Lipases: An overview of its current challenges and prospectives in the revolution of biocatalysis

Synthesis of mitomycin analogs catalyzed by coupling of laccase with lipase and the kinetic model

“…The experimental results demonstrated that both the internal and external mass transfer resistance could be negligible with 0.45 mm diameter of two immobilized enzymes and 150 rpm speed, thus the kinetic model established was an inherent reaction kinetic model. Concentration of n-butyl amine, mmol L -1 [C 0 ] Initial concentration of n-butyl amine, mmol L -1 K 1 , K 8 , K 12 Kinetic parameter, dimensionless K 2 , K 3 , K 5 , K 6 , K 7 , K 9 , K 10 Kinetic parameter, L mmol −1 K 4 , K 11 Kinetic parameter, L 2 mmol −2 K 13 , K 16 Kinetic parameter, h −1 U −1 mL K 14 , K 15 Kinetic parameter, h −1 mmol −1 U −1 mL ∑K 1 , ∑K 2 Sum of Kappa constant P 1 Product 1, 2-methyl-1,4-benzoquinone P 2 Product 2, 2-methyl-3-n-butylaminoyl-4-hydro-1-quinone P 3 Product 3, 2-methyl-3-n-butylaminoyl-1,4-benzoquinone www.soci.org Y Zhang et al…”

“…Although reaction (1) seems to work only in the presence of one enzyme, i.e. laccase, P 1 , the product of reaction (1), is the substrate of reaction (2), and reaction (2) is catalyzed by the second enzymelipase. When P 1 is consumed by reaction (2), product inhibition of reaction (1) is reduced or even eliminated.…”

“…Lipases are versatile tools for biocatalysis which have broad specificity and relatively better stability than other enzymes in organic solvents. 1 The catalytic potential of lipases is being increasingly observed for C─C bond formation as well as C-heteroatom bond formation such as C─N, C─O, C─S coupling and so on. 2 The Michael reaction is one of the most important strategies for the formation of a C─C bond, and it has been widely used to generate valuable building blocks in organic synthesis.…”

“…14 Amination mediated by laccase proves to be an efficient way which can generate biological active ingredients such as aminonaphthoquinone or aminoquinone derivatives. 15,16 The mitomycin-like compounds such as 5-alkylamino- [1,4]-benzoquinones and 2,5-bis(alkylamino)- [1,4]-benzoquinones, were synthesized by heteromolecular coupling of 2-methoxy-3-methylhydroquinone with primary amines under the catalysis of Myceliophthora thermophile laccase (MtL) and Pycnoporus cinnabarinu laccase (PcL), respectively. 17 The productivity of this process catalyzed by MtL was only 32.9% for monoaminated products as the main product.…”

“…Without this kinetic model analysis, only increasing the amount of lipase would not increase the yield of P 3 because reaction (3) catalyzed by lipase was not a limiting step, and this is the advantage of a kinetic model of the cascade reaction. In this reaction system, the product of reaction (1) is P 1 which can inhibit reaction (1). Lipase-catalyzed reaction (3) consumes P 1 rapidly, thus eliminating product inhibition and making reaction (1) proceed rapidly.…”