Lipases: Useful biocatalysts for the preparation of pharmaceuticals

Gotor‐Fernández, Vicente; Brieva, Rosario; Gotor, Vicente

doi:10.1016/j.molcatb.2006.02.010

Cited by 329 publications

(153 citation statements)

References 47 publications

Supporting

Mentioning

140

Contrasting

Unclassified

Order By: Relevance

“…Cold-adapted lipases are used in the manufacture of single-isomer chiral drugs (Gotor-Fernandez et al 2006a). Cold active lipase from C. antarctica or Pseudomonas sp.…”

Section: Medical and Pharmaceutical Applicationsmentioning

confidence: 99%

Cold active lipases – an update

Kavitha

2016

Frontiers in Life Science

View full text Add to dashboard Cite

Cold active lipases (CLPs) are gaining importance nowadays as they are increasingly used in fine chemical synthesis, bioremediation, food processing and as detergent additive. These enzymes exhibit high catalytic activity at low temperatures and flexibility to act at low water medium. Since they are active at low temperatures consume less energy and also stabilize fragile compounds in the reaction medium. CLPs are commonly obtained from psychrophilic microorganisms which thrive in cold habitats. Compared to mesophilic and thermophilic lipases, only a few CLPs were studied and industrially exploited so far. CLPs (C. antarctica lipase-A and C. antarctica lipase-B) from Candida antarctica isolated from Antarctic region are the well studied and industrially employed, and many are being followed up. This review updates the CLPs reported recently and the industrial applications of CLPs. ARTICLE HISTORY

show abstract

“…Cold-adapted lipases are used in the manufacture of single-isomer chiral drugs (Gotor-Fernandez et al 2006a). Cold active lipase from C. antarctica or Pseudomonas sp.…”

Section: Medical and Pharmaceutical Applicationsmentioning

confidence: 99%

Cold active lipases – an update

Kavitha

2016

Frontiers in Life Science

View full text Add to dashboard Cite

show abstract

“…The versatility of these biocatalysts, when used in organic solvents, allows attainment of a great variety of other pharmaceutical intermediates. 3,4 Each application requires specific properties of the lipases with respect to substrate specificity, stability, temperature and pH-dependence. Filamentous fungi are the most preferred sources of lipases as they might produce extracellular enzymes, facilitating extraction from fermentation growth media.…”

Section: Introductionmentioning

confidence: 99%

Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study

Silva

Contesini

Carvalho

2008

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Lipase de Aspergillus niger foi imobilizada por deposição utilizando Celite e as atividades de hidrólise e esterificação, a estabilidade e a enantioseletividade das formas livre e imobilizada foram comparadas. Ambas as preparações mostraram propriedades bioquímicas similares, com atividade máxima em pH 6,0 e temperatura de 30-40 o C. Não foram observadas diferenças na atividade hidrolítica em função do pH para as duas formas da enzima. Os efeitos mais importantes observados após a imobilização foram a estabilidade térmica e a melhoria da atividade de esterificação na reação do (R,S)-ibuprofeno com 1-propanol em presença do isooctano. Além disso, a lipase na forma imobilizada manteve, pelo menos, 73% de sua atividade de esterificação após 5 dias de armazenamento a 40 o C e pôde ser reciclada e reutilizada por pelo menos 6 vezes.Aspergillus niger lipase was immobilized by deposition on Celite and the hydrolytic and esterification activities, stability and enantioselectivity of both free and immobilized enzyme were compared. Both the free and immobilized preparations showed similar biochemical properties, with maximum activity at pH 6.0 and a temperature of 30-40 o C. The most important effects observed when the lipase was immobilized were thermal stability and an improved esterification activity during the reaction of (R,S)-ibuprofen with 1-propanol in isooctane. Moreover, immobilized Aspergillus niger lipase maintained an esterification activity of at least 73% after 5 days of storage at 40 o C and can be recycled and reused at least 6 times.

show abstract

“…Also, Candida antartica lipase B is a very effective catalyst for the production of amines and amides using different enzymatic procedures. The commercial availability and recycling possibility make CALB lipase as an ideal tool for the synthesis and resolution of wide range of nitrogenated compounds that can be used in industrial sector 50,51 . The primary factor limiting the industrial use of CALB has most likely been the relatively expensive enzymes for immobilization.…”

Section: Introductionmentioning

confidence: 99%