Lipid Bicelle Micropatterning Using Chemical Lift-Off Lithography

Belling, Jason N.; Cheung, Kevin M; Jackman, Joshua A.; Sut, Tun Naw; Allen, Matthew J.; Park, Jae Hyeon; Jonas, Steven J.; Cho, Nam‐Joon; Weiss, Paul S.

doi:10.1021/acsami.9b20617

Cited by 14 publications

(10 citation statements)

References 65 publications

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“…In the interfacial science field, bicelles have also proven to be useful as a lipid nanostructure to fabricate supported lipid bilayers (SLBs), which are extensively used in applications such as biosensors and micropatterned arrays 17 – 25 . Indeed, bicelle adsorption onto hydrophilic surfaces such as silicon dioxide can initiate a surface-mediated molecular assembly process that yields high-quality SLBs composed of long-chain phospholipid 26 , 27 .…”

Section: Introductionmentioning

confidence: 99%

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Sut

Yoon

Park

et al. 2020

Sci Rep

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Originally developed for the structural biology field, lipid bicelle nanostructures composed of longand short-chain phospholipid molecules have emerged as a useful interfacial science tool to fabricate two-dimensional supported lipid bilayers (SLBs) on hydrophilic surfaces due to ease of sample preparation, scalability, and versatility. To improve SLB fabrication prospects, there has been recent interest in replacing the synthetic, short-chain phospholipid component of bicellar mixtures with naturally abundant fatty acids and monoglycerides, i.e., lauric acid and monocaprin. Such options have proven successful under specific conditions, however, there is room for devising more versatile fabrication options, especially in terms of overcoming lipid concentration-dependent SLB formation limitations. Herein, we investigated SLB fabrication by using bicellar mixtures consisting of long-chain phospholipid and capric acid, the latter of which has similar headgroup and chain length properties to lauric acid and monocaprin, respectively. Quartz crystal microbalance-dissipation, epifluorescence microscopy, and fluorescence recovery after photobleaching experiments were conducted to characterize lipid concentration-dependent bicelle adsorption onto silicon dioxide surfaces. We identified that uniform-phase SLB formation occurred independently of total lipid concentration when the ratio of long-chain phospholipid to capric acid molecules ("q-ratio") was 0.25 or 2.5, which is superior to past results with lauric acid-and monocaprin-containing bicelles in which cases lipid concentration-dependent behavior was observed. Together, these findings demonstrate that capric acid-containing bicelles are versatile tools for SLB fabrication and highlight how the molecular structure of bicelle components can be rationally finetuned to modulate self-assembly processes at solid-liquid interfaces. Bicelles are an important class of membrane-mimicking lipid nanostructures that self-assemble from mixtures of long-and short-chain phospholipids under appropriate processing conditions 1,2. Also known as lipid nanodisks 3 or bilayered mixed micelles 4,5 , bicelles can exist in a wide range of morphologies (e.g., perforated sheets and wormlike micelles) depending on parameters such as temperature, q-ratio (long-to short-chain phospholipid molar ratio), total lipid concentration, and lipid composition, and are widely conceptualized as two-dimensional disks whereby long-chain phospholipids constitute a planar lipid bilayer surface and the short-chain phospholipids form a rimmed edge around the bilayer 6-12. Since they can exhibit magnetic alignment in some cases, bicellar disks have long been used in the nuclear magnetic resonance spectroscopy field as membrane protein hosts 4,13-16. In the interfacial science field, bicelles have also proven to be useful as a lipid nanostructure to fabricate supported lipid bilayers (SLBs), which are extensively used in applications such as biosensors and micropatterned arrays 17-25. Indeed, bicelle adsorption onto...

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Section: Introductionmentioning

confidence: 99%

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Sut

Yoon

Park

et al. 2020

Sci Rep

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“…43 However, these materials entail challenges associated with synthesis, material heterogeneity, device reproducibility, and robust functionalization. 38,80,81 Compared with other FET-based detection platforms, our functionalized transistors can be straightforwardly and reproducibly fabricated at the wafer scale in arrays using softlithographic techniques, 32,50,[62][63][64]82,83 increasing the potential for rapid translation and deployment toward screening for pandemic infection, clinical disease diagnostics, and personalized healthcare applications. 84 In contrast to FETs based on 2D materials, solution-processed ultrathin indium oxide films are highly uniform, 32,51,85 can be straightforwardly and selectively functionalized, 33,86 and have been demonstrated to have higher device sensitivities and lower detection limits in potentiometric biosensing applications (e.g., pH and glucose sensing) compared with other 2D (e.g., graphene, reduced graphene oxide, MoS 2 ) and 1D (e.g., carbon nanotube, Si nanowire) materials.…”

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confidence: 99%

Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors

et al. 2020

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We detect short oligonucleotides and distinguish between sequences that differ by a single base, using label-free, electronic field-effect transistors (FETs). Our sensing platform utilizes ultrathin-film indium oxide FETs chemically functionalized with single-stranded DNA (ssDNA). The ssDNA-functionalized semiconducting channels in FETs detect fully complementary DNA sequences and differentiate these sequences from those having different types and locations of single base-pair mismatches. Changes in charge associated with surface-bound ssDNA vs double-stranded DNA (dsDNA) alter FET channel conductance to enable detection due to differences in DNA duplex stability. We illustrate the capability of ssDNA-FETs to detect complementary RNA sequences and to distinguish from RNA sequences with single nucleotide variations. The development and implementation of electronic biosensors that rapidly and sensitively detect and differentiate oligonucleotides present new opportunities in the fields of disease diagnostics and precision medicine.

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“…Among the different options, the bicelle method has attracted widespread interest because it requires minimal freeze‐thaw‐vortex processing to form bicellar nanostructures composed of long‐ and short‐chain phospholipids, requires much lower bulk lipid concentrations to form SLBs compared to other methods, and works in fully aqueous conditions and across a wide range of environmental conditions 51–53 . While long‐chain phospholipids are abundantly available, one initially limiting aspect of the bicelle method was the need to include short‐chain phospholipids, which are far less common and relatively expensive; a commonly used one is 1,2‐dihexanoyl‐ sn ‐glycero‐3‐phosphocholine (DHPC) 29,53–55 . However, recent progress has demonstrated the potential to use bicellar nanostructures composed of long‐chain phopsholipids and low cost, widely available free fatty acids or monoglycerides to effectively and affordably fabricate SLBs 56–58 .…”

Section: Lipid Bilayer Fabrication Technologiesmentioning

confidence: 99%

“…[51][52][53] While long-chain phospholipids are abundantly available, one initially limiting aspect of the bicelle method was the need to include short-chain phospholipids, which are far less common and relatively expensive; a commonly used one is 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC). 29,[53][54][55] However, recent progress has demonstrated the potential to use bicellar nanostructures composed of long-chain phopsholipids and low cost, widely available free fatty acids or monoglycerides to effectively and affordably fabricate SLBs. [56][57][58] Hence, bicelles are…”

Section: Vesicle Fusionmentioning

confidence: 99%

Lipid coating technology: A potential solution to address the problem of sticky containers and vanishing drugs

Junren

Yoon

Sut

et al. 2021

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Pharmaceutical drugs and vaccines require the use of material containers for protection, storage, and transportation. Glass and plastic materials are widely used for packaging, and a longstanding challenge in the field is the nonspecific adsorption of pharmaceutical drugs to container walls -the so-called "sticky containers, vanishing drugs" problem -that effectively reduces the active drug concentration and can cause drug denaturation. This challenge has been frequently discussed in the case of the anticancer drug, paclitaxel, and the ongoing coronavirus disease 2019 (COVID-19) pandemic has brought renewed attention to this material science challenge in light of the need to scale up COVID-19 vaccine production and to secure sufficient quantities of packaging containers. To reduce nonspecific adsorption on inner container walls, various strategies based on siliconization and thin polymer films have been explored, while it would be advantageous to develop mass-manufacturable, natural material solutions, especially ones involving pharmaceutical grade excipients. Inspired by how lipid nanoparticles have revolutionized the vaccine field, in this perspective, we discuss the prospects for developing lipid bilayer coatings to prevent nonspecific adsorption of pharmaceutical drugs and vaccines and how recent advances in lipid bilayer coating fabrication technologies are poised to accelerate progress in the field. We critically discuss recent examples of how lipid bilayer coatings can prevent nonspecific sticking of proteins and vaccines to relevant material surfaces and examine future translational prospects.

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Lipid Bicelle Micropatterning Using Chemical Lift-Off Lithography

Cited by 14 publications

References 65 publications

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid

Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors

Lipid coating technology: A potential solution to address the problem of sticky containers and vanishing drugs

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