Lipid Analyses: Part I Properties of fats, oils and lipids: recovery and basic compositional studies with gas–liquid chromatography and thin-layer chromatography

Ackman, R. G.; Ratnayake, W. M. N.

doi:10.1016/b978-0-12-718051-9.50015-9

Cited by 11 publications

(3 citation statements)

References 247 publications

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“…These compounds were readily identified from the GC and MS data. In a preliminary GC analysis (methyl esters) on Carbowax 20 M, the equivalent chain length (ECL) values determined (17.23 for the hexadecatrienoic acid and 18.97 for the octadecatrienoic acid) were in good concordance with those given in the literature ( , ). The first fatty acid pyrrolidide was eluted from the DB-1 column after the pentadecanoyl pyrrolidide.…”

Section: Resultssupporting

confidence: 83%

Fatty Acid Distribution in Muscle, Liver, and Gonads of Rays (Dasyatis marmorata, Rhinobatos cemiculus, and Rhinoptera marginata) from the East Tropical Atlantic Ocean

Kebir

Barnathan

Siau

et al. 2003

J. Agric. Food Chem.

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If a great number of rays are fished in the Tropical East Atlantic Ocean for their caudal fins, only a small amount of ray flesh is processed. Among them, three species of rays, Dasyatis marmorata, Rhinobatos cemiculus, and Rhinoptera marginata, from the Mauritanian coast have been investigated for the fatty acid composition of their lipids. Gas chromatography and gas chromatography-mass spectrometry allowed identification of 50 molecules from muscles, livers, and gonads of these fishes. Principal component analysis, starting from >50 samples, reveals significant differences in various fatty acid distributions, related to the species and sex of the sampled fish. Some of them are preferentially present in one sex or in both species, whereas the occurrence of others characterizes the male and female of one or two species. The results show that rays are potential resources of polyunsaturated fatty acids (PUFA) and should be used in the diet of local populations. The lipidic fractions contained a high amount of PUFA (up to 30% of the total), mainly composed of docosa-4,7,10,13,16,19-hexaenoic acid, eicosa-5,8,11,14-tetraenoic acid, and eicosa-5,8,11,14,17-pentaenoic acid.

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Section: Resultssupporting

confidence: 83%

Fatty Acid Distribution in Muscle, Liver, and Gonads of Rays (Dasyatis marmorata, Rhinobatos cemiculus, and Rhinoptera marginata) from the East Tropical Atlantic Ocean

Kebir

Barnathan

Siau

et al. 2003

J. Agric. Food Chem.

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“…The analysis of FA in biological samples involves mainly three steps: extraction of lipids into an organic solvent (11,12), acid-or base-catalyzed derivatization to obtain volatile FAME (13,14), and quantification of individual FAME by GC with FID detection (15,16). However, there is no validated method for the analysis of the FA content in placental tissue (9,10,17).…”

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confidence: 99%

Analysis of FA contents in individual lipid fractions from human placental tissue

Klingler

Demmelmair

Larqué

et al. 2003

Lipids

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Data on FA contents in the human placenta are limited. Different methods have been used for the FA analysis, and only percentage results have been presented. We developed and evaluated a method for the determination of FA concentrations in placental tissue. Lipids were extracted from placental tissue with a chloroform/methanol mixture; and phospholipids (PL), nonesterified FA (NEFA), TG, and cholesterol esters (CE) were isolated by TLC. Individual lipid fractions were derivatized with methanolic hydrochloric acid, and the FAME were quantified by GC with FID. The CV of intra-assay (n = 8) of absolute concentrations were evaluated for FA showing a tissue content > 0.01 mg/g. CV ranges were 4.6-11.0% for PL, 6.4-9.3% for NEFA, 6.1-8.9% for TG, and 11.4-16.3% for CE. The relative FA composition across a term placenta indicated no differences between samples of central and peripheral locations of maternal and fetal site (CV 0.5-9.9%), whereas the absolute FA concentrations were only reproducible in the PL fraction (CV 7.0-12.8%). The method shows a reasonably high precision that is well suited for physiological and nutritional studies.

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“…This method gives an error of approximately 1% in the final energy value of the sample (data not shown). Analysis of lipid class composition was performed by Iatroscan TLC/FID (Ackman and Ratnayake, 1989). Different lipid classes were identified against standard runs containing corn oil (triacylglycerols; Mazola, Best Foods Canada, Inc., Etobicoke, ON, Canada), cholesterol oleate (Sigma Chemical Co., St. Louis, MO), stearic acid (Applied Science Laboratories Inc., State College, PA), phosphatidyl choline (Sigma Chemical Co.), and cholesterol (Sigma Chemical Co.).…”

Section: Methodsmentioning

confidence: 99%

Measuring Total Lipid Content in Rat Carcasses: A Comparison of Commonly Employed Extraction Methods

Brooks¹,

Ratnayake²,

Lampi³

et al. 1998

J. Agric. Food Chem.

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Intermethod differences between four common procedures for measuring carcass lipid content, lipid composition, and lipid energy value were determined: chloroform−methanol extraction, saponification plus hexane extraction, dichloromethane−methanol extraction (Soxhlet) and hexane extraction (Goldfisch). Samples were either freeze-dried or air-dried prior to Soxhlet or Goldfisch extraction. Carcass lipid content ranged from 7.8 (chloroform−methanol) to 8.8 (saponification) g/100 g carcass: the lowest and highest values were significantly different from each other but not from other procedures. The energy value of the extracted lipid ranged from 8804 (dichloromethane−methanol) to 9176 (saponification) kcal/g. Lipid class compositional analysis and fatty acid profile analysis showed significant differences between all methods showing that these are best determined after extraction with chloroform−methanol. The results also illustrated that small discrepancies may exist between methods for measuring total carcasses lipid content and carcass lipid energy but showed that no single, best method exists for measuring these parameters. Keywords: Lipid extraction; methods; rat carcass

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Lipid Analyses: Part I Properties of fats, oils and lipids: recovery and basic compositional studies with gas–liquid chromatography and thin-layer chromatography

Cited by 11 publications

References 247 publications

Fatty Acid Distribution in Muscle, Liver, and Gonads of Rays (Dasyatis marmorata, Rhinobatos cemiculus, and Rhinoptera marginata) from the East Tropical Atlantic Ocean

Fatty Acid Distribution in Muscle, Liver, and Gonads of Rays (Dasyatis marmorata, Rhinobatos cemiculus, and Rhinoptera marginata) from the East Tropical Atlantic Ocean

Analysis of FA contents in individual lipid fractions from human placental tissue

Measuring Total Lipid Content in Rat Carcasses: A Comparison of Commonly Employed Extraction Methods

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