Lipid Alterations after Cell Wall Inhibition. Fatty Acid Content of Streptococcus pyogenes and Derived L-Form<sup>*</sup>

Panos, Charles; Cohen, Murray; Fagan, G.

doi:10.1021/bi00869a003

Cited by 33 publications

(19 citation statements)

References 16 publications

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“…The predominant saturated fatty acid in the L-form remained palmitic acid during the adaptation from osmotic fragility to osmotic stability. Qualitatively, the fatty acid composition of the established L-form growing at various levels of sodium chloride was similar to that reported previously for this b-form from hypertonic medium (14), except that linoleic acid was absent. Our improved extraction procedure has eliminated most of this dienoic acid which is absorbed from the medium.…”

supporting

confidence: 85%

Adaptation of an osmotically fragile L-form of Streptococcus pyogenes to physiological osmotic conditions and its ability to destroy human heart cells in tissue culture

Leon¹,

Panos²

1976

Infect Immun

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An osmotically fragile L-form of Streptococcus pyogenes, type 12, was quickly rendered osmotically stable by decreasing the sodium chloride content of the growth medium and with the temporary use of oleic acid. The change from osmotic-fragility to stability was accompanied by changes in cell yield, generation time, saturated/unsaturated fatty acid ratio of the membrane, and cytoplasmic protein composition. Finally, this resulting osmotically stable L-form survived and was capable of rapidly destroying Girardi human heart cells in tissue culture.

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supporting

confidence: 85%

Adaptation of an osmotically fragile L-form of Streptococcus pyogenes to physiological osmotic conditions and its ability to destroy human heart cells in tissue culture

Leon¹,

Panos²

1976

Infect Immun

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“…These changes are similar to those reported by Panos et al. (7) in S. pyogenes and its derived L-form.…”

Section: Methodssupporting

confidence: 91%

Fatty Acid Composition of L-Forms of Streptococcus faecalis Cultured at Different Osmolalities

1973

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The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T.8) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T581) cultured in BHI without sucrose; and (iii) an unstable L-form (T,) cultured in BHI with 0.5 M sucrose and 1,000 U of penicillin per ml.L-forms were obtained by centrifugation and lysed by washing in 1 mM tris(hydroxymethyl)aminomethane-hydrochloride buffer. The parent S. faecalis was also cultured in BHI and BHI containing 0.5 M sucrose, and washed with buffer. The fatty acid composition of L-forms of S. faecalis cultured in BHI without sucrose (370 mosmol) had higher Ci: l and lower C16 than L-forms cultured in the same media with added 0.5 M sucrose (950 mosmol) in both exponential and stationary cultures. In the stationary phase of growth, C1,, was

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“…It would seem that either the membrane plays a greater role in bacteria in accommodating the variation in osmotic pressures or the L-form membrane has become more resilient by some means. Difference in fatty-acid composition of L-forms as compared to the parent bacteria suggest that considerable modification of lipids occurs (5). We have observed changes in membrane fatty acids at different stages of growth of T53 (unpublished data).…”

Section: Discussionmentioning

confidence: 82%

Osmotic Stability and Sodium and Potassium Content of L-Forms of Streptococcus faecalis

et al. 1972

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A stable L-form of Streptococcus faecalis (T53) was transferred in media containing decreasing concentrations of sucrose until it grew in medium without added osmotic stabilizer. This L-form (designated T53,) was compared with T53 from which it was derived. The survival of these two L-forms suspended at different osmolalities showed that maximal survival for T58I was 350 to 400 milliosmolal and for T,3 was 900 to 1,000 milliosmolal. Both peaks were at the osmolality of their growth media. Measurement of intracellular potassium and sodium showed that the concentration of these ions was reduced in T,3,1.

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Lipid Alterations after Cell Wall Inhibition. Fatty Acid Content of Streptococcus pyogenes and Derived L-Form^*

Cited by 33 publications

References 16 publications

Adaptation of an osmotically fragile L-form of Streptococcus pyogenes to physiological osmotic conditions and its ability to destroy human heart cells in tissue culture

Adaptation of an osmotically fragile L-form of Streptococcus pyogenes to physiological osmotic conditions and its ability to destroy human heart cells in tissue culture

Fatty Acid Composition of L-Forms of Streptococcus faecalis Cultured at Different Osmolalities

Osmotic Stability and Sodium and Potassium Content of L-Forms of Streptococcus faecalis

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Lipid Alterations after Cell Wall Inhibition. Fatty Acid Content of Streptococcus pyogenes and Derived L-Form*

Cited by 33 publications

References 16 publications

Adaptation of an osmotically fragile L-form of Streptococcus pyogenes to physiological osmotic conditions and its ability to destroy human heart cells in tissue culture

Adaptation of an osmotically fragile L-form of Streptococcus pyogenes to physiological osmotic conditions and its ability to destroy human heart cells in tissue culture

Fatty Acid Composition of L-Forms of Streptococcus faecalis Cultured at Different Osmolalities

Osmotic Stability and Sodium and Potassium Content of L-Forms of Streptococcus faecalis

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Lipid Alterations after Cell Wall Inhibition. Fatty Acid Content of Streptococcus pyogenes and Derived L-Form^*