Lipase Production by <i>Aspergillus niger</i> 11T53A14 in Wheat Bran Using Experimental Design Methodology

Abstract

Lipases have various industrial applications and their production by filamentous fungi in solid state fermentation (SSF) process is of interest. In this work the lipase enzyme production by the mutant Aspergillus niger 11T51A14, which is a well-known lipase producer was studied using wheat bran as substrate. Microorganism used in fermentation process was inoculated at a concentration of 10 7 spores/g m into the medium containing nitrogen (ammonium sulfate), sunflower soapstock and wheat bran. The SSF process for enzyme production was carried out in aerated columns immersed in a water bath at 32ºC for 72 h. The process was studied using a central composite rotatable design 2 2 , where variables tested were the nitrogen concentration (0.32-0.88 % w/w) and the volume of liquid (60.9-89.1 mL). The lipase activity was analyzed in enzymatic extracts. The wheat bran, in the fermentation medium, acted as a carbohydrate source and as an inducer for the lipase production. The maximum enzyme activity found was 153.4 U/g dm , with a nitrogen concentration of 0.6 % and 89.1 mL of volume of liquid. The response surface model demonstrated that higher volume of liquid had a positive effect in lipase activity, while nitrogen concentration showed an optimal range (0.4-0.6%).

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

“…The strains studied achieved maximum lipase activity with 25% sugarcane bagasse combined with 75% wheat bran or soybean bran at 40% moisture content. The optimum fermentation time for lipase production was 96 h. The effectiveness of wheat bran in the fermentation medium, as a carbon source and inducer for the production of lipase, was evaluated by Santos et al [114]. Aspergillus niger 11T51A14 was inoculated onto the medium containing nitrogen (ammonium sulphate), sunflower soapstock and wheat bran.…”

<b>Filamentous fungi producing enzymes under fermentation in cassava liquid waste

“…This low availability stimulates the mechanism of expression of the enzymes needed to generate simple sugars. In addition, excessive fermentation time can lead to nutritional depletion of the medium, implying the reduction of microbial growth, making the conversion of biomass into products unviable and, consequently, reducing the production of enzymes (Santos, Muruci, Damaso, Silva, & Santos, 2014).…”

Solid state fermentation process with Aspergillus kawachii enhances the cancer-suppressive potential of silkworm larva in hepatocellular carcinoma cells

“…Filamentous fungi have been traditionally used as a food resource in the forms of miso, cheese, and alcoholic beverages due to the high efficiency of various kinds of enzyme production [30]. Especially, well-known fungi such as Penicillium restrictum , Canadida rugose , Colletotrichum gloeosporioides , Aspergillus niger , and Sporobolomyces ruberrimus have been reported to produce fungal lipases, which catalyze hydrolysis of triglycerides to fatty acids and glycerol [31]. In addition, lipase production of filamentous fungi in solid state fermentation is affected by numerous factors such as culture media, nitrogen sources, oils, carbohydrates, pH, O 2 , CO 2 , temperature, and metal ions [31, 32].…”

“…Especially, well-known fungi such as Penicillium restrictum , Canadida rugose , Colletotrichum gloeosporioides , Aspergillus niger , and Sporobolomyces ruberrimus have been reported to produce fungal lipases, which catalyze hydrolysis of triglycerides to fatty acids and glycerol [31]. In addition, lipase production of filamentous fungi in solid state fermentation is affected by numerous factors such as culture media, nitrogen sources, oils, carbohydrates, pH, O 2 , CO 2 , temperature, and metal ions [31, 32]. These results indicate that Aspergillus kawachii hydrolyzed the rich lipid proportion of silkworm larvae to increase fatty acid contents during the fermentation process.…”