasidium pullulans in the Vitivinicultural Sector

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