“…At present, how to maximize lipid yield, reduce the cost and increase manipulation flexibility dealing with the various technology bottlenecks of the lipid production process in microalgae cultivation are the important tasks (Delrue et al, 2013;Bellou et al, 2014;He et al, 2015a). Nutrient (especially nitrogen) and light regimes (i.e., incolumn density, photoperiod and light intensity) are the major environmental determinants of microalgae growth, metabolism and morphology, including the accumulation of lipids in the form of triacylglycerols (TAG) (Eberhard et al, 2008;Damiani et al, 2010;Wahidin et al, 2013;He et al, 2015a,b). As vigorous growth and lipid accumulation are usually mutually exclusive in naturally occurring microalgae, so optimizing the cultivation strategy is the cornerstone to achieve biodiesel production.…”